Use of Rapid Detection Methods of Pathogens Causing Diseases in Catfish in Natural Lakes in Alabama State

Project Director
Mohamed, AB, .

Recipient Organization
TUSKEGEE UNIVERSITY
(N/A)
TUSKEGEE,AL 36088

Performing Department
Microbiology

Non Technical Summary
Alabama is the second catfish producer nationwide. The three most important bacterial pathogen that cause great economical losses are Edwarsiella spp., Aeromonas spp. and Streptococcus iniae. Edwarsiella tarda and Streptococcus iniaeare also zoonotoc pathogen. For this year we will isolate identify conventionally and molecularly Edwardsiella spp. and Aeromonas spp. The antibiotic sensitivity pattern will be studied for the antibiotics used for control. Stock cultures from detected wild isoplates will be stored in in deep freeze till further use (phylogenetic analysis, vaccine development)

Animal Health Component

100%

Research Effort Categories

Basic

30%

Applied

40%

Developmental

30%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	0210	1100	100%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
0210 - Water resources;

Field Of Science
1100 - Bacteriology;

Keywords

edwarsiella tarda

aeromonas hydrophila

acquaculture

Goals / Objectives
The major Goal of this project is to decrease incidences of of bacterial diseases in acquaculture industry in Alabama State.This goal can be achieved as follows:1- Collect water samples from study area and tested for presence of fish disease causing pathoges (Edwarsiella spp and Aeromonas spp.)2- Develop Rapid molecular techniques validate and use3- Identify the antibiotic resistance pattern of disease causing pathogen in the study area

Project Methods
1- Water samples will be collected in 50 ml sterile tubes, from natural waters in the study area, (Macon, Russell, Montgomery and Bullock counties ) and transported to the laboratory in cool ice boxes.2- Centrifuge and incubate pallet in enrichment media3- Molecular testing will be initiated before enrichment step and after4- Molecular results will be compared with conventional results5- Biolog identification system will be used to confirm conventional result6- Antibiotic testing for detected wild pathogens7- DNA will be extracted from detected pathogens8- Stock cultures from detected isolates will be kept in brain heart infusion broth with 15% glycerine and store at -20 celsius degree till future use (Phylogenetic analysis).

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:1- Veterinary students in the College of Veterinary Medicine, Tuskegee University 2- Graduate students in the College of Veterinary Medicine Changes/Problems:Covid-19 curbed the process of isolating more wild Edwardsiella isolates to test the molecular test and assess the antibiogram of wild isolates. We shift the research to develop selective sensitive Edwarsiella broth culture medium. We are using the limited wild isolates isolated previously together with known ATCC Edwardsiella isolates in developing this medium What opportunities for training and professional development has the project provided?Two graduate students were trainedfor sample collection, isolation of Edwardsiella tarda using conventional culture isolation. How have the results been disseminated to communities of interest?One seminar was presented to veterinary students, graduate students and faculty in college of veterinary medicine. Knowledge regarding disease causing fish pathogens was dissemntaed during the seminar. Students and faculty shared scientific knowledge of the project about the developed selective sensitive broth culture medium for Edwardsiella What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Due to COVID-19 situation limited water samples were investigated. No enough wild Edwardsiella isolate were isolated to test the molecular test and antibiotic resistance pattern.

Publications