Progress 06/01/07 to 05/31/12
Outputs
Progress Report Objectives (from AD-416): The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416): Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. The project is a collaborative effort with the University of Arkansas scientists working in the University of Arkansas Center for Food Safety on the Fayetteville campus. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members, and the expression of specific microbial genes within communities. In the past year we have continued to make progress in several research projects related to this cooperative agreement. A study initiated last year focused on the response of lactic acid bacteria to Maillard reaction product derivatives.
Impacts
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Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416) Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. The project is a collaborative effort with the University of Arkansas scientists working in the Center for Food Safety and Microbiology on the Fayetteville campus. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members, and the expression of specific microbial genes within communities. In the past year we have made progress in several research projects related to this cooperative agreement. In the first project we have developed a multiplex quantitative PCR method that allows us to simultaneously detect and quantify Salmonella spp., Campylobacter spp., and Escherichia coli O157:H7. This work has now been published in a peer reviewed journal in 2011. A second study initiated this year focuses on the response of lactic acid bacteria to Maillard reaction product derivatives, and a peer reviewed paper that examines transcriptomic responses of Salmonella Typhimurium to these Maillard reaction products has now been accepted for publication. ADODR is in continual contact with the cooperators via meetings, email, and phone conversations to evaluate progress.
Impacts
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416) Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. The project is a collaborative effort with the University of Arkansas scientists working in the Center for Food Safety and Microbiology on the Fayetteville campus. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. In the past year we have made progress in several research projects related to this cooperative agreement. In the first project we have developed a multiplex quantitative PCR method that allows us to simultaneously detect and quantify Salmonella spp., Campylobacter spp., and Escherichia coli O157:H7. Some of this work was presented at the 2010 American Society for Microbiology meetings. In a second study we continue research examining the effect of cold shock on the long-term survival of bifidobacteria. A third study initiated this year focuses on comprehensive genetic profiling of gastrointestinal tract microflora in birds after the addition of different biological feed additives. ADODR is in continual contact with the cooperators via meetings, email, and phone conversations to evaluate progress.
Impacts
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416) Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. Significant Activities that Support Special Target Populations The project is a collaborative effort with the University of Arkansas scientists working in the Center for Food Safety and Microbiology on the Fayetteville campus. ADODR is in continual contact with the cooperators via meetings, email, and phone conversations to evaluate progress. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. In the past year we have continued 2 research projects related to this cooperative agreement. In the first project we determined the inhibitory effect of the probiotic bacteria Bifidobacterium adolescentis ATCC 15703 on selected human pathogens, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, and Staphylococcus aureus, and presented some of this work at the 2009 American Society for Microbiology meetings. We are now exploring emulsifying agents that may help with delivery of some of these compounds to enhance their inhibitory effect on foodborne pathogens without influencing probiotic cultures. In a second study we continued research to examine whether certain carbohydrates support growth of bifidoabacteria after undergoing chemical reactions during food processing. A third study initiated this year focuses on genetic profiling of GI tract microflora after drastic dietary changes in the gut.
Impacts
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Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416) Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. Significant Activities that Support Special Target Populations The project is a collaborative effort with the University of Arkansas scientists working in the Center for Food Safety and Microbiology on the Fayetteville campus. ADODR is in continual contact with the cooperators via meetings, email, and phone conversations to evaluate progress. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members, and the expression of specific microbial genes within communities. In the past year we have initiated 2 research projects related to this cooperative agreement. In the first project we determined the inhibitory effect of the probiotic bacteria Bifidobacterium adolescentis ATCC 15703 on selected human pathogens, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, and Staphylococcus aureus, and preliminary results indicate that inhibitory effect on these respective pathogens may depend on growth state of the probiotic culture. In a second study we are initiating research to examine why only certain carbohydrates support growth of bifidoabacteria, which leads to our next step towards focusing on genetic screening for isolating prebiotics that enhance bifidobacterial colonization in the gut.
Impacts
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research is to develop and use molecular tools to study the mechanisms and functionality of probiotic cultures for reducing foodborne pathogen colonization in poultry. Approach (from AD-416) Recent success using probiotic application to limit colonization of foodborne pathogens in the gastrointestinal tracts of food animals ensures continued commercialization and widespread use of these cultures. However, if further optimization of the effectiveness of defined cultures is to be achieved, methods to assess expression of key metabolic processes occurring during the establishment of the probiotic culture as well as its subsequent ability to limit food borne pathogen colonization are needed. Molecular tools will be used to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities. Methods will include the utilization of both DNA and RNA targeted probes, gene amplification protocols and mRNA analysis. Significant Activities that Support Special Target Populations This report serves to document research conducted under a specific cooperative agreement between the University of Arkansas and ARS. Additional details of the research can be found in the report for the in- house associated project 6226-32000-009-00D, Investigating the impact of stress on foodborne pathogen colonization in turkeys. The project is a collaborative effort with the University of Arkansas scientists working in the Center for Food Safety and Microbiology on the Fayetteville campus. ADODR is in continual contact with the cooperators via meetings, email and phone conversations to evaluate progress. Molecular tools are being developed to study individual members of microbial consortia, fluxes in community diversity, spatial distribution of consortia members and the expression of specific microbial genes within communities.
Impacts
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