Source: NORTHERN ARIZONA UNIVERSITY submitted to
POLLINATOR RESPONSE TO RESTORATION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1014923
Grant No.
2018-67013-27539
Project No.
ARZW-2017-06564
Proposal No.
2017-06564
Multistate No.
(N/A)
Program Code
A1113
Project Start Date
Feb 1, 2018
Project End Date
Jan 31, 2023
Grant Year
2018
Project Director
Grady, K. C.
Recipient Organization
NORTHERN ARIZONA UNIVERSITY
(N/A)
FLAGSTAFF,AZ 86011
Performing Department
SCHOOL OF FORESTRY
Non Technical Summary
We propose to address critical knowledge gaps to support the development of pollinator-dependent plant materials for use in the rapidly growing"restoration economy," where agencies, nonprofits, and businesses collaborate to restore degraded ecosystems to promote agriculture, tourism, conservation, fire resilience, multi-use landscapes, and more.We propose the largest to-date provenance trial in the arid western US, including nearly 50,000 plants of 12 plant species to develop seed zone guidelines and predict plant-pollinator response to climate change. We will also initiate a regional seed production trial of 105,000 plants to spearhead a farm-based seed development program for pollinator plants across three states.
Animal Health Component
0%
Research Effort Categories
Basic
40%
Applied
60%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20507101070100%
Keywords
Goals / Objectives
We propose to address critical knowledge gaps to support the development of pollinator-dependent plant materials for use in the rapidly growing "restoration economy", where agencies, nonprofits, and businesses collaborate to restore degraded ecosystems to promote agriculture, tourism, conservation, fire resilience, and multi-use landscapes. We propose the largest provenance trial in the arid western US that includes nearly 50,000 plants of 12 plant species to develop seed zone guidelines and predict plant-pollinator response to climate change. We will also initiate a regional seed production trial of 200,000 plants to spearhead a farm-based seed development program for pollinator plants across three states. Involving multiple stakeholders, this project will result in data-driven best practice guidelines for selection, combination, and cultivation of plants and provenances for large-scale restoration seed production among agriculturalists working to supply restoration professionals with needed forb seed to promote pollinator communities.?
Project Methods
This project consists of two main objectives: A) a large-scale test of seed zone suitability and local adaptation/maladaptation for 12 pollinator-dependent perennial forb species that are critical for restoration. In this test, we will quantify pollinator communities interacting with 21 separate plant populations per species at four common gardens - populations and gardens vary by climate and spatial distance from one another; and B) a seed lot production test, where we will focus on development of seed production strategies for promising foundation species (those that support diverse pollinator communities and produce ample seeds that can increase seed supply for large-scale triage-type restoration). These strategies include examining population-level variation in seed production as impacted by different watering regimes and planting densities. For density treatments, we are interested in minimizing competition among plant neighbors while maximizing seed production on an areal basis. For the watering treatment, we are interested in minimizing water use while maximizing seed yield. We will also manipulate pollinator nesting resources alongside different planting strategies to maximize seed set by increasing diversity of pollinator communities.

Progress 02/01/18 to 01/31/23

Outputs
Target Audience:Our major common garden structure and seed increase studies includes partnerships with the US Forest Service, the Bureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agricultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively disseminated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Research website at https://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use- growing-restoration-economy. In addition, our target audience includes training of graduate students, undergraduate students, K-12 students, and Navajo youth ages 18-25. We have graduated three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) and one PhD student is still (Audrey Harvey) involved in the project. Data collection by MS students has been completed and theses were successfully defended. Harvey is still working on data collection and dissertation preparation. In addition, we have employed over 13 undergraduate students to help with data collection. We have also worked with K-12 students from the Flagstaff School District to teach them about plant-pollinator interactions through our EcoKids program. Our Restoration Certificate Program for tribal youth was delivered to a total of 27 youth in the duration of this grant and will continue. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We trained four graduate students, over 20 undergraduate students, more than 300 K-12 students, and 27 Navajo youth ages 18-25. We have three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) and one PhD student (Audrey Harvey) involved in the project. Data collection by MS students has been completed and theses were successfully defended. Harvey is still working on data collection and dissertation preparation. In addition, we have employed over 20 undergraduate students to help with data collection. We have also worked with 300+ K-12 students from the Flagstaff School District to teach them about plant-pollinator interactions through our EcoKids program. Our Ancestral Lands Restoration Certificate Program for tribal youth was delivered to 27 youth and is now a permanent part of the conservation corps youth program. This program is continuing this year and into the future, in part, due to the research related to this project. How have the results been disseminated to communities of interest?Our major common garden structure and seed increase studies includes partnerships with the US Forest Service, the Bureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agricultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively disseminated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Research website at https://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use- growing-restoration-economy. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? In previous reporting periods, we hand-collected seed from wild populations of 25 species of native forbs in the southwest in nearly 300 locations; we conducted all the logistical legwork (permitting, permissions, etc) to establish four common garden locations in Arizona and two seed production experiments in Utah and New Mexico. In sum, we have planted over 25,000 plants as part of this research. Common garden trials include a gradient from warm sites to cooler sites and are located in the Sierra Ancha mountains and in Flagstaff, Arizona. Research for the current year included repeat measurements of physiological traits and pollinator communities from common gardens. It also included examination of plant traits, seed production, and maternal effects at outplanting seed production farms in Utah and New Mexico. These gardens will continue to be used in the future as they are now a core component of educational curriculum design to use them. In addition, seed production trials have resulted in major efforts to increase seed production in the region. This includes current plans to work with Navajo Agricultural Production Industries and communities throughout the region. Seed production initiatives are underway in Flagstaff, Tuba City, Winslow, and Leuppe. Our submitted manuscript (Aslan, C., K. Haubensak and K. Grady) "Effective and feasible mechanisms to support native invertebrate pollinators in agricultural landscapes: A meta-analysis" was accepted and published in Ecosphere. Other manuscripts are taking longer than expected to be published; however, we will continue to push manuscripts through the peer-review process with the expectation of over 10 publications from this research.

Publications


    Progress 02/01/21 to 01/31/22

    Outputs
    Target Audience:Our major common garden structure and seed increase studies includes partnerships with the US Forest Service, theBureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agricultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively disseminated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Research website athttps://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy. In addition, our target audience includes training of graduate students, undergraduate students, K-12 students, and Navajo youth ages 18-25. We have three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) andone PhD student (Audrey Harvey)involved in the project. Data collection by MS students has been completed and theses were successfully defended this year. Harvey is still working on data collection and dissertation preparation. In addition, we have employed over 6 undergraduate students to help with data collection. We have also worked with K-12 students from the Flagstaff School District to teach them about plant-pollinator interactions through our EcoKids program. Our Restoration Certificate Program for tribal youth was delivered to five youth this year.? Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We trainedgraduate students, undergraduate students, K-12 students, and Navajo youth ages 18-25. We have three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) andone PhD student (Audrey Harvey)involved in the project. Data collection by MS students has been completed and theses were successfully defended this year. Harvey is still working on data collection and dissertation preparation. In addition, we have employed over 6 undergraduate students to help with data collection. We have also worked with K-12 students from the Flagstaff School District to teach them about plant-pollinator interactions through our EcoKids program. Our Restoration Certificate Program for tribal youth was delivered to five youth this year. How have the results been disseminated to communities of interest?Our major common garden structure and seed increase studies includes partnerships with the US Forest Service, theBureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agricultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively disseminated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Research website athttps://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy.? What do you plan to do during the next reporting period to accomplish the goals?The next reporting period will include submitting dissertation of one PhD student. This will include analysis of data and manuscript preparation for field trials of seed production. We also plan to submit above-mentioned manuscripts. Field data will be collected from field sites related to pathogens and insects that we have started to see as problematic in farming trials. We will also disseminate our results widely to agencies interested in seed increase for restoration.

    Impacts
    What was accomplished under these goals? In previous reporting periods, we hand-collected seed from wild populations of 25 species of native forbs in the southwest in nearly 300 locations; we conducted all the logistical legwork (permitting, permissions, etc) to establish four common garden locations in Arizona and two seed production experiments in Utah and New Mexico. In sum, we have planted over 25,000 plants as part of this research. Common garden trials include a gradient from warm sites to cooler sites and are located in the Sierra Ancha mountains and in Flagstaff, Arizona. Research for the current year included repeat measurements of physiological traits and pollinator communities from common gardens. It also included examination of plant traits, seed production, and maternal effects at outplanting seed production farms in Utah and New Mexico. Our submitted manuscript (Aslan, C., K. Haubensak and K. Grady) "Effective and feasible mechanisms to support native invertebrate pollinators in agricultural landscapes: A meta-analysis" was accepted and published in Ecosphere. Two manuscripts have been prepared and will be submitted in the next reporting period: 1) Variation in flowering phenology and traits of pollinator-dependent forbs used in restoration; 2) Linking differentiation of plant functional traits to source climate in key native restoration species.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2022 Citation: Aslan, CE, Haubensak, KA, and KC Grady (2022) Effective and feasible mechanisms to support native invertebrate pollinators in agricultural landscapes: A meta-analysis. Ecosphere, 13, e3982
    • Type: Theses/Dissertations Status: Published Year Published: 2021 Citation: Lee, Hannah (2021) Linking differentiation of plant functional traits to source climate in key native restoration species. MS Thesis. Northern Arizona University.
    • Type: Theses/Dissertations Status: Published Year Published: 2021 Citation: Bauer, Tatia (2021) Variation in flowering phenology and traits of pollinator-dependent forbs used in restoration. MS Thesis. Northern Arizona University.


    Progress 02/01/20 to 01/31/21

    Outputs
    Target Audience:Our major common garden structure and seed increase studies now include partnerships with the US Forest Service, theBureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agicultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively dissemnated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Reseach website athttps://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy. In addition, our target audience includes training of graduate students, undergraduate students, K-12 students, and Navajo youth ages 18-25. We have three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) andone PhD student (Audrey Harvey)involved in the project. Data collection by all students has been completed and theses, dissertation, and publications are in progress. In addition, we have employed over 12 undergraduate students to help with data collection. We have also worked with students from the Flagstaff School District to teach them about plant-pollinator interactions through a newly developed EcoKids program. We have also worked with the Ancestral Lands Conservation Corps with five Navajo youth to conduct measurements related toplant-pollinator interactions. This is part of a newly created Restoration Certificate Program for tribal youth. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have providedtraining to graduate students, undergraduate students, K-12 students, and Navajo youth ages 18-25. We have three MS students (Hannah Lee, Tatia Bauer, and Kaci Fankhauser) andone PhD student (Audrey Harvey)involved in the project. Data collection by all students has been completed and theses, dissertation, and publications are in progress. In addition, we have employed over 12 undergraduate students to help with data collection. We have also worked with students from the Flagstaff School District to teach them about plant-pollinator interactions through a newly developed EcoKids program. We have also worked with the Ancestral Lands Conservation Corps with five Navajo youth to conduct measurements related toplant-pollinator interactions. This is part of a newly created Restoration Certificate Program for tribal youth. How have the results been disseminated to communities of interest?Our major common garden structure and seed increase studies now include partnerships with the US Forest Service, theBureau of Land Management, Navajo Agricultural Production Industries, University of Utah Rio Mesa Research Station, and New Mexico State University Agicultural Extension. Provenance trials and seed increase studies were developed and implemented on lands managed by these organizations. Results of studies are actively dissemnated to these partners and will help guide restoration procedures with all organizations. Our research is highlighted in the US Forest Service Rocky Mountain Reseach website athttps://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy.? What do you plan to do during the next reporting period to accomplish the goals?The next reporting period will include submitting theses of three MS students. This will include analysis of data collected up to this point and will be the jumping off point for the development of manuscripts for publication. Once this has occurred, we will be ready to provide seed source guidelines for our region based on both biotic and abiotic factors - i.e., promotion of pollinators and plant functional aspects. This will be a novel contribution to the seed provisioning literature. We will also start work on physiological attributes of our outplantings for seed increase on farms in New Mexico and Utah.

    Impacts
    What was accomplished under these goals? In the previous reporting periods, we hand-collected seed from wild populations of 25 species of native forbs in the southwest in nearly 300 locations; we conducted all the logistical legwork (permitting, permissions, etc) to establish four common garden locations in Arizona and two seed production experiments in Utah and New Mexico. In sum, we have planted over 25,000 plants as part of this research. Common garden trials include a gradient from warm sites to cooler sites and are located in the Sierra Ancha mountains and in Flagstaff, Arizona. Research for the current year included detailed plant physiological measurements on a subset of plants at multiple sites and identification of pollinators using these plants. Physiological measurements such as growth rate, flower and foliar phenology, specific leaf area, and foliar nutrient content were examined and compared among populations and garden sites to evaluate genetic variation in relationship to climate. Such information is being used to develop seed transfer guidelines for pollinator species. Identification of over 100 species of bees has been incorporated into models of how climate change and assisted migration may impact plant-pollinator interactions. We have also prepared a meta-analysis for publication (Aslan, C., K. Haubensak and K. Grady) "Effective and feasible mechanisms to support native invertebrate pollinators in agricultural landscapes: A meta-analysis" that was submitted for publication in Ecosphere. In addition, this year we initiated seed production trials in New Mexico and Utah with farmers interested to produce and market seeds for use in restoration. This included collecting seeds from installed provenance trials and growing them out in production trials at two farms. Survival data from the first year of planting was collected.

    Publications


      Progress 02/01/19 to 01/31/20

      Outputs
      Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?During this reporting period, we have supported and trained three MS graduate students on the project: Hannah Lee, Tatia Bauer, and Kaci Fankhauser. Lee and Bauer are focused on patterns of local adaptation across the focal plant species and pollinator response to those patterns; Fankhauser is focused on patterns of herbivory by vertebrates in our common gardens. We have additionally been mentoring and training two undergraduate students: Isabella Johnson and Billie Fraser. Johnson and Fraser have been examining physical and biological differences in soils across the common garden locations; they have recently been awarded an undergraduate research grant by Northern Arizona University to continue this work. How have the results been disseminated to communities of interest?Our project continues to be highlighted by the US Forest Service on the Sierra Ancha Experimental Forest website: https://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy. Lee and Bauer presented the overarching project and preliminary data at the 15th Biennial Conference of Science & Management on the Colorado Plateau & Southwest Region (September 2019, Flagstaff, AZ): Developing pollinator-dependent plant materials for use in a growing restoration economy. This presentation was also posted on the Colorado Plateau Native Plant Program website: http://www.landscope.org/CPNPP/News/. What do you plan to do during the next reporting period to accomplish the goals?We will continue to actively collect data on plant health, performance, and interactions with pollinators in all common gardens during the upcoming summer 2020 field season. Additionally, we will fund an incoming Ph.D. student, Audrey Harvey, to help coordinate our work with commercial growers on trial plantings of seed mixes determined by our year 1 preliminary data. Harvey will help establish trials at three locations in New Mexico, Utah, and Nevada.

      Impacts
      What was accomplished under these goals? In the previous reporting period we hand-collected seed from wild populations of 17 species of native forbs in the southwest in nearly 300 locations; we conducted all the logistical legwork (permitting, permissions, etc) to establish five common garden locations in Arizona and Nevada. Beginning in this reporting period, our team spent 480 hours in the greenhouse to clean and pre-treat all seed then plant and maintain seedlings in the greenhouse; beginning in May our team outplanted nearly 20,000 forbs across the five field sites. We estimate 1350 person hours to build and plant all five common garden sites. Additionally, we have spent an estimated 410 hours for watering, weeding, maintenance and data collection since garden establishment. During the 2019 summer season, our team conducted basic maintenance for survival (weeding and watering). The team also began data collection on plant heights and survival, flowering phenology, and pollinator visitation. These initial data from at least two of the gardens are currently being prepared for publication (Grady, K., C. Aslan, H. Lee, T. Bauer, and K. Haubensak. In prep. Patterns of intraspecific variation and local adaptation in pollinator-enhancing native forb species of the southwestern US). We have also prepared a meta-analysis for publication (Aslan, C., K. Haubensak and K. Grady. In prep. The effectiveness of practical strategies to boost native pollinator communities in agricultural contexts). Those data are crucial to inform the next phase of work, slated to occur in 2021, when we will work with farmers to trial production of these plants in farm environments.

      Publications

      • Type: Journal Articles Status: Other Year Published: 2021 Citation: Aslan, C., K. Haubensak and K. Grady. In prep. The effectiveness of practical strategies to boost native pollinator communities in agricultural contexts.
      • Type: Journal Articles Status: Other Year Published: 2021 Citation: Grady, K., C. Aslan, H. Lee, T. Bauer, and K. Haubensak. In prep. Patterns of intraspecific variation and local adaptation in pollinator-enhancing native forb species
      • Type: Journal Articles Status: Other Year Published: 2021 Citation: Grady, K., C. Aslan, H. Lee, T. Bauer, and K. Haubensak. In prep. Locally adapted populations of invasive species support highest abundance of pollinators: implications for native forbs in the southwest.


      Progress 02/01/18 to 01/31/19

      Outputs
      Target Audience:The first year of the project is not about reaching target audiences. We therefore have nothing to report. Changes/Problems:One change that we have made includes developing a watering/drought experiment to closely control the impact of different climatic conditions on plant-pollinator interactions. In both Sierra Ancha at a hot site at 5,000 feet elevation and at Flagstaff a cold site at 7,000 feet elevation, we will plant 8 populations (4 from hot areas, 4 from cooler areas)from 10 species into the following treatments: irrigated with supplemental water; control; rain-out shelter removing 50% of annual precipitation. This experiment will be useful for directly examing climate change impacts at two locations rather than only relying on the translocation experiment to examine climate change or assisted migration. This additional experiment does not require additional funding. What opportunities for training and professional development has the project provided?We have recruited several students to assist with this project, providing them training in native plant identification and propagation and in plant performance data collection. This has included three MS students, each of whom is assisting in the preparation and planting of the gardens and will perform their MS research on discrete aspects of this work. One of these students, Kaci Fankhauser, has been recruited in part to assist with outreach to classrooms and the general public about native plant restoration and propagation. How have the results been disseminated to communities of interest?This project has been described by the US Forest Service on the Sierra Ancha Experimental Forest website: https://www.fs.fed.us/rmrs/projects/developing-pollinator-dependent-plant-materials-use-growing-restoration-economy. As the gardens become established, we will contribute photos and additional information to that description, so that it can be an updated source of interpretation and outreach to the public. Co-PI Aslan delivered an interview about the research to the Flagstaff NPR affiliate, KNAU, and that interview was broadcast in locations throughout the state. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, all of our common gardens will be established and we will be actively recording data on plant health, performance, and interactions with pollinators. We will evaluate which plant species, provenances, and moisture conditions are linked to best performance and most diverse pollinator interactions across the elevational and latitudinal range of our study. By the end of the next reporting period, we will have selected the subset of species that best serve as foundational species, to work with commercial growers on trial plantings of the resulting seed mix.

      Impacts
      What was accomplished under these goals? See "Activities" above: we are in the first phase of the project where we have successfully collectedseeds from between 12 and 36 populations from24 native plant species and propagated them in the greenhouse; we will be outplanting them to the provenance trial sites (total of 6 gardens) in May, June and August, 2019. We have added a drought manipulation experiment to this set of trials to more clearly understand the role of drought in plant-pollinator interactions in this project. We have brought three graduate students (see below) onto the project whose theses will be directly developed from this project.

      Publications