Progress 09/01/15 to 08/31/20
Outputs
Target Audience:Veterinary and graduate students in the College of Veterinary Medicine at Tuskegee University were the primary targetaudience. However, other students in the university that attend the Annual Biomedical Research Symposium as well as theAnnual Veterinary Symposium were also exposed to the project activities. Research papers were presented at the local research symposia including: George Washington Carver Lecture Series and the Annual Biomedical Research syposium. Three papers were also presented at the Conference of Research Workersin Animal Disease (CRWAD) in Chicago, Illinois,December 2018 and 2019. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Ms. Kiara Cousin completed her PhD dissertation work with reagents and diagnostic kits provided from grant funds. She submitted the dissertation titled: The use of iron oxide nanoparticle-based receptor conjugates for capture and rapid detection of Salmonella serovars. Eight veterinary students participated in the Summer Research Internships and received stipends. Names of these veterinarystudents: Ms. Shantavia Hayes, Ms. Onyx Goins, Ms. Allena Edmonds, Mr. John Delgado, Ms. Imani Williams, Mr. SageShaddok, Ms. Jazmine Pritchett and Ms. Khadijah Martin. They received training on the following topics: 1. Introduction to LabSafety and Biosafety, 2. Using Library Resources to Support Research, 3. Bioethics in Science and Research, and 4.Laboratory Animal Handling and Ethics of Animal Use. Graduate Students received training in the use of Scanning ElectronMicroscopy and the new instrument (NanoBrook 90PlusPALS) purchased for use in research related to nanotechnologyapplications.The eight students also participated in International Internship opportunity in the Summer in veterinary colleges at Suez CanalUniversity and Sadat City University in Egypt How have the results been disseminated to communities of interest?Graduate student Ms. Rawah Faraj presented a paper at the 99th Conference of Research workers in Animal Disease(CRWAD), Chicago, IL, December 1-4, 2018. The title of the paper was: LipL32 and /or LipL21 of Leptospira conjugated withenterotoxin B of E. coli induce high titers of antibodies. She also presented another paper: Rawah Faraj*, Woubit Abdela,Temesgen Samuel, Gopal Reddy. Novel Approach for Production of Recombinant E. coli Enterotoxin B and Leptospira LipL32for the Development of Vaccine against Leptospirosis. PepTalk, The Protein Science Week, January 8-12, 2018, Hilton SanDiego Bayfront, San Diego, CA.The students that participated in the Summer research program made oral or poster presentations on their research at the19th Annual Biomedical Research symposium at Tuskegee University, September 20-21, 2018. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Hands-on experiences in basic laboratory diagnostic procedures were provided to 2nd year veterinary students in theVirology/ Immunolaboratory course and to 3rd & 4th year students in the Microbiology Clinical Rotation classes. Interpretationof the results pertaining to use of diagnostic tests using Agglutination, ELISA, Agar-gel Immunodiffusion test, and otherserological tests.8 Veterinary students were provided stipends to participate in on-going research projects of a few faculty members in theCollege of Veterinary Medicine during summer of 2019. Four of the students presented posters during the 2018 AnnualBiomedical Research Symposium, September 21-23, 2019.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Sayma Afroj, Khaled Aldahami, Gopal Reddy, Jean Guard, Abiodun Adesiyun, Temesgen Samuel, Woubit Abdela. Simultaneous detection of multiple Salmonella serovars from milk and chicken meat by real-time PCR using unique genomic target regions. Journal of Food Protection, 2017, Vol. 80, No. 11, pp. 1944-1957
Hafiz, S., Srivastava, K.K., Newton, J.C., Samaha, H., Hassan, A., and Reddy, G. Efficacy of Curcumin as an Immunostimulatory Dietary Supplement for Channel Catfish. American Journal of Animal and Veterinary Sciences. 10.3844/ajavsp.2017.1.7., Volume 12, Issue 1
Afroj, S., Aldahami, K., Reddy, G., Guard, J., Adesiyun, A., Samuel, T., Abdela, W. Simultaneous detection of multiple Salmonella serovars from milk and chicken meat by real-time PCR using unique genomic target regions. Journal of Food Protection, 2017, Vol. 80, No. 11, pp. 1944-1957
Byomi A, Zidan S, Diab M, Reddy G, Abiodun Adesiyun, and Abdela Woubit (2017). Characterization of Diarrheagenic Escherichia coli Serotypes Isolated from Poultry and Humans. SOJ Vet Sci 3(1): 1-8.
Mohamed A, Reddy GP, Samuel T, Mansour M, Woubit Abdela (2016) Clostridium difficile (Cd) in Shelter Dogs: Rationale for Screening of Pets Slated for Adoption. SOJ Vet Sci 2(1): 1-6.
Rawah Faraj*, Ehsan Abdalla, Gopal Reddy, Temesgen Samuel, Woubit Abdela*. Chimera of Heat labile Enterotoxin B chain conjugate with LipL32 and or LipL21 proteins induces strong reactive antibodies in experimental mice "IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) 4. 3 (2018): 58-68.
Possible ramifications of climate variability on HPAI-H5N1 outbreak occurrence: Case study from the Menoufia, Egypt. Yumna Elsobky, Gamal El Afandi, Ehsan Abdalla, Ahmed Byomi, Gopal Reddy, 16 Dec 2020 PLOS ONE
https://doi.org/10.1371/journal.pone.0244360.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2019
Citation:
1. Surveillance for Tick-borne Pathogens in East Alabama. Master's in Veterinary Science Thesis, May 2019. Author:Hamid Mahama
2. Isolation, Ribotyping and Toxin Characterization ofClostridiodes difficile in Companion Animals and Food. PhD Thesis, July 2019. Abdelrahman Mohamed.
3. Effect of Hemp seed meal on the performance and immunity of growing meat goats. PhD dissertation. December 2020. Frank Abrahamsen.
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Progress 09/01/18 to 08/31/19
Outputs
Target Audience:Veterinary and graduate students in the College of Veterinary Medicine at Tuskegee University were the primary target audience. However, other students in the university that attend the Annual Biomedical Research Symposium as well as the Annual Veterinary Symposium were also exposed to the project activities. Research papers were presented at the local research symposia including: George Washington Carver Lecture Series and the AnnualBiomedicalResearch syposium. Two papers were also presented at the Conference of Research Workersin Animal Disease (CRWAD) in Chicago, Illinois, December 2-4, 2018. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Eight veterinary students participated in the Summer Research Internships and received stipends. Names of these veterinary students: Ms. Shantavia Hayes, Ms. Onyx Goins, Ms. Allena Edmonds, Mr. John Delgado, Ms. Imani Williams, Mr. Sage Shaddok, Ms. Jazmine Pritchett and Ms. Khadijah Martin. They received training on the following topics: 1. Introduction to Lab Safety and Biosafety, 2. Using Library Resources to Support Research, 3. Bioethics in Science and Research, and 4. Laboratory Animal Handling and Ethics of Animal Use. Graduate Students received training in the use of Scanning Electron Microscopy and the new instrument (NanoBrook 90PlusPALS) purchased for use in research related to nanotechnology applications. The eight students also participated in International Internship opportunity in the Summer in veterinary colleges at Suez Canal University and Sadat City University in Egypt. How have the results been disseminated to communities of interest?Graduate student Ms. Rawah Faraj presented a paper at the 99th Conference of Research workers in Animal Disease (CRWAD), Chicago, IL, December 1-4, 2018. The title of the paper was: LipL32 and /or LipL21 of Leptospira conjugated with enterotoxin B of E. coli induce high titers of antibodies. She also presented another paper: Rawah Faraj*, Woubit Abdela, Temesgen Samuel, Gopal Reddy. Novel Approach for Production of Recombinant E. coli Enterotoxin B and Leptospira LipL32 for the Development of Vaccine against Leptospirosis. PepTalk, The Protein Science Week, January 8-12, 2018, Hilton San Diego Bayfront, San Diego, CA. The students that participated in the Summer research program made oral or poster presentations on their research at the 19th Annual Biomedical Research symposium at Tuskegee University, September 20-21, 2018. What do you plan to do during the next reporting period to accomplish the goals? The unencumbered funds of approximately $60,000 will be used mainly to support of two graduate students Mr. Frank Abrahamsen and Ms. Kiara Cousin expected to graduate in May 2020 and stipends for DVM students working on the project objectives. Research work of the graduate student Ms. Kiara Cousin, was delayed because the previous Mentor and PI for this grant left Tuskegee University Her research is progressing now and will graduate in May 2020. The other PhD student Mr. Abrahamsen started his research in August 2018. We will purchase additional research supplies related to on-going work of these two students with the grant funds. Mrs. Zainab Alwan PhD student is receiving partial support and will complete her PhD work by May 2020. Mr. Hamed Mahama who received his master's degree in Veterinary Science in May 2019 is expected to present a paper at the 2020 American Society of Microbiology meetings in May 2020 and will be provided travel funds. His research focused on detection of flaviviruses in ticks collected on animal patients in veterinary teaching hospital. To continue the research work done by Mr. Mahama, we will engage three veterinary students to collect ticks and mosquitos, isolate RNA and test for the presence of flaviviruses. We will further enhance syllabi for the Veterinary Virology & Immunology courses for veterinary students. The syllabi was updated and the courses were retitled as: Infection & Immunity II and Infection & Immunity III.
Impacts
What was accomplished under these goals?
Hands-on experiences in basic laboratory diagnostic procedures were provided to 2nd year veterinary students in the Virology/ Immunolaboratory course and to 3rd & 4th year students in the Microbiology Clinical Rotation classes. Interpretation of the results pertaining to use of diagnostic tests using Agglutination, ELISA, Agar-gel Immunodiffusion test, and other serological tests. 8 Veterinary students were provided stipends to participate in on-going research projects of a few faculty members in the College of Veterinary Medicine during summer of 2018. Four of the students presented posters during the 2018 Annual Biomedical Research Symposium, September 21-23, 2018.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
1. Rawah Faraj*, Ehsan Abdalla, Gopal Reddy, Temesgen Samuel, Woubit Abdela*. Chimera of Heat labile Enterotoxin B chain conjugate with LipL32 and or LipL21 proteins induces strong reactive antibodies in experimental mice "IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) 4. 3 (2018): 58-68.
2. Gines R.B, Collier E. W, Abdalla A.M, and Yehualaeshet T. 2018. Influence of Metabolite Extraction Methods on 1H-NMR-Based Metabolomics Profiling of Enteropathogenic Yersinia. Methods Protoc. Nov. 2018, 1(4), 45; https://doi.org/10.3390/mps1040045.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
1. Rawah Faraj, Ehsan Abdella, Temesgen Samuel, Gopal Reddy and Woubit Abdela. LipL32 and /or LipL21 of Leptospira conjugated with enterotoxin B of E. coli induce high titers of antibodies. The 99th Annual Conference of Research Workers in Animal Diseases (CRWAD), Dec 1-4, 2018, Chicago Marriott, Downtown Magnificent Mile, Chicago, Illinois
2. Rawah Faraj, Woubit Abdela, Temesgen Samuel, Gopal Reddy. Novel Approach for Production of Recombinant E. coli Enterotoxin B and Leptospira LipL32 for the Development of Vaccine against Leptospirosis. PepTalk, The Protein Science Week, January 8-12, 2018, Hilton San Diego Bayfront, San Diego, CA
3. Kirstie McTear, Rawah Faraj, Gopal Reddy, Woubit Abdela, Temesgen Samuel. Comparing Different Detection Methods of Leptospira. 19th Annual One Health Symposium, 7th annual Phi Zeta Research Day. September 20-21, 2018, Tuskegee University, AL
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2019
Citation:
1. Surveillance for Tick-borne Pathogens in East Alabama. Master's in Veterinary Science Thesis, May 2019. Author: Hamid Mahama
2. Isolation, Ribotyping and Toxin Characterization of
Clostridiodes difficile in Companion Animals and Food. PhD Thesis, July 2019. Abdelrahman Mohamed
|
Progress 09/01/17 to 08/31/18
Outputs
Target Audience:Veterinary and graduate students in the College of Veterinary Medicine at Tuskegee University. Research papers werepresented at the local research symposia including: George Washington Carver Lecture Series and the Annual BiomedicalResearch syposium at Tuskegee University. Two papers were also presented, one at the Conference of Research Workersin Animal Disease (CRWAD) in Chicago, Illinois, December 3-6, 2017 and the other at the 2017 American Society ofMicrobiiology meetings in San Diego, CA Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? 6 veterinary students participated in the Summer Research Internships and recerived stipends. They also traveled to Egypt for International Internship at veterinary clinics attached to Faculty of Veterinary Medicine at Suez canal University and Alexandria University. Mr. Hamed Mahama, Graduate student was provided travel support to get trained in the laboratory of Dr. Solomon Odemuyiwa (previous PI for this grant), Associate Professor at University of Missouri, College of Veterinary Medicine,Columbia, MO. Ms. Montessa Mitchell was provided travel support to attend the 2017 Annual Conference of the Research Worker's in AnimalDisease, December 3-6, 2017, Chicago, IL. How have the results been disseminated to communities of interest?Ms. Montessa Mitchell, Graduate student presented a paper at the national conference - 2017 Annual Conference of Research Worker's in Animal Disease, December 3-6, 2017 at Chicago, IL. More than 500 scientists attend this annual conference. She and Ms. Nadia Al-Altoum presented research papers at the Annual One Health Symposia at Tuskegee University. This conference is attended by about 20 scientists and graduate students from regional institutions including some1890 institutions. What do you plan to do during the next reporting period to accomplish the goals?Syllabi for the DVM courses of "Virology" and "Virology & Immunology Lab" will be further revised and updated with multimedia presentations with focus on problem-based approach. We will enhance syllabus to cover some aspects ofnanobiotechnology in the courses: 1) Advanced pathogenic Microbiology and 2) Immunology. We will engage four veterinary students in the summer of 2018 to collect more mosquitoes, isolate RNA and test for the presence of flaviviruses. Mr. Hamed Mahama is expected to present a paper at the 2018 American Society of Microbiology meetings.
Impacts
What was accomplished under these goals?
Laboratory hands-on experiences have been provided to students taking virology /Immunology laboratory course using various ELISA, AGID kits and other serological tests. 4 veterinary students were provided stipends to work in the laboratories of PI and Co-PI during the summer for a period of 10-weeks. These students presented posters during the 2017 Annual Biomedical Research symposium at Tuskegee University during the month of September 2017.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Sayma Afroj, Khaled Aldahami, Gopal Reddy, Jean Guard, Abiodun Adesiyun, Temesgen Samuel, Woubit Abdela. Simultaneous detection of multiple Salmonella serovars from milk and chicken meat by real-time PCR using unique genomic target regions. Journal of Food Protection, 2017, Vol. 80, No. 11, pp. 1944-1957
Hafiz, S., Srivastava, K.K., Newton, J.C., Samaha, H., Hassan, A., and Reddy, G. Efficacy of Curcumin as an Immunostimulatory Dietary Supplement for Channel Catfish. American Journal of Animal and Veterinary Sciences. 10.3844/ajavsp.2017.1.7., Volume 12, Issue 1
Afroj, S., Aldahami, K., Reddy, G., Guard, J., Adesiyun, A., Samuel, T., Abdela, W. Simultaneous detection of multiple Salmonella serovars from milk and chicken meat by real-time PCR using unique genomic target regions. Journal of Food Protection, 2017, Vol. 80, No. 11, pp. 1944-1957
Byomi A, Zidan S, Diab M, Reddy G, Abiodun Adesiyun, and Abdela Woubit (2017). Characterization of Diarrheagenic Escherichia coli Serotypes Isolated from Poultry and Humans. SOJ Vet Sci 3(1): 1-8.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Mitchell, M., N. Pastrana-Negron, A. Mohammed, P. G. Reddy and S.O. Odemuyiwa. 2017. Surveillance and Characterization of Non-Primate Hepacivirus C in East Alabama. Paper presented at the 2017 Annual Conference of Research Workers in Animal Disease, December 3-6, 2017,Chicago, IL.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2017
Citation:
Surveillance and Characterization of Non-Primate Hepacivirus C in East Alabama by Graduate Student, Ms. Montessa Mitchell.
|
Progress 09/01/16 to 08/31/17
Outputs
Target Audience:Veterinary and graduate students in the College of Veterinary Medicine at Tuskegee University. Research papers werepresented at the local research symposia including: George Washington Carver Lecture Series and the Annual BiomedicalResearch syposium at Tuskegee University. Two papers were also presented, one at the Conference of Research Workersin Animal Disease (CRWAD) in Chicago, Illinois, December 3-6, 2017 and the other at the 2017 American Society ofMicrobiiology meetings in San Diego, CA. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Mr. Hamed Mahama, Graduate student was provided travel support to get trained in the laboratory of Dr. SolomonOdemuyiwa (previous PI for this grant), Associate Professor at University of Missouri, College of Veterinary Medicine,Columbia, MO.Ms. Montessa Mitchell was provided travel support to attend the 2017 Annual Conference of the Research Worker's in AnimalDisease, December 3-6, 2017, Chicago, IL How have the results been disseminated to communities of interest?Ms. Montessa Mitchell, Graduate student presented a paper at the national conference - 2017 Annual Conference ofResearch Worker's in Animal Disease, December 3-6, 2017 at Chicago, IL. More than 500 scientists attend this annualconference. She and Ms. Nadia Al-Altoum presented research papers at the Annual One Health Symposia at TuskegeeUniversity. This conference is attended by about 20 scientists and graduate students from regional institutions including some1890 institutions. What do you plan to do during the next reporting period to accomplish the goals?Syllabi for the DVM courses of "Virology" and "Virology & Immunology Lab" will be further revised and updated withmultimedia presentations with focus on problem-based approach. We will enhance syllabus to cover some aspects ofnanobiotechnology in the courses: 1) Advanced pathogenic Microbiology and 2) Immunology. We will engage four veterinarystudents in the summer of 2017 to collect more mosquitoes, isolate RNA and test for the presence of flaviviruses. Mr. Hamed Mahama is expected to present a paper at the 2017 American Society of Microbiology meetings and also submit his Master'sthesis to graduate in May 2017.
Impacts
What was accomplished under these goals?
Syllabi for the Virology & Immunology laboratory course for the veterinary & graduate students was updated to provide morehands-on experience. Reagents & ELISA kits were used in teaching basic laboratory skills. 3 veterinary students were provided stipends to work in the laboratories of PI and Co-PI during the summer for a period of 10-weeks. Mosquito traps were set up to collect mosquitoes in the local area. These mosquitoes will be tested for the presenceof alphaviruses and flaviviruses using specific-primers in real-time PCR assays.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Mitchell, M., N. Pastrana-Negron, A. Mohammed, P. G. Reddy and S.O. Odemuyiwa. 2017. M. Mitchell, N. Pastrana-Negron, A. Mohammed, P. G. Reddy and S.O. Surveillance and Characterization of Non-Primate Hepacivirus C in EastAlabama. Paper presented at the 2017 Annual Conference of Research Workers in Animal Disease, December 3-6, 2017,Chicago, IL.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2017
Citation:
Surveillance and Characterization of Non-Primate Hepacivirus C in East Alabama by Graduate Student, Ms. MontessaMitchell.
|
Progress 09/01/15 to 08/31/16
Outputs
Target Audience:Veterinary and graduate students in the College of Veterinary Medicine at Tuskegee University. Research paperswere presented at the local research symposia including: George Washington Carver Lecture Series and the Annual Biomedical Research syposium at Tuskegee University. Two papers were also presented, one at the Conference of Research Workers in Animal Disease (CRWAD) in Chicago, Illinois, December 3-6, 2017 and the other at the 2017 American Society of Microbiiology meetings in San Diego, CA. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Mr. Hamed Mahama, Graduate student was provided travel support to get trained in the laboratory of Dr. Solomon Odemuyiwa (previous PI for this grant), Associate Professor at University of Missouri, College of Veterinary Medicine, Columbia, MO. Ms. Montessa Mitchell was provided travel support to attend the 2017 Annual Conference of the Research Worker's in Animal Disease, December 3-6, 2017, Chicago, IL. How have the results been disseminated to communities of interest?Ms. Montessa Mitchell, Graduate student presented a paper at the national conference - 2017 Annual Conference of Research Worker's in Animal Disease, December 3-6, 2017 at Chicago, IL. More than 500 scientists attend this annual conference. She and Ms. Nadia Al-Altoum presented research papers at the Annual One HealthSymposia at Tuskegee University. This conference is attended by about 20 scientists and graduate students from regional institutionsincluding some 1890 institutions. What do you plan to do during the next reporting period to accomplish the goals?Syllabi for the DVM courses of "Virology" and "Virology & Immunology Lab"will be furtherrevised and updated with multimedia presentations with focus on problem-based approach. Wewill enhance syllabus to cover some aspects of nanobiotechnology in the courses: 1) Advanced pathogenic Microbiology and 2) Immunology. We will engage four veterinary students in the summer of 2019 to collect more mosquitoes, isolate RNA and test for the presence of flaviviruses. Mr. Hamed mahama is expected to present a paper at the 2019 American Society of Microbiology meetings and also submit his Master's thesis to graduate in May 2019.
Impacts
What was accomplished under these goals?
Syllabi for the Virology & Immunology laboratory course for the veterinary & graduate students was updated to provide more hands-on experience. Reagents & ELISA kits were used in teaching basic laboratory skills. 3 veterinary students were provided stipends to work in the laboratories of PI and Co-PI during the summer for a period of 10-weeks. Mosquito traps were set up to collect mosquitoes in the local area. These mosquitoes will be tested for the presence of alphaviruses and flaviviruses using specific-primers in real-time PCR assays.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Nadia Al-Atoum, Corwin Spears, Jr., and Toufic Nashar. Phage display for identification of peptides that bind Zika-virus surface exposed protein E. 19th Annual One Health Symposium, September 20-21, 2018, Tuskegee University, Tuskegee, AL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Mitchell, M., N. Pastrana-Negron, A. Mohammed, P. G. Reddy and S.O. Odemuyiwa. 2017. M. Mitchell, N. Pastrana-Negron, A. Mohammed, P. G. Reddy and S.O. Surveillance and Characterization of Non-Primate Hepacivirus C in East Alabama. Paper presented at the 2017 Annual Conference of Research Workers in Animal Disease, December 3-6, 2017, Chicago, IL.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2017
Citation:
Surveillance and Characterization of Non-Primate Hepacivirus C in East Alabama by Graduate Student, Ms. Montessa Mitchell.
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