Source: UNIVERSITY OF ARIZONA submitted to
NANOTECHNOLOGY AND BIOSENSORS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1010010
Grant No.
(N/A)
Project No.
ARZT-1370140-R22-145
Proposal No.
(N/A)
Multistate No.
NC-_old1194
Program Code
(N/A)
Project Start Date
Oct 1, 2016
Project End Date
Sep 30, 2021
Grant Year
(N/A)
Project Director
Yoon, JE.
Recipient Organization
UNIVERSITY OF ARIZONA
888 N EUCLID AVE
TUCSON,AZ 85719-4824
Performing Department
Biosystems Engineering
Non Technical Summary
The resulting biosensors should be made fully handheld, all-in-one, andeasy-to-use, while generating the assay results in less than10 min.Liquid handling and samplepre-treatment should be minimized.
Animal Health Component
25%
Research Effort Categories
Basic
0%
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
4041430202025%
4043320202025%
7124010202025%
7124030202025%
Knowledge Area
712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins; 404 - Instrumentation and Control Systems;

Subject Of Investigation
3320 - Meat, beef cattle; 1430 - Greens and leafy vegetables; 4010 - Bacteria; 4030 - Viruses;

Field Of Science
2020 - Engineering;
Goals / Objectives
Develop devices and systems incorporating microfabrication and nanotechnology Develop a framework for economic, environmental and health risk assessment for nanotechnologies applied to food, agriculture and biological systems
Project Methods
Use microfabrication and 3D printingtechnology to create various lab-on-a-chip (LOC)devices, including paper microfluidics. Interface these LOCs with smartphone, utilizing its white LED flash, digital camera, internal sensors, GPS, etc. Develop software application. Use antibody-based immunoassays, nucleic acid-based polymerase chain reaction (PCR) assays, as well as spectroscopic analyses - ultraviolet (UV), visible, and near infrared (NIR).

Progress 10/01/16 to 09/30/21

Outputs
Target Audience:Academic research; environmental monitoring;food safety, veterinary diagnostics; medical diagnostics; tissue engineeing and emerging biotech industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?1 post-doctoral fellow, 11 graduate students, and 9 undergraduate students were mentored by the PI during the project period. How have the results been disseminated to communities of interest?1. Academic research: through disseminating our work in professional society's annual meetings (IBE, Biosensors World Congress, and ACS), as well as seminars in universities and governmental agencies (JPL-NASA). 2. Industry: through a series of discussion and seminars with industry. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Economic Value or Efficiency: Alternative method and device have been proposed and successfully demonstrated using of a smartphone-based fluorescence microscope to paper microfluidic devices, where the fluorescent particles were imaged and counted one-by-one. Through developing an original code now based on artificial intelligence, these counts were successfully related to the target presence and its concentration. A user-friendly prototype has been designed and fabricated, and successfully demonstrated for field water and human saliva samples. This method provides extremely low limit of detection, down to a single copy virus level or to sub-femtogram (fg) scale. Economic Value or Efficiency: The above method has also been demonstrated in quantifying cancer cells from human blood samples with excellent sensitivity and specificity, norovirus from wastewater, COVID-19 virus from aerosols, and COVID-19 virus from human saliva samples. Economic Value or Efficiency: A novel, paper-based organ-on-a-chip has been designed and fabricated, that is simpler and cheaper to fabricate, while allowing easy delivery of mechanical stimuli. This model has been demonstrated for blood vessel-on-a-chip. Environmental Quality: A novel Raspberry Pi based optics device has been designed, fabricated, and tested for classifying the oil types from oil spill samples from open sea water. Support vector machine (SVM) based machine learning algorithm has been developed and successfully classified the oil type with > 90% accuracy. Environmental Quality: These demonstrations can significantly improve the applicability of our biosensor methods and devices, which can be used by a lay person in myriads of environmental conditions. Such demonstrations will protect the general public from potential health risks fromfood, water, aerosols, and humans. Published 11peer-reviewed journal papers in the final period (10/1/2020-9/30/2021). This includes the paper published in Nature Protocols. The tissue engineering textbook is also in press. Also published 1 book chapter, made 7 conference presentations, and 6 invited talks (including the one from Jet Propulsion Laboratory). Received a new grant from ABOR-TRIF. The Tucson Water project was again renewed with additional grant support. Graduated 1PhD(Kaarj) and 4 MS's (Akarapipad, Zenhausern, Nguyen, and Zubler). Two of my former PhD trainees have secured tenure-track faculty positions - Kaarj at Mahidol University in Thailand and Chung at Seoul National University in South Korea.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Katelyn Sosnowski, Patarajarin Akarapipad and Jeong-Yeol Yoon, "The Future of Microbiome Analysis: Biosensor Methods for Big Data Collection and Clinical Diagnostics," Medical Devices & Sensors, 2020, 3(5): e10085.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Alanna V. Zubler and Jeong-Yeol Yoon, "Proximal Methods for Plant Stress Detection Using Optical Sensors and Machine Learning," Biosensors, 2020, 10(12): 193.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Ryan Zenhausern, Chia-Hung Chen, and Jeong-Yeol Yoon, "Microfluidic Sample Preparation for Respiratory Virus Detection: A Review," Biomicrofluidics, 2021, 15: 011503.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Soo Chung, Lane E. Breshears, Alana Gonzales, Christian M. Jennings, Christina M. Morrison, Walter Q. Betancourt, Kelly A. Reynolds and Jeong-Yeol Yoon, "Norovirus Detection in Water Samples at the Level of Single Virus Copies per Microliter Using a Smartphone-based Fluorescence Microscope," Nature Protocols, 2021, 16(3): 1452-1475.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Eric S. McLamore, Evangelyn Alocilja, Carmen Gomes, Sundaram Gunasekaran, Daniel Jenkins, Shoumen P.A. Datta, Yanbin Li, Yu Mao, Sam R. Nugen, Jose I. Reyes-De-Corcuera, Paul Takhistov, Olga Tsyusko, Jarad P. Cochran, Tzuen-Rong Tzeng, Jeong-Yeol Yoon, Chenxu Yu, and Anhong Zhou, "FEAST of Biosensors: Food, Environmental and Agricultural Sensing Technologies (FEAST) in North America," Biosensors and Bioelectronics, 2021, 178: 113011.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Alexander S. Day, Tiffany-Heather Ulep, Babak Safavinia, Tyler Hertenstein, Elizabeth Budiman, Laurel Dieckhaus, and Jeong-Yeol Yoon, "Emulsion-based Isothermal Nucleic Acid Amplification for Rapid SARS-CoV-2 Detection via Angle-dependent Light Scatter Analysis," Biosensors and Bioelectronics, 2021, 179: 113099.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Sangsik Kim, Anakaren Romero-Lozano, Dong Soo Hwang, and Jeong-Yeol Yoon, "A Guanidinium-rich Polymer as a New Universal Bioreceptor for Multiplex Detection of Bacteria from Environmental Samples," Journal of Hazardous Materials, 2021, 413: 125338.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Yan Liang and Jeong-Yeol Yoon, "In Situ Sensors for Blood-Brain Barrier (BBB) on a Chip," Sensors and Actuators Reports, 2021, 3: 100031.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Patarajarin Akarapipad, Kattika Kaarj, Yan Liang, and Jeong-Yeol Yoon, "Environmental Toxicology Assays Using Organ-on-Chip," Annual Review of Analytical Chemistry, 2021, 14: 155-183.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Kenneth E. Schackart III and Jeong-Yeol Yoon, "Machine Learning Enhances the Performance of Bioreceptor-Free Biosensors," Sensors, 2021, 21(16): 5519.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Sosnowski, K., Loh, A., Kim, T. J., Shir, H., Zubler, A. V., Ha, S. Y., Kim, U. H., & Yoon, J. (2021, Apr.). Raspberry Pi based, UV-fluorescent spectrophotometer device for the analysis of oil types obtained from oil spills. 2021 Annual Conference of the Institute of Biological Engineering. Online: Institute of Biological Engineering (IBE).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Kim, S., Lee, M., Wiwasuku, T., Day, A. S., Youngme, S., Hwang, D. S., & Yoon, J. (2021, Apr.). Taste sensor inspired peptide-based bacterial identification on smartphone-based paper microfluidic device. 2021 Annual Conference of the Institute of Biological Engineering. Online: Institute of Biological Engineering (IBE).
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Sangsik Kim, Min Hee Lee, Theanchai Wiwasuku, Alexander S. Day, Sujittra Youngme, Dong Soo Hwang, and Jeong-Yeol Yoon, "Human Sensor-inspired Supervised Machine Learning of Smartphone-based Paper Microfluidic Analysis for Bacterial Species Classification," Biosensors and Bioelectronics, 2021, 188: 113335.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Day, A. S., Ulep, T., Safavinia, B., Hertenstein, T., Budiman, E., Dieckhaus, L., & Yoon, J. (2021, Apr.). Emulsion-based Isothermal Nucleic Acid Amplification for Rapid SARS-CoV-2 Detection via Angle-dependent Light Scatter Analysis. 2021 Annual Conference of the Institute of Biological Engineering. Online: Institute of Biological Engineering (IBE).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Sosnowski, K., Bills, M. V., Loh, A., Nguyen, B. T., Yim, U. H., & Yoon, J. (2021, July). Raspberry Pi based, UV-fluorescent spectrophotometer device for the analysis of oil types obtained from oil spills. 31st Anniversary World Congress on Biosensors. Online: Elsevier.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Kaarj, K., Akarapipad, P., Breshears, L. E., Sosnowski, K., Nguyen, B. T., Baker, J., Worobey, M., & Yoon, J. (2021, July). Rapid and sensitive detection of SARS-CoV-2 from human clinical samples using flow-based smartphone analysis on paper microfluidic chip. 31st Anniversary World Congress on Biosensors. Online: Elsevier.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Day, A. S., Ulep, T., Budiman, E., Safavinia, B., & Yoon, J. (2021, July). Emulsion nucleic acid amplification for bacterial identification via angle-dependent light scatter analysis. 31st Anniversary World Congress on Biosensors. Online: Elsevier.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Breshears, L. E., Mata Robles, S., Reynolds, K. A., & Yoon, J. (2021, Aug.). Flow rate profile based PFOA detection on paper-based microfluidics using competitive interactions with albumin and nitrocellulose. ACS Fall 2021 National Meeting & Exposition. Atlanta, GA: American Chemical Society (ACS).
  • Type: Book Chapters Status: Published Year Published: 2021 Citation: Laurel Dieckhaus, Tu San Park and Jeong-Yeol Yoon, "Smartphone Based Paper Microfluidic Immunoassay of Salmonella and E. coli," in Salmonella: Methods and Protocols, Third Edition, Editor: Heide Schatten, Humana Press: New York, 2021, pp.83-101.


Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Academic research; food safety, veterinary diagnostics, medical diagnostics, tissue engineering and emerging biotech industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?1 post-doctoral fellow, 9graduate students, and 10undergraduate students were mentored by the PI during the project period. How have the results been disseminated to communities of interest?1. Academic research: through disseminating our work in professional society's annual meetings (BMES),as well as seminars in universities and USDA-ARS. 2. Industry: through a series of discussion and seminars with industry. What do you plan to do during the next reporting period to accomplish the goals?1. Continue to publish top-quality, high-impact journal papers. Finalize and submit the Tissue Engineering Primer textbook to Springer. 2. Secure multidisciplinary research grants.

Impacts
What was accomplished under these goals? We have published 9journal papers in final form and 1 book.Our norovirus work has continuously received excellent media attention: NSF has highlighted our work ("Using a Smartphone to Detect Norovirus") in their NSF Research News, which has been picked up by University of Arizona Health Sciences and CEP Magazine from AIChE. Follow-up norovirus work has been submitted to Nature Protocols and received favorable review during the project period. (It was accepted in November 2020 and published in January 2021).It demonstratedextremely sensitive and rapid smartphone-based sensing of norovirus from environmental water samples.We havealso translatedour handheld biosensor technology into real-world practice, through collaborating with Tucson Water and Korea Institute of Ocean Science and Technology (KIOST), who have also provided research grants to our lab.I have secured three new grants from UArizona Health Sciences (smartphone detection of COVID-19), Tech Launch Arizona (smartphone microscope development), and internal NIFA project (THC detection from hemp). Tucson Water is succeeding our NSF WET Center grant, continuing on PFOA detection from wastewater.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Soo Chung, Christian M. Jennings and Jeong-Yeol Yoon, "Distance vs. Capillary Flow Dynamics-Based Detection Methods on Microfluidic Paper-Based Analytic Device," Chemistry - A European Journal, 2019, 25(57): 13070-13077.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Kattika Kaarj and Jeong-Yeol Yoon, "Methods of Delivering Mechanical Stimuli to Organ-on-a-chip," Micromachines, 2019, 10(10): 700.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Kaeun Lee, Hyunwoo Park, Sangho Baek, Seungjong Han, Dowan Kim, Soo Chung, Jeong-Yeol Yoon and Jongchul Seo, "Colorimetric Array Freshness Indicator and Digital Color Processing for Monitoring the Freshness of Packaged Chicken Breast," Food Packaging and Shelf Life, 2019, 22: 100408.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Tiffany-Heather Ulep, Ryan Zenhausern, Alana Gonzales, David S. Knoff, Paula A. Lengerke Diaz, Januario E. Castro and Jeong-Yeol Yoon, "Smartphone Based On-chip Fluorescence Imaging and Capillary Flow Velocity Measurement for Detecting ROR1+ Cancer Cells from Buffy Coat Blood Samples on Dual-layer Paper Microfluidic Chip," Biosensors and Bioelectronics, 2020, 153: 112042.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Matthew V. Bills, Andrew Loh, Katelyn Sosnowski, Brandon T. Nguyen, Sung Yong Ha, Un Hyuk Yim and Jeong-Yeol Yoon, "Handheld UV Fluorescence Spectrophotometer Device for the Classification and Analysis of Petroleum Oil Samples," Biosensors and Bioelectronics, 2020, 159: 112193.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kattika Kaarj, Jennifer Ngo, Christina Loera, Patarajarin Akarapipad, Soohee Cho and Jeong-Yeol Yoon, "Simple Paper-based Liver Cell Model for Drug Screening," BioChip Journal, 2020, 14(2): 218-229.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Matthew V. Bills and Jeong-Yeol Yoon, "Label-free Mie Scattering Identification of Tumor Tissue Using an Angular Photodiode Array," IEEE Sensors Letters, 2020, 4(7): 4500704.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kambiz Sadeghi, Jeong-Yeol Yoon and Jongchul Seo, "Chromogenic Polymers and Their Packaging Applications: A Review," Polymer Reviews, 2020, 60(3): 442-492.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kattika Kaarj, Marianne Madias, Patarajarin Akarapipad, Soohee Cho and Jeong-Yeol Yoon, "Paper-based In Vitro Tissue Chip for Delivering Programmed Mechanical Stimuli of Local Compression and Shear Flow," Journal of Biological Engineering, 2020, 14: 20.
  • Type: Books Status: Published Year Published: 2020 Citation: Jeong-Yeol Yoon, Editor & Author of First 3 Chapters, "Smartphone Based Medical Diagnostics," Elsevier: London/San Diego/Cambridge, 2020, ISBN: 978-0-12-817044-1.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Bills, M. V., & Yoon, J.-Y. (2019, October). Label-Free, Light Scatter Detection of Colorectal Carcinoma Using an Angular Photodiode Array. BMES Annual Meeting. Philadelphia, PA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Zenhausern, R., Ulep, T.-H., Gonzales, A., & Yoon, J.-Y. (2019, October). Dual Quantification of Chronic Lymphocytic Leukemia Using On- Chip Fluorescence Imaging-Based and Capillary Flow Dynamics-Based Paper Microfluidic Assays. BMES Annual Meeting. Philadelphia, PA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Day, A. S., Ulep, T.-H., Dieckhaus, L., Budiman, E., Safavinia, B., & Yoon, J.-Y. (2019, October). Emulsion Nucleic Acid Amplification Device for Septic Bacterium Identification via Mie Light Scatter. BMES Annual Meeting. Philadelphia, PA: BMES.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Academic research; food safety, veterinary diagnostics, medical diagnostics, tissue engineering and emerging biotech industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?6 graduate students and 15 undergraduate students were mentored by the PI during the project period. How have the results been disseminated to communities of interest?1. Academic research: through disseminating our work in professional society's annual meetings, including BMES, ASABE and ACS meetings, as well as seminars in universities and national laboratories. 2. Industry: through a series of discussion and seminars with industry. What do you plan to do during the next reporting period to accomplish the goals?1. Continue to publish top-quality, high-impact peer-reviewed publications. 2. Secure multidisciplinary research grants.

Impacts
What was accomplished under these goals? Summary of Work: There is a growing need to develop a handheld, smartphone-based biosensor that can detect the type and concentration of pathogens from myriads of food (fresh produce and meat) and water (waste and irrigation) samples, as well as urine, blood, and tissue samples from animal and human subjects. These biosensors must be designed and manufactured to be easy-to-use, all-in-one, and extremely sensitive (down to single cell level, single genomic copy level, or picogram protein level). During the period from October 1st, 2018 to September 30th, 2019, we have published 7 journal papers. In addition, there are four journal papers and one text book accepted in this period (not listed here - will be reported in the next year's report). Some of our work have received recognitions from professional societies, such as "Most Downloaded Journal Paper" from IEEE (listed #4 below) and "Highly Cited Journal Paper" from Royal Society of Chemistry (RSC) (Anal. Meth. 8: 6591-6601; published in late 2016 but the recognition was made in 2019). Most importantly, our work on single copy level detection of norovirus was selected one of only 17 papers (out of >12,000 papers) to be press-released by American Chemical Society (ACS) Fall 2019 National Meeting & Expo at San Diego Convention Center. There was also a press conference, broadcasted live on YouTube, and subsequently picked up by numerous news media, including NPR News from Washington Headquarter. We are also translating our handheld biosensor technology into real-world practice, through collaborating with Tucson Water and Korea Institute of Ocean Science and Technology (KIOST), who have also provided research grants to our lab.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Soo Chung, Lane E. Breshears and Jeong-Yeol Yoon, "Smartphone Near Infrared Monitoring of Plant Stress," Computers and Electronics in Agriculture, 2018, 154: 93-98.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Benjamin Alouidor, Robin E. Sweeney, Trinny Tat, Raymond K. Wong and Jeong-Yeol Yoon, "Microfluidic Point-of-care Ecarin Based Clotting and Chromogenic Assays for Monitoring Direct Thrombin Inhibitors," Journal of ExtraCorporeal Technology, 2019, 51: 29-37.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Katherine E. Klug, Christian M. Jennings, Nicholas Lytal, Lingling An and Jeong-Yeol Yoon, "Mie Scattering and Microparticle Based Characterization of Heavy Metal Ions and Classification by Statistical Inference Methods," Royal Society Open Science, 2019, 6: 190001.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Robin E. Sweeney, Vina Nguyen, Benjamin Alouidor, Elizabeth Budiman, Raymond K. Wong and Jeong-Yeol Yoon, "Flow Rate and Raspberry Pi-based Paper Microfluidic Blood Coagulation Assay Device," IEEE Sensors Journal, 2019, 19(13): 4743-4751.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Soo Chung, Lane E. Breshears, Sean Perea, Christina M. Morrison, Walter Q. Betancourt, Kelly A. Reynolds and Jeong-Yeol Yoon, "Smartphone-based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at Single Copy Level," ACS Omega, 2019, 4(6): 11180-11188.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Tiffany-Heather Ulep, Alexander S. Day, Katelyn Sosnowski, Alexa Shumaker and Jeong-Yeol Yoon, "Interfacial Effect-based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection," Scientific Reports, 2019, 9: 9629.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Matthew V. Bills, Brandon T. Nguyen and Jeong-Yeol Yoon, "Simplified White Blood Cell Differential: An Inexpensive, Smartphone- and Paper-Based Blood Cell Count," IEEE Sensors Journal, 2019, 19(18): 7822-7828.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ngo, J., Kaarj, K., & Yoon, J.-Y. (2018, October). Paper-based Liver Organ-on-a-chip for Herbal Drug Toxicity Screening. BMES Annual Meeting. Atlanta, GA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Zenhausern, R., Ulep, T.-H., Gonzales, A., & Yoon, J.-Y. (2018, October). Smartphone Based Vertical Flow Immunoassay of Circulating Tumor Cells. BMES Annual Meeting. Atlanta, GA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ulep, T.-H., Day, A., Sosnowski, K., Shumaker, A., & Yoon, J.-Y. (2018, October). Interfacial Effect-Based Real-Time Quantification of Droplet Isothermal Nucleic Acid Amplification. BMES Annual Meeting. Atlanta, GA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Kaarj, K., Madias, M., Cho, S., & Yoon, J.-Y. (2018, October). Flexible, Foldable and Reconfigurable Paper-based Angiogenesis Chip towards Developing Personalized Cancer Therapeutic Strategies. BMES Annual Meeting. Atlanta, GA: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Yoon, J.-Y. (2019, August). Smartphone-Based Device for Detecting Norovirus, the Cruise Ship Microbe. ACS Press Conference. San Diego, CA: ACS.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Chung, S., Breshears, L. E., Perea, S., Morrison, C. M., Betancourt, W. Q., Reynolds, K. A., & Yoon, J.-Y. (2019, August). Smartphone-Based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at Single Copy Level. ACS National Meeting & Expo. San Diego, CA: ACS.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Klug, K. E., Reynolds, K. A., & Yoon, J.-Y. (2019, August). Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring. ACS National Meeting & Expo. San Diego, CA: ACS.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Chung, S., Jennings, C., Perea, S., Yim, U. H., & Yoon, J.-Y. (2019, July). Classifying Origin of Crude Oil Using Raspberry Pi and Paper Microfluidic Chip. ASABE Annual International Meeting. Boston, MA: ASABE.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Academic research; food safety, veterinary diagnostics, medical diagnostics, tissue engineering and emerging biotech industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?9 graduate students and 17 undergraduate students were mentored by the PI during the project period. How have the results been disseminated to communities of interest?1. Academic research: through disseminating our work in professional society's annual meetings, including ASABE, ACS, IBE, KU-UA Joint International Symposium, BMES andKSBB meetings,as well asseminars in universities and national laboratories. 2. Industry: through a series of discussion and seminars with industry. What do you plan to do during the next reporting period to accomplish the goals?1. Continue to publish top-quality, high-impact peer-reviewed publications. 2. Secure multidisciplinary research grants.

Impacts
What was accomplished under these goals? The Mie scattering detection of particle immunoagglutination on microfluidic paper analytic devices (μPADs) have been successfully demonstrated using a smartphone. In this year, multiple steps of normalizations as well as mathematical interpolation algorithm have been developed to make this assay useable under varying ambient lighting conditions (Cho et al., 2017). Fluorescent particles were also utilized to make double detection of fluorescence and Mie scattering, to improve the assay credibility and reproducibility. This method was successfully demonstrated to detect cancer markers directly from undiluted serum and from 10% whole blood (Baynes and Yoon, 2018). In addition, challenges in fluorescence detection from paper platforms were also addressed (Ulep and Yoon, 2018). The same fluorescence detection on a paper platform was also demonstrated for real-time quantification of isothermal nucleic acid amplification (Kaarj et al., 2018). A wholly new concept was demonstrated utilizing the capillary flow dynamics as a transducing mechanism for detecting E. coli and Zika virus. The immunoagglutinated particles adsorb towards the liquid moving front in paper microfluidic channels, and such adsorption is linearly attenuated with immunoagglutination. This work was featured as an Inside Cover for Chemistry - A European Journal (Klug et al., 2018). The Mie scattering spectrum was collected from the simulated wound infection model to identify the species of bacterial infections, without using any antibodies, dyes, or reagents (Sweeney and Yoon, 2017).

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Kattika Kaarj, Patarajarin Akarapipad and Jeong-Yeol Yoon, "Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips," Scientific Reports, 2018, 8: 12438.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Tiffany-Heather Ulep and Jeong-Yeol Yoon, "Challenges in Paper-Based Fluorogenic Optical Sensing with Smartphones," Nano Convergence, 2018, 5: 14.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Katherine E. Klug, Kelly A. Reynolds and Jeong-Yeol Yoon, "A Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring," Chemistry - A European Journal, 2018, 24(23): 6025-6029. Hot Paper. Inside Cover. Highlighted in ChemistryViews Magazine.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Cayla Baynes and Jeong-Yeol Yoon, "�PAD Fluorescence Scattering Immunoagglutination Assay for Cancer Biomarkers from Blood and Serum," SLAS Technology, 2018, 23(1): 30-43.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Soohee Cho, Tu San Park, Kelly A. Reynolds and Jeong-Yeol Yoon, "Multi-Normalization and Interpolation Protocol to Improve Norovirus Immunoagglutination Assay from Paper Microfluidics with Smartphone Detection," SLAS Technology, 2017, 22(6): 609-615.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Robin E. Sweeney and Jeong-Yeol Yoon, "Angular Photodiode Array-Based Device to Detect Bacterial Pathogens in a Wound Model," IEEE Sensors Journal, 2017, 17(21): 6911-6917.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Chung, S., Breshears, L., Vedder, A., & Yoon, J.-Y. (2018, July). Smartphone Monitoring of Plant Water Stress. ASABE Annual International Meeting. Detriot, MI: ASABE.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Yoon, J.-Y. (2018, May). Biosensors for Healthcare-at-home and Personalized Medicine. 2nd KU-UA Joint International Symposium. Seoul, South Korea: Korea University.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Chung, S., Breshears, L. E., Morrison, C. M., Betancourt, W. Q., Reynolds, K. A., & Yoon, J.-Y. (2018, May). Single Particle Level Norovirus Detection Assay Using Smartphone-Based Fluorescence Microscope and a Microfluidic Paper Analytic Device. 2nd KU-UA Joint International Symposium. Seoul, South Korea: Korea University.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Bills, M. V., & Yoon, J.-Y. (2018, April). Cell Chromatography: Two-Layer, Paper-Based Microfluidic Purification and Quantification of Red Blood Cells, Granular, and Agranular White Blood Cells. Annual Meeting of IBE. Norfolk, VA: IBE.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Yoon, J.-Y. (2018, January). New Trends in Biosensor Research towards Healthcare-at-Home. 1st KU-UA Joint International Symposium. Tucson, AZ: University of Arizona.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ulep, T.-H., Day, A., & Yoon, J.-Y. (2018, January). Real-time Quantification of Droplet Isothermal Nucleic Acid Amplification through Measurements of Interfacial Effects. 1st KU-UA Joint International Symposium. Tucson, AZ: University of Arizona.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Chung, S., Breshears, L. E., Reynolds, K. A., & Yoon, J.-Y. (2018, January). Rapid and Reliable Norovirus Assay Using Smartphone-Based Fluorescence Microscope and a Microfluidic Paper Analytic Device. 1st KU-UA Joint International Symposium. Tucson, AZ: University of Arizona.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ulep, T.-H., Day, A., & Yoon, J.-Y. (2018, March). Real-Time Quantification of Droplet Isothermal Nucleic Acid Amplification through Measurements of Interfacial Effect. ACS National Meting. New Orleans, LA: ACS.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kaarj, K., Cho, S., & Yoon, J.-Y. (2017, October). Liver-on-a-paper model for drug screening. BMES 2017 Annual Meeting. Phoenix, AZ: BMES.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Yoon, J.-Y. (2017, October). [Keynote Speech] New Trends in Biosensor Research towards Healthcare-at-Home. KSBB Fall Meeting and International Symposium. Busan, South Korea: KSBB (Korean Society for Biotechnology and Bioengineering).


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Academic research; food safety, veterinary diagnostics, medical diagnostics, tissue engineering and emerging biotech industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?9 graduate students and 10 undergraduate students were mentored by the PI during the project period. How have the results been disseminated to communities of interest?1. Academic research: through disseminating our work in professional society's annual meetings, including IBE 2017, ASABE 2017, and CYTO 2017, as well as numerous seminars in universities and national laboratories. 2. Industry: through a series of discussion and seminars with industry. What do you plan to do during the next reporting period to accomplish the goals?1. Continue to publish top-quality, high-impact peer-reviewed publications. 2. Secure multidisciplinary research grants.

Impacts
What was accomplished under these goals? 1. Smartphone detection system has further been improved, with sophisticated image processing algorithms for eliminating noise. In addition, smartphone-based fluorescent microscope has also been developed and successfully visualized the immunoagglutination reactions on paper microfluidic devices. These improved systems have successfully been demonstrated for detecting pathogens (E. coli, norovirus and Zika virus) as well as environmental toxicants (bisphenol A and heavy metal ions). 2. Smartphone- and paper-based organ-on-a-chip system has newly been developed and used as a mimic for human kidney and liver. The effects of environmental toxicants have successfully been demonstrated using this new platform. 3. An angular photodiode array system was designed and fabricated to instantly analyze the bacterial infection on porcine skin (as a model for human skin infection), without using any bioreceptors (antibodies, enzymes, or DNA) or reagents.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Robin E. Sweeney, Elizabeth Budiman and Jeong-Yeol Yoon, "Mie Scatter Spectra-Based Device for Instant, Contact-Free, and Specific Diagnosis of Bacterial Skin Infection," Scientific Reports, 2017, 7: 4801.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Soohee Cho and Jeong-Yeol Yoon, "Organ-on-a-Chip for Assessing Environmental Toxicants," Current Opinion in Biotechnology, 2017, 45: 34-42.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Katherine E. McCracken, Trinny Tat, Veronica Paz and Jeong-Yeol Yoon, "Smartphone-Based Fluorescence Detection of Bisphenol A from Water Samples," RSC Advances, 2017, 7: 9237-9243.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Tu San Park, Soohee Cho, Tigran G. Nahapetian and Jeong-Yeol Yoon, "Smartphone Detection of UV LED Enhanced Particle Immunoassay on Paper Microfluidics," SLAS Technology, 2017, 22(1): 7-12.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ariana M. Nicolini, Katherine E. McCracken and Jeong-Yeol Yoon, "Future Developments in Biosensors for Field-Ready Zika Virus Diagnostics," Journal of Biological Engineering, 2017, 11: 7.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Jeong-Yeol Yoon, "Towards the 10-Year Milestone of Journal of Biological Engineering," Journal of Biological Engineering, 2017, 11: 3.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Katherine E. McCracken, Trinny Tat, Veronica Paz, Kelly A. Reynolds and Jeong-Yeol Yoon, "Immunoagglutinated Particle Rheology Sensing on a Microfluidic Paper-Based Analytical Device for Pathogen Detection," ASABE Annual International Meeting, Spokane, WA, July 16-19, 2017. p. 1701190.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Soo Chung, Lane E. Breshears, Soohee Cho, Kelly A. Reynolds and Jeong-Yeol Yoon, "Rapid and Reliable Norovirus Assay at pg/mL Level Using Smartphone-Based Fluorescence Microscope and a Microfluidic Paper Analytic Device," ASABE Annual International Meeting, Spokane, WA, July 16-19, 2017. p. 1701234.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: David W. Galbraith, Jeong-Yeol Yoon, M. Alejandra Mandel, Ariana M. Nicolini and Tyler D. Toth, "In situ optical detection of pathogens using emulsion loop-mediated isothermal amplification," CYTO2017, Congress of the International Society for Advancement of Cytometry, Boston, MA, June 10-14, 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jeong-Yeol Yoon, "Immunoagglutinated Particle Rheology Detection from Paper Microfluidic Analytic Device for E. coli and Zika Virus Assays," Annual Meeting of IBE, Salt Lake City, UT, March 30 - April 1, 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jeong-Yeol Yoon, "Organ-on-a-Chip for Assessing Environmental Toxicants," Annual Meeting of IBE, Salt Lake City, UT, March 30 - April 1, 2017.