Source: AGRICULTURAL RESEARCH SERVICE submitted to
CLONING, CHARACTERIZATION, AND VALIDATION OF PUP1/P EFFICIENCY IN MAIZE
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Accession No.
0427220
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Apr 1, 2015
Project End Date
Nov 30, 2015
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
ITHACA,NY 14853
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
10%
Research Effort Categories
Basic
70%
Applied
10%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1330110102015%
2021510103040%
2031520104015%
2041540102010%
1331550103020%
Knowledge Area
204 - Plant Product Quality and Utility (Preharvest); 133 - Pollution Prevention and Mitigation; 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants; 202 - Plant Genetic Resources;

Subject Of Investigation
1510 - Corn; 1550 - Barley; 1540 - Hard red winter wheat; 0110 - Soil; 1520 - Grain sorghum;

Field Of Science
1040 - Molecular biology; 1030 - Cellular biology; 1020 - Physiology;
Goals / Objectives
A multidisciplinary team involving USDA, Embrapa, JIRCAS, IRRI and Moi University will work on the identification and characterization of genes associated with maize P efficiency (tolerance to low P). The objectives for this research project include: 1. Pup-1 candidate gene identification in maize 2. QTL/gene mapping for P use efficiency in maize 3. Inheritance studies on maize root architecture under high and low P 4. Validation of maize Pup-1 candidate genes and if necessary, novel P efficiency QTL (if maize Pup1 homologues are not functional in P efficiency)
Project Methods
A multidisciplinary team involving USDA, Embrapa, JIRCAS, IRRI and Moi University will work on the identification and characterization of genes associated with maize P efficiency (tolerance to low P). Bioinformatics will be used to identify homologues of the rice Pup-1 (P uptake efficiency) gene in maize and a set of markers for these genes will be developed. An Embrapa inbred line panel that was developed for breeding for P efficiency be phenotyped in the field for this trait measured as grain yield under contrasting P conditions and in the greenhouse/lab for root architecture traits; also the Buckler association panel will be phenotyped for P efficiency and root architecture traits in the green house/lab at USDA-ARS. Finally, a maize RIL population from the cross of a highly P efficient tropical maize line (L3) with a P inefficient line (L22) will be phenotyped to identify QTLs for P acquisition, internal P efficiency and root architecture traits. Also, candidate genes for p efficiency will be mapped on the same RIL population, in order to verify the co-segregation of Pup-1 homologs with QTLs for different P-efficiency traits. Finally, association analysis using Embrapa¿s elite inbred lines panel and the Buckler maize association panel will be carried out to validate candidate genes.