Source: NORTH DAKOTA STATE UNIV submitted to
VETERINARY DIAGNOSTIC LABORATORY
Sponsoring Institution
State Agricultural Experiment Station
Project Status
NEW
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
1022731
Grant No.
(N/A)
Project No.
ND04407
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 2020
Project End Date
Sep 30, 2025
Grant Year
(N/A)
Project Director
Pecoraro, HE, .
Recipient Organization
NORTH DAKOTA STATE UNIV
1310 BOLLEY DR
FARGO,ND 58105-5750
Performing Department
Veterinary Diagnostic Laboratory
Non Technical Summary
The North Dakota Veterinary Diagnostic Laboratory (VDL) is comprised of veterinarians and technicians that process and test samples to determine the cause of animal health problems in North Dakota. Data and interpretations generated from testing activities at the VDL are compiled in reports that are sent to clients, which include veterinarians, animal producers and the general public. The VDL works to provide timely and accurate diagnosis of animal disease to curtail the impact of losses due to animal mortality and morbidity. The lab participates at the local, national and international levels to achieve this goal. We are closely involved with animal and public health agencies in the state of North Dakota and engage in teaching and research at North Dakota State University.
Animal Health Component
100%
Research Effort Categories
Basic
25%
Applied
75%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113399116040%
3113499116010%
3113599116010%
3113699116010%
3113810116010%
3113840116010%
3113899116010%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3499 - Dairy cattle, general/other; 3840 - Laboratory animals; 3899 - Other animals, general; 3699 - Sheep and wool, general/other; 3599 - Swine, general/other; 3399 - Beef cattle, general/other; 3810 - Horses, ponies, and mules;

Field Of Science
1160 - Pathology;
Goals / Objectives
Major goals of this project include accurate and timely diagnosis of animal disease in North Dakota and the surrounding region. Submitted cases are from food animal, companion animal and exotic or zoo species. In addition, the VDL is a member laboratory of the National Animal Health Laboratory Network (USDA) and the Veterinary Laboratory Investigation and Response Network (FDA) that provide surveillance and testing capabilities for foreign animal diseases and issues affecting the animal food supply for the United States. The VDL is closely involved with state animal (North Dakota Board of Animal Health, North Dakota Fish and Game) and public health (North Dakota Department of Health) agencies to test for disease agents affecting both human and animal health. Finally the VDL also participates in research projects aimed at addressing issues of importance to North Dakota livestock producers.
Project Methods
The activities of the VDL are completed in the section laboratories (Toxicology, serology, histology, pathology, clinical pathology, bacteriology, mycology, molecular diagnostics and virology) that analyze submitted samples, produce tests results and provide interpretation of results back to clients in the form of a formal laboratory report.

Progress 10/01/22 to 09/30/23

Outputs
Target Audience:The target audience is practicing veterinarians, livestock producers, animal owners and the general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The NDSU VDL continues to train a veterinary anatomic pathology resident, who is now in year three of residency and preparing for specialty board examinations. In addition, we hosted a veterinary student intern who is currently enrolled as a first year student in a veterinary medicine program. We will have another pre-vet intern this spring and offer our services to teachveterinary diagnostic methods to graduate and undergraduate students. How have the results been disseminated to communities of interest?Results go out to clientele in the form of reports generated from samples submitted to the laboratory. On occasion, NDSU VDL professionals will give presentations to veterinary clientele on disease trends seen at the laboratory. A quarterly newsletter is published by the laboratory and distributed to practitioners across North Dakota and the region. What do you plan to do during the next reporting period to accomplish the goals?The VDL will continue its service to the state and region by testing samples, participating in NAHLN and Vet-LIRN, working closely with other state health agencies, and providing continuing education and outreach.

Impacts
What was accomplished under these goals? The laboratory processed over 11,000 accessions and performed ~100,000 tests last year including thousands of tests for agents of public health significance (e.g., zoonotic agents, anthrax, rabies). The NDSU VDL can be re-activated to test for SARS-CoV-2 (COVID-19) and other human diseases as needed by North Dakota Department of Health and Human Services. In addition, as part of the NAHLN response to foreign animal disease emergence, we test for highly pathogenic avian influenza in North Dakota poultry and wild birds. Laboratory outreach included participating in the North Dakota Veterinary Medical Association annual meetings, preparing and distributing quarterly lab newsletters, writing articles for the North Dakota Stockman's Association and other publications, providing learning opportunities for undergraduate and graduate students, and conducting laboratory tours to agricultural and educational groups. New toxicological, microbiological, and molecular assays under development or validation include: Pasteurella multocida typing on MALDI-TOF Biotyper In collaboration with other veterinary diagnostic laboratories, validation of new Brucella canis serological assays Bacterial whole genome sequencing of Salmonella in captive large felines and Pasteurella multocida in clinically ill calves Brain sodium method by ICP-OES Mineral and heavy metal analyses of liver biopsies by ICP-MS Bromethelin or related metabolite desbromethalin in animal tissues, stomach contents, and bait samples

Publications

  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Garc�a-Bernalt Diego J, Singh G, Jangra S, Handrejk K, Laporte M, Chang LA, El Zahed SS, Pache L, Chang MW, Warang P, Aslam S, Mena I, Webb BT, Benner C, Garc�a-Sastre A, Schotsaert M. Breakthrough infections by SARS-CoV-2 variants boost cross-reactive hybrid immune responses in mRNA-vaccinated Golden Syrian Hamsters. bioRxiv [Preprint]. 2023 May 23.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Fe�ler A, Want Y, Burbick C, Diaz-Campos D, Fajt VR, Lawhon SD, Li XL, Lubbers BV, Maddock K, Miller RA, Papich MG, Simjee S, Sweeney MT, Watts JL, Wu C, Shen J, Schwarz S. Antimicrobial susceptibility testing in veterinary medicine: Performance, interpretation of results, best practices, and pitfalls. One Health Advances 2023;1(26).
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Maddock KJ, Gefroh SJ, Burbick CR. ?-Lactam resistance in veterinary ?-hemolytic Streptococcus species: Are we experiencing a public health or test method crisis? JAVMA 2023;261(9):1403-6.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Ensley S, Mostrom M. Equine Mycotoxins. Vet Clin North Am Equine Pract. 2023 Dec 7:S0749-0739(23)00068-8. doi: 10.1016/j.cveq.2023.10.002. Epub ahead of print. PMID: 38061965.
  • Type: Book Chapters Status: Submitted Year Published: 2023 Citation: Mostrom, M. Mycotoxins. In: Peterson & Talcott Small Animal Toxicology (4th ed) . M. Romano ed. Submitted in 2023.
  • Type: Book Chapters Status: Submitted Year Published: 2023 Citation: Radke, SL, Mostrom M, Ensley SM, Fritz SA. Mycotoxins in Grains and Feeds, Ch 63. In: Diseases of Swine (11th ed). Submitted 2023.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Becker GM, Shira KA, Woods JL, Khilji SF, Schauer CS, Webb BT, Stewart WC, Murdock BM. Angular limb deformity associated with TSPAN18, NRG3 and NOVA2 in Rambouillet rams. Sci Rep 2023;13:16059.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Becker GM, Woods J, Schauer CS, Webb BT, Stewart WC, Murdoch BM. PSXIII-B-20 Variants within Genes Tspan18 and NRG3 Associated with Angular Limb Deformity in Rambouillet Rams. Journal of Animal Science 2023;100(S3):330.


Progress 10/01/21 to 09/30/22

Outputs
Target Audience:The target audience is practicing veterinarians, livestock producers, animal owners, and the general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The NDSU VDL continues to train a veterinary anatomic pathology resident, who is now in year two of a three-year residency. In addition, we hosted a veterinary student extern who has been accepted into a veterinary anatomic pathology residency program at a diagnostic laboratory and offered pathologist shadowing to pre-vet students throughout the summer. How have the results been disseminated to communities of interest?Results go out to clientele in the form of reports generated from samples submitted to the laboratory. On some occasions. NDSU VDL professionals will give presentations to veterinary clientele on disease trends seen in the region. A quarterly newsletter is published by the laboratory and distributed to practitioners across North Dakota and the region. What do you plan to do during the next reporting period to accomplish the goals?The VDL will continue its service to the state and region by testing samples, participating in NAHLN and Vet-LIRN, working closely with other state health agencies, and providing continuing education and outreach.

Impacts
What was accomplished under these goals? The laboratory processed nearly 12,500 accessions and performed approximately 40,000 tests last year including over 7000 tests for agents of public health significance (e.g., zoonotic agents, rabies). SARS-CoV-2 (COVID-19) testing on humans has ceased, but the NDSU VDL can be re-activated as needed by North Dakota Department of Public Health. In addition, as part of the NAHLN response to foreign animal disease emergence, we tested over 200 birds for high path avian influenza. Laboratory outreach included participating in the North Dakota Veterinary Medical Association annual meeting, preparing and distributing quarterly lab newsletters, writing articles for the North Dakota Stockman's Association, providing learning opportunities for undergraduate and graduate students, and organizing a continuing education day focused on ruminant nutrition, medicine, and parasitology for local veterinarians. New toxicological, microbiological, and molecular assays under development or validation include: Pasteurella multocida typing on MALDI-TOF Biotyper In collaboration with several other veterinary diagnostic laboratories, validation of new Brucella canis PCR method Bacterial whole genome sequencing of Salmonella in captive large felines and Pasteurella multocida in clinically ill calves SARS-CoV-2 detection and sequencing in companion animals Fungal PCR & metagenomic sequencing Cyanobacteria (blue-green algae) detection by mass spectrometry and sequencing Validation of the MagMAX CORE extraction kit, which uses fewer plates and allows reagents to be kept at room temperature Storage of dermatophyte/ringworm in PrimeStore Molecular Transport Media (MTM), which inactivates the organisms Validation of a commercially produced primer and probe cocktail for African Swine Fever Brain sodium method by ICP -OES Bromethelin or related metabolite desbromethalin in stomach contents or bait samples

Publications

  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Pecoraro HL, Stenger BS, Rice LE, Webb BT. Gross and histologic description of trematodosis in fetal and neonatal beef calves in North Dakota and Minnesota. J Vet Diagn Invest. 2022;34(5):870-873. doi: 10.1177/10406387221109619.
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Alvarez Narvaez S, Shen Z, Yan L, Stenger BLS, Goodman LB, Lim A, Nissly RH, Nair MS, Zhang S, Sanchez S. Optimized conditions for Listeria, Salmonella and Escherichia whole genome sequencing using the Illumina iSeq100 platform with point-and-click bioinformatic analysis. PLoS One. 2022;17(11):e0277659. doi: 10.1371/journal.pone.0277659.
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Lake K, Rankin S, Rosenkrantz W, Lakshmi S, Jacob M, Diaz Campos D, Maddock K, Cole S. In vitro efficacy of 0.2% and 0.4% sodium oxychlorosene against meticillin-resistant Staphylococcus pseudintermedius. Vet Dermatol. 2022. doi: 10.1111/vde.13121. Online ahead of print.


Progress 10/01/20 to 09/30/21

Outputs
Target Audience:The target audience is practicing veterinarians, livestock producers, animal owners, and the general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The first veterinary anatomic pathology resident began training August 2021. How have the results been disseminated to communities of interest?Results go out to clientele in the form of reports generated from samples submitted to the laboratory. On some occasions VDL professionals will give presentations to veterinary clientele on disease trends seen in the region. A quarterly newsletter is published by the laboratory and distributed to practitioners across North Dakota and the region. What do you plan to do during the next reporting period to accomplish the goals?The VDL will continue its service to the state and region by testing samples, participating in NAHLN, Vet-LIRN, working closely with other state health agencies and providing continuing education and outreach.

Impacts
What was accomplished under these goals? The laboratory processed over 12,900 accessions and performed more than 110,000 tests last year including over 10,000 tests for agents of public health significance (zoonotic agents, rabies, SARS-CoV-2) as well as tests for foreign animal disease through its participation in the NAHLN. Laboratory outreach included participating in the North Dakota Veterinary Medical Association annual meetings, quarterly lab newsletters, and articles for the North Dakota Stockman's Association. New toxicological, microbiological, and molecular assays under development or validation include: Phytoestrogen feed/forage screen by GC/MS Trace mineral liver biopsy method by ICP -MS and serum trace minerals by ICP-MS Brain sodium method by ICP -OES Bromethelin or related metabolite desbromethalin in stomach contents or bait samples Salmonella and Pasteurella multocida isolate analysis using ClinProTools software for advanced analysis of spectra from the MALDI-TOF P. multocida typing on MALDI-TOF Biotyper Brucella canis PCR Liver fluke PCR Bacterial whole genome sequencing Targeted fungal PCR & metagenomic sequencing SARS-CoV-2 and next-generation sequencing in companion animal samples

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Tyson GH, Ceric O, Guag J, [&], Webb B, et al. Genomics accurately predicts antimicrobial resistance in Staphylococcus pseudintermedius collected as part of Vet-LIRN resistance monitoring. Veterinary Microbiology 2021;254:109006.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Tkachenko A, Benson K, Mostrom M, Guag J, Reimschuessel R, Webb B. Extensive Evaluation via Blinded Testing of an UHPLC-MS/MS Method for Quantitation of Ten Ergot Alkaloids in Rye and Wheat Grains. J AOAC Int. 2021;104(3):546-554.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Williams LBA, Edmonds SE, Kerr SR, Broughton-Neiswanger LE, Snekvik KR.Clinical and pathologic findings in an outbreak in rabbits of natural infection by rabbit hemorrhagic disease virus 2 in the northwestern United States. J Vet Diagn Invest 2021;33(4):732-735.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Sohrabi M, Pecoraro HL, Combs CK. Gut Inflammation Induced by Dextran Sulfate Sodium Exacerbates Amyloid-beta Plaque Deposition in the AppNL-G-F Mouse Model of Alzheimer's Disease. J Alzheimers Dis 2021;79(3):1235-1255.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Casselli T, Divan A, Vomhof-DeKrey EE, Tourand Y, Pecoraro HL, Brissette CA. A murine model of Lyme disease demonstrates that Borrelia burgdorferi colonizes the dura mater and induces inflammation in the central nervous system. PLoS Pathog 2021;17(2):e1009256.
  • Type: Journal Articles Status: Under Review Year Published: 2022 Citation: Pecoraro HL, Stenger BS, Rice LE, Webb BT. Gross and histopathologic description of fetal and neonatal beef calf distomiasis in the upper midwestern United States
  • Type: Journal Articles Status: Submitted Year Published: 2022 Citation: Lake K, Rankin S, Rosenkrantz W, Lakshmi S, Jacob M, Diaz Campos D, Maddock K, Cole S. In vitro efficacy of sodium oxychlorosene against methicillin-resistant Staphylococcus pseudintermedius.