Progress 01/15/07 to 01/14/11
Outputs
Progress Report Objectives (from AD-416) Develop and demonstrate procedures for stable transformation of chloroplast genome of plants without creating serious disruptions to photoysnthesis and to normal chloroplast metabolism. Determine inheritance patterns of genes selected for insertion and what limitations, if any, are imposed by plastid transformation rather than nuclear transformation. Utilize new procedures to develop plants capable of synthesizing high-value products, such as pharmaceuticals, or capable of enhanced productivity. Approach (from AD-416) Genetic engineering of plants has tremendous potential to create crops with new metabolic capabilities, like the ability to accumulate pharamecuticals or other high-value specialty materials. In addition to metabolic engineering itself, however, there are some obstacles to be overcome before these technologies can be commercialized. One is the potential for spread of transgenes from the engineered plants to conventional fields of the same crop, largely by dispersal of pollen. An attractive approach to the problem of gene dispersal is to genetically engineer the chloroplast male gametophyte (pollen). Work conducted over the life of this agreement has significantly advanced chloroplast transformation for use in biotechnology applications for the production of novel products in plants. The research progress resulting from this agreement was (1) the determination of the complete sequence of the cassava chloroplast genome; (2) demonstration that chloroplast transformation of tobacco is effective in the phytoremediation of mercury and organomercurials from contaminated soils; (3) demonstration of effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts; (4) chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars. Research activities were monitored by the ADODR by semi-annual written reports.
Impacts
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Publications
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) Develop and demonstrate procedures for stable transformation of chloroplast genome of plants without creating serious disruptions to photoysnthesis and to normal chloroplast metabolism. Determine inheritance patterns of genes selected for insertion and what limitations, if any, are imposed by plastid transformation rather than nuclear transformation. Utilize new procedures to develop plants capable of synthesizing high-value products, such as pharmaceuticals, or capable of enhanced productivity. Approach (from AD-416) Genetic engineering of plants has tremendous potential to create crops with new metabolic capabilities, like the ability to accumulate pharamecuticals or other high-value specialty materials. In addition to metabolic engineering itself, however, there are some obstacles to be overcome before these technologies can be commercialized. One is the potential for spread of transgenes from the engineered plants to conventional fields of the same crop, largely by dispersal of pollen. An attractive approach to the problem of gene dispersal is to genetically engineer the chloroplast male gametophyte (pollen). This year�s progress included: (1) Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars; (2) the role of heterologous chloroplast sequence elements in transgene integration and expression; (3) genetic engineering to enhance mercury phytoremediation. The work advances chloroplast transformation for use in several biotechnology applications. Research activities were monitored by the ADODR by semi-annual written reports.
Impacts
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Publications
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) Develop and demonstrate procedures for stable transformation of chloroplast genome of plants without creating serious disruptions to photoysnthesis and to normal chloroplast metabolism. Determine inheritance patterns of genes selected for insertion and what limitations, if any, are imposed by plastid transformation rather than nuclear transformation. Utilize new procedures to develop plants capable of synthesizing high-value products, such as pharmaceuticals, or capable of enhanced productivity. Approach (from AD-416) Genetic engineering of plants has tremendous potential to create crops with new metabolic capabilities, like the ability to accumulate pharamecuticals or other high-value specialty materials. In addition to metabolic engineering itself, however, there are some obstacles to be overcome before these technologies can be commercialized. One is the potential for spread of transgenes from the engineered plants to conventional fields of the same crop, largely by dispersal of pollen. An attractive approach to the problem of gene dispersal is to genetically engineer the chloroplast male gametophyte (pollen). Significant Activities that Support Special Target Populations This year�s progress included: (1) the determination of the complete nucleotide sequence of the cassava chloroplast genome; (2) demonstration that chloroplast transformation of tobacco is effective in the pytoremediation of mercury and organomercurials; (3) demonstration of effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts. The work advances chloroplast transformation for use in several biotechnology applications. Research activities were monitored by the ADODR by semi-annual written reports.
Impacts
(N/A)
Publications
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Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) Develop and demonstrate procedures for stable transformation of chloroplast genome of plants without creating serious disruptions to photoysnthesis and to normal chloroplast metabolism. Determine inheritance patterns of genes selected for insertion and what limitations, if any, are imposed by plastid transformation rather than nuclear transformation. Utilize new procedures to develop plants capable of synthesizing high-value products, such as pharmaceuticals, or capable of enhanced productivity. Approach (from AD-416) Genetic engineering of plants has tremendous potential to create crops with new metabolic capabilities, like the ability to accumulate pharamecuticals or other high-value specialty materials. In addition to metabolic engineering itself, however, there are some obstacles to be overcome before these technologies can be commercialized. One is the potential for spread of transgenes from the engineered plants to conventional fields of the same crop, largely by dispersal of pollen. An attractive approach to the problem of gene dispersal is to genetically engineer the chloroplast male gametophyte (pollen). Significant Activities that Support Special Target Populations This year�s progress included: (1) the determination of the complete nucleotide sequence of the cassava chloroplast genome; (2) demonstration that chloroplast transformation of tobacco is effective in the pytoremediation of mercury and organomercurials; (3) demonstration of effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts. The work advances chloroplast transformation for use in several biotechnology applications. Research activities were monitored by the ADODR by semi-annual written reports.
Impacts
(N/A)
Publications
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