Progress 01/01/07 to 10/31/08
Outputs
Progress Report Objectives (from AD-416) Develop scanning force microscopy (SFM) and Surface Enhanced Raman Spectroscopy (SERS) as tools for the detection of viral pathogens through antigen/antibody binding. Approach (from AD-416) Step 1. a. Construct dual analyte capture substrates using calicivirus and parvovirus as a two antigen model system. b. Construct two sets of immunogold labels. 1. The set for the Raman labels will require two different Raman scatterers, using the position of the vibrational mode for identification and intensity for quantification. 2. Two different sizes of immunogold labels will be employed, with the size of the particle used for identification and the number bound to the capture surface for quantification. Step 2. a. Test assay platforms with spiked serum samples. b. Repeat studies for different pathogens, including BVDV. Step 3. a. Expand efforts to incorporate various approaches to sample concentration directly in line with the on chip readout process. b. Expand the prototype tests to include detection of multiple viruses using viral models that represent high consequence pathogens (HCP) and viruses that are regarded as type viruses for their family or genus. The first type of test will be for specific viruses that are either HCP or endemic viruses that may be confused with HCP based on clinical presentation. The second type of test will be a differential test for viral families or genera. Step 4. a. Prepare written final report, including a critical discussion of the future opportunities for these assay methodologies as "point of use" techniques for meeting NADC needs. b. Discuss opportunities with NADC for future collaborations. Significant Activities that Support Special Target Populations The objective of this agreement was to develop atomic force microscopy (AFM) and surface enhanced Raman spectroscopy (SERS) as tools for the detection of viral pathogens through antigen/antibody binding. Past work with these technologies demonstrated their ability to detect viral pathogens present at low levels in experimental samples. Work conducted thus far has pushed the sensitivity of the SERS technology as well as decreased the detection time through modifications made to sample application and geometry of the SERS particles used as the detection label. Results of this past year�s activities resulted in a prototype test, using SERS as the readout technology, that allows detection of two viruses simultaneously with no loss in sensitivity or specificity. Oversight of the project was maintained by meeting with the cooperator monthly. National Program 103 Animal Health: Component 5: Countermeasures to Prevent and Control Reproductive and Neonatal Diseases.
Impacts
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Develop scanning force microscopy (SFM) and Surface Enhanced Raman Spectroscopy (SERS) as tools for the detection of viral pathogens through antigen/antibody binding. Approach (from AD-416) Step 1. a. Construct dual analyte capture substrates using calicivirus and parvovirus as a two antigen model system. b. Construct two sets of immunogold labels 1. The set for the Raman labels will require two different Raman scatterers, using the position of the vibrational mode for identification and intensity for quantification. 2. Two different sizes of immunogold labels will be employed, with the size of the particle used for identification and the number bound to the capture surface for quantification. Step 2. a. Test assay platforms with spiked serum samples. b. Repeat studies for different pathogens, including BVDV Step 3. a. Expand efforts to incorporate various approaches to sample concentration directly in line with the on chip readout process. b. Expand the prototype tests to include detection of multiple viruses using viral models that represent high consequence pathogens (HCP) and viruses that are regarded as type viruses for their family or genus. The first type of test will be for specific viruses that are either HCP or endemic viruses that may be confused with HCP based on clinical presentation. The second type of test will be a differential test for viral families or genera. Step 4. a. Prepare written final report, including a critical discussion of the future opportunities for these assay methodologies as "point of use" techniques for meeting NADC needs. b. Discuss opportunities with NADC for future collaborations. Significant Activities that Support Special Target Populations This report serves to document accomplishments under a Specific Cooperative Agreement between ARS and Arizona State University. Additional details of research can be found in the parent project 3625- 32000-070-00X, Tools for Differentiation of High Consequence Pathogens and Endemic Viruses. The objective of this SCA was to develop scanning force microscopy (ASM) and surface enhanced Raman spectroscopy (SERS) as tools for the detection of viral pathogens through antigen/antibody binding. Past work with these technologies demonstrated their ability to detect viral pathogens present at low levels in experimental samples. Work conducted thus far has pushed the sensitivity of the SERS technology as well as decreased the detection time through modifications made to sample application and geometry of the SERS particles used as the detection label. Oversight of the project was maintained by meeting with the cooperator monthly.
Impacts
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