Progress 01/02/03 to 01/01/08
Outputs
Progress Report Objectives (from AD-416) This study is designed to determine if the water extract of cinnamon, which is high in polyphenols, improves antioxidant status of rats fed a high fructose diet to induce decreased insulin sensitivity. Approach (from AD-416) Weanling Wistar rats, males, will be fed a fructose rich diet which is known to produce insulin resistance and early signs of the metabolic syndrome. This rat model mimics early signs of type 2 diabetes. Group I will receive ad libitum the fructose rich diet; Group II the fructose rich diet containing 0.2 g of water extract of cinnamon (Cinnulin)/100g of diet and Group III the fructose rich diet containing 2 g of Cinnulin per g diet (Cinnulin will be provided by CRADA partner, Integrity Nutraceuticals). Blood sampling and tissue (kidney, muscle, pancreas and liver) removal will take place at the beginning of week 9. Samples related to antioxidant variables will be analyzed in laboratory of collaborator in France and samples regarding insulin function sent to ARS laboratory for analyses. Significant Activities that Support Special Target Populations This Agreement was set up to determine if a water extract of cinnamon (CinnulinPF), which is high in polyphenols, improves antioxidant status of rats fed a high fructose diet to decrease insulin sensitivity. Thirty Wistar rats, 6 weeks old, were divided into three groups and fed a high fructose diet to reduce insulin sensitivity. One group of 10 rats continued on the same diet, and groups of two or three were given the high fructose diet plus 2 or 20 g of the CinnulinPF per kg of diet. Rats receiving the Cinnulin PF displayed decreased blood glucose, insulin, and triglyceride levels and improved antioxidant status. Animals receiving the higher level of the Cinnulin PF displayed greater improvements. Follow-up human studies demonstrated that the aqueous CinnulinPF also had beneficial effects on glucose, insulin, and antioxidant status, of people with the metabolic syndrome. In conclusion, addition of an aqueous extract of cinnamon to experimental animals and humans with diet-induced reduced insulin sensitivity, led to beneficial effects on oxidative stress markers, lipemia, and glycemia. This is the final report; this project was monitored by emails and telephone communications.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) This study is designed to determine if the water extract of cinnamon, which is high in polyphenols, improves antioxidant status of rats fed a high fructose diet to induce decreased insulin sensitivity. Approach (from AD-416) Weanling Wistar rats, males, will be fed a fructose rich diet which is known to produce insulin resistance and early signs of the metabolic syndrome. This rat model mimics early signs of type 2 diabetes. Group I will receive ad libitum the fructose rich diet; Group II the fructose rich diet containing 0.2 g of water extract of cinnamon (Cinnulin)/100g of diet and Group III the fructose rich diet containing 2 g of Cinnulin per g diet (Cinnulin will be provided by CRADA partner, Integrity Nutraceuticals). Blood sampling and tissue (kidney, muscle, pancreas and liver) removal will take place at the beginning of week 9. Samples related to antioxidant variables will be analyzed in laboratory of collaborator in France and samples regarding insulin function sent to ARS laboratory for analyses. Significant Activities that Support Special Target Populations This report documents research conducted under a trust agreement between ARS and Integrity Neutraceuticals International, Sarasota, FL. Additional details of research can be found in the report for the associated in-house project 1235-51520-037-00D, "Chromium and Polyphenols from Cinnamon in the Prevention and Alleviation of Glucose Intolerance". The causes and control of type 2 diabetes mellitus are not clear, but there is strong evidence that dietary factors are involved in its regulation and prevention. One group of male weanling Wistar rats was fed a high-fructose diet that has been shown to induce insulin resistance, a second group the same diet plus 0.1 g grams of dried water soluble extract of cinnamon (Cinnulin PF, to be furnished by CRADA partner), and the third group the high fructose diet plus 2 g of Cinnulin PF. Animals have been fed the diet and samples are being analyzed. Conference calls were held on a bi-monthly basis and two yearly on-site visits were made by the cooperator.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report documents research conducted under a Cooperative Research and Development Agreement (CRADA) with Integrity Neutraceuticals International, Sarasota, FL. Additional details of the research can be found in the report for the parent project 1235-51520-037-00D, entitled "Chromium and polyphenols from cinnamon in the prevention and alleviation of glucose intolerance." The causes and control of type 2 diabetes mellitus are not clear but there is strong evidence that dietary factors are involved in its regulation and prevention. Extracts from cinnamon enhance the activity of insulin. The objective of this study is to isolate and characterize insulin enhancing complexes from cinnamon that may be involved in the alleviation or possible prevention and control of glucose intolerance and diabetes. Water-soluble polyphenolic polymers have been isolated and characterized from cinnamon that increase insulin-dependent in vitro glucose
metabolism roughly 20-fold and also function as antioxidants. Studies are in progress in cell culture and rats to determine the mode of action of these complexes. A collaborative human study was completed and will be presented at annual meeting of the American College of Nutrition. A second human study is being initiated regarding the effects of a water soluble extract on obese people with insulin resistance.
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