Source: VIRGINIA POLYTECHNIC INSTITUTE submitted to NRP
GEOGRAPHICAL VARIATION OF A WIDESPREAD AND EXPANDING INVASIVE GRASS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1016048
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2018
Project End Date
Jun 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
VIRGINIA POLYTECHNIC INSTITUTE
(N/A)
BLACKSBURG,VA 24061
Performing Department
School of Plant and Environmental Sciences
Non Technical Summary
Weeds continue to be the most costly and labor-intensive management challenge for agricultural producers globally. Weeds reduce crop productivity primarily by direct competition for growth-limiting resources, but may also serve as a host or reservoir for pathogens and pests, particularly in cases of weeds closely related to crops. Despite tremendous advances in crop development, herbicide chemistry and precision application, and alternative management technologies (e.g., flame weeders), both small and large-scale growers continue to face weed management challenges. Widespread adoption of herbicide resistant crops has been associated with a dramatic increase in herbicide resistant weeds (Heap 2018), forcing growers to apply additional herbicides or resort to mechanical or manual removal (Vencill et al. 2012). This has led to increased farm costs and unintended environmental impacts as less-safe chemistries are applied to address herbicide resistant weeds.Understanding the processes and limitations to how invasive and agricultural weeds spread can be used to mitigate future invasions,is especially important as climate change alters weed distributions (Dukes & Mooney 1999). Weeds continue to be the primary threat to agricultural yield, and invasive plants damage native ecosystems, therein reducing ecosystem functioning. Thus, we strive to understand what underlies the spread of invasive weeds to protect our human and natural systems.Johnsongrass (JG, Sorghum halepense) has the rare distinction of being both an invasive species and one of the world's worst agricultural weeds (Holm et al. 1997) [herein we use weed in the agricultural context, and invasive in the context of natural or minimally managed habitats]. JG costs US farmers tens of millions of dollars annually in management costs and yield losses (Burke, Wilcut & Cranmer 2006). Johnsongrass is a globally distributed, highly damaging weed and invader of natural areas (Warwick, Phillips & Andrews 1986), whose range is projected to expand northward with a warming climate (McDonald et al. 2009). With 26 herbicide-resistant biotypes now known (Heap 2018) it will only become more problematic in the future. For example, a glyphosate-resistant JG biotype discovered in Argentina in 2002 had covered 10,000 ha by 2009 (Binimelis, Pengue & Monterroso 2009). Listed as a noxious weed in 20 U.S. states, JG is also labeled an invasive species in 16 states (Quinn et al. 2013). This tremendous 'success' of JG can be partially attributed to its "invasive syndrome", with a complement of advantageous traits: robust spreading rhizomes, shattering inflorescences, rapid growth, seed dormancy, large and extensive annual seed production, tremendous disturbance tolerance, allelochemicals, and associations with nitrogen-fixing bacterial endophytes (Monaghan 1979; Paterson et al. 1995; Rout & Chrzanowski 2009; Liu et al. 2011).Our group has already generated a wealth of publicly available genetic and ecological data using microsatellite markers. We identified genetic clusters to reconstruct the expansion of founding populations (Sezen et al. 2016), which are centered in the Southeastern US. Across the introduced range of the US, JG exhibits extremely high genetic (Sezen et al. 2016) and phenotypic variability (Atwater et al. 2016b) and some level of habitat specialization (Atwater et al. 2016a). Importantly, our recent evidence suggests that the climate niche of edge populations is considerably wider than that of core (i.e., near founding locations) populations (Atwater et al. 2016b). Additionally, others have shown that JG lacks winter rhizome survival in some northern locations (Warwick, Phillips & Andrews 1986), thus exhibiting an annual life history. Finally, we have demonstrated that JG has differentiated in response to both climatic variation and habitat. Thus, there is evidence that edge populations are differentiating from core populations, and that climate may be playing an important role in limiting their range. While our previous work reveals the existence of considerable genetic and phenotypic differentiation in JG, it was not designed to thoroughly sample the edge and expanding front, nor to parse the ecological and genetic factors influencing range expansion in the species.
Animal Health Component
10%
Research Effort Categories
Basic
90%
Applied
10%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2062300106090%
2132300107010%
Goals / Objectives
Objectives1) To understand if Johnsongrass performs best when grown where it originated.2) To determine if Johnsongrass changes how it allocates resources in response to climate and competition.3) To elucidate whether Johnsongrass alters its physiology, photosynthetic rates, and rhizome growth in response to cold and dry growing conditions.
Project Methods
ProceduresThe goal of this study is to characterize the nature and mechanisms of range limits in a globally important invasive plant, and to identify the extent of life history trade-offs, and local adaptation among core, edge, and peripheral populations. We propose both field and growth chamber experiments to address these questions. The field experiment will allow us to assess Johnsongrass survival and growth in ecologically realistic conditions across both temperature and precipitation gradients, directly addressing Objectives 1 and 2. The growth chamber experiments will allow us to isolate the effects of important climatic drivers, from the field experiment (e.g., cold), on competition, and performance, which will be used to directly address Objective 3.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience: This project is designed to inform researchers, weed managers, farmers, students, and fellow scientists/faculty in providing important information on how weeds adapt to environmental stress. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has involved undergraduate and graduate students in achieving the project's objectives. They have been involved with setting up the experiments, collecting and analyzing data, and preparing products. How have the results been disseminated to communities of interest?Some results were presented at a scientific conference in early 2020 at the Northeastern Plant, Pest, and Soils Conference, as well as one peer-reviewed publication. What do you plan to do during the next reporting period to accomplish the goals?We will continue moving forward with the project as planned.

Impacts
What was accomplished under these goals? We are making appropriate progress on all of the objectives, which are detailed below. Objective 1) A large amount of phenotypic, survival, and winterkill observations have been collected across a wide range of gardens. Collaborators have sent their data to us as well.This data is currently being analyzed. The data analysis will continue with the goal of generating a peer-reviewed publication. This will provide insight into whether Johnsongrass does indeed perform better where it originated than populations from other locations. Objective2) The data collected above will also inform the role that climate and competition play in the performance and reproduction of Johnsongrass. This is also complemented by a greenhouse study that was completed and published that focused on photosynthetic variation. Below is a quotefrom the abstract that summarizes the major findings of this study: "We evaluated photosynthetic trait variation among populations, ecotypes, and home climates (i.e. the climates from the locations they were collected) of the widespread and expanding invader Johnsongrass (Sorghum halepense). We found that populations vary in the maximum net photosynthetic flux and the light-saturated net photosynthetic rate and that agricultural and non-agricultural ecotypes vary in apparent quantum yield and water-use efficiency (WUE). We also found that populations from warmer home climates had lower dark respiration rates, light compensation points, and WUEs. As Johnsongrass expands across the USA the abiotic and biotic environments are driving variation in its genetics, phenotypes, and its underlying physiology. Our study demonstrates the importance of evaluating physiological traits in invasive plants, especially as they relate to home climates." Objective 3) We are making appropriate progress on this objective. This will largely be achieved through studies in controlled environment conditions. We conducted one experiment evaluating the photosynthetic differences among a range of Johnsongrass populations. This data was published. Other experiments have been planned, with one completed.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Fletcher RA, Atwater DZ, Haak DC, Barney JN (2020) Ecological and evolutionary dynamics at the range edge of an expanding invasive species. Northeastern Plant, Pest, and Soil Conference. Philadelphia, PA
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kelly S, Fletcher RA, Barney JN (2020) Intraspecific, ecotypic, and home climate variation in photosynthetic traits of a widespread invasive grass. AoB Plants 12: plaa015 (https://doi.org/10.1093/aobpla/plaa015)


Progress 10/01/18 to 09/30/19

Outputs
Target Audience: This project is designed to inform researchers, weed managers, and farmers in providing important information on how weeds adapt to environmental stress. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This work includes training of undergraduate and graduate students who are involved with most aspects of the project. They assist with experimental setup, data collection and analysis, and will participate in presenting the work at various outlets. How have the results been disseminated to communities of interest?One poster was presented on some preliminary data at the Ecological Society of America in August, 2019 in Louisville, KY. This is the largest gathering of ecologists globally, sharing outputs with researchers from within and outside the US. What do you plan to do during the next reporting period to accomplish the goals?We will continue our work on schedule and foresee no issues in making appropriate progress on all objectives.

Impacts
What was accomplished under these goals? We are making appropriate progress on all three objectives. The field work has been in full swing, and will generate a huge amount of information on whether local Johnsongrass populations performbetter than those from other locations (Objective 1). A wide range of phenotypic and performance data has been collected across a range of Johnsongrass populations grown in a range of common gardens across its introduced range in the US. This data will also be used to inform how local climate shapes Johnsongrass performance, while our competitive treatment design will inform the role that interspecific competition shapes performance (Objective 2). We are on track to meet these objectives. We are also making progress on Objective 3 that will be informed by a series of studies in controlled environmental conditions.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Fletcher RA, Atwater DZ, Haak DC, Barney JN (2019) Evidence of an adaptive trade-off in a widespread and expanding invasive grass at the edge of its distribution. Ecological Society of America, Louisville, KY


Progress 07/01/18 to 09/30/18

Outputs
Target Audience: This project is designed to inform researchers, weed managers, and farmers in providing important information on how weeds adapt to environmental stress. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Currently two graduate students are working on this project with undergraduate students joining in the future. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We will comtinue collecting and analyzing the datasets from the common gardens, which we anticipate to occur over the next reporting period. We also hope to begin working on objective 3.

Impacts
What was accomplished under these goals? The common garden has been established in the five locations throughout the U.S. with partners. We continue to collect data on this large experiment. We are only at the very beginning stages of this project, but are well on track to make headway on the objectves, particularly on objectives 1 and 2.

Publications