Source: AGRICULTURE, TENNESSEE DEPARTMENT OF submitted to
NAHLN
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1010502
Grant No.
2016-37620-25768
Project No.
TENW-2016-08612
Proposal No.
2016-08612
Multistate No.
(N/A)
Program Code
AA-G
Project Start Date
Sep 1, 2016
Project End Date
Aug 31, 2017
Grant Year
2016
Project Director
McLaughlin, B.
Recipient Organization
AGRICULTURE, TENNESSEE DEPARTMENT OF
ELLINGTON AGRICULTURAL CENTER
NASHVILLE,TN 37204-9029
Performing Department
Agriculture
Non Technical Summary
The Kord Animal Health Diagnostic Laboratory (KAHDL) currently offers limited molecular diagnostic testing beyond that related to our National Animal Health Laboratory Network (NAHLN) capability and capacity. We have seven staff members trained and proficiency tested for avian influenza (AI) PCR testing, and two for exotic Newcastle disease (END) PCR testing, classic swine fever (CSF) PCR testing, and foot and mouth disease (FMD) PCR testing. Six staff are proficiency tested for Pseudorabies testing by non-PCR methods and staff members in other sections evaluate specimens for surveillance and OIE Reportable Diseases. Within the molecular diagnostics section, the section supervisor and technicians (Microbiologist 2 level) also have multiple other duties that support animal disease diagnostic testing associated with routine daily laboratory submissions. KAHDL is currently AAVLD accredited and including the Director has four well trained and experienced pathologists, including one pathologist with ACVP board certification. PCR testing for other diseases is very limited at this time.Under this NAHLN cooperative agreement proposal, grant funds would be used for purchase of equipment and reagents for implementation of PCR testing for several important diseases, including bovine viral diarrhea, Salmonella, equine adenovirus, equine viral arteritis, equine influenza, equine protozoal encephalomyelitis, Lawsonia, nocardioform abortion, Potomac horse fever, Rhodococcus equi, and canine influenza.Remaining funds are designated to be used for pathologist continuing education - particularly in the area of foreign and emerging diseases. Including the Laboratory Director, there are four pathologists; budgeted funding will cover registration and travel to one major meeting annually and may allow additional funds for other education materials.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31133991040100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3399 - Beef cattle, general/other;

Field Of Science
1040 - Molecular biology;
Goals / Objectives
The Kord Animal Health Diagnostic Laboratory (KAHDL) currently offers limited molecular diagnostic testing beyond that related to our National Animal Health Laboratory Network (NAHLN) capability and capacity. We have seven staff members trained and proficiency tested for avian influenza (AI) PCR testing, and two for exotic Newcastle disease (END) PCR testing, classic swine fever (CSF) PCR testing, and foot and mouth disease (FMD) PCR testing. Six staff are proficiency tested for Pseudorabies testing by non-PCR methods and staff members in other sections evaluate specimens for surveillance and OIE Reportable Diseases. Within the molecular diagnostics section, the section supervisor and technicians (Microbiologist 2 level) also have multiple other duties that support animal disease diagnostic testing associated with routine daily laboratory submissions. KAHDL is currently AAVLD accredited and including the Director has four well trained and experienced pathologists, including one pathologist with ACVP board certification. PCR testing for other diseases is very limited at this time.Under this NAHLN cooperative agreement proposal, grant funds would be used for purchase of equipment and reagents for implementation of PCR testing for several important diseases, including bovine viral diarrhea, Salmonella, equine adenovirus, equine viral arteritis, equine influenza, equine protozoal encephalomyelitis, Lawsonia, nocardioform abortion, Potomac horse fever, Rhodococcus equi, and canine influenza.Remaining funds are designated to be used for pathologist continuing education - particularly in the area of foreign and emerging diseases. Including the Laboratory Director, there are four pathologists; budgeted funding will cover registration and travel to one major meeting annually and may allow additional funds for other education materials.
Project Methods
Appropriate PCR-related equipment and reagents will be purchased in order to permit diagnosis of numerous animal diseases. Appropriate test validation, controls, training, and use of established and controlled protocols will be performed to ensure test accuracy and traceability.

Progress 09/01/16 to 08/31/17

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Two veterinary pathologists attended the annual meeting of the American Association of Veterinary pathologists, where they attained CE. The laboratory director attended the annual meeting of the American Association of Veterinary Laboratory Diagnosticians for CE and as a representative of the laboratory to NAHLN and AAVLD. How have the results been disseminated to communities of interest?Information regarding the availability of the new PCR tests has been disseminated to the laboratory's clientele via emails and website. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Under these goals, equipment and reagents were purchased and utilized to greatly enhance the laboratory's ability to diagnose important animal diseases, including zoonoses, via PCR testing. These diseases include: Canine influenza, subtype H3N8; Swine influenza; Eastern Equine encephalitis; Leptospirosis; West Nile Virus; Equine influenza; Equine herpesvirus-1, neurologic form; Equine herpesvirus-1, abortion form; Equine herpesvirus-4, respiratory form; Equine protozoal myeloencephalitis; and Clostridium difficile, toxins A and B. In addition, three professionals attended national scientific meetings for CE and/or representation of the laboratory at AAVLD and NAHLN meetings.

Publications