Progress 03/10/04 to 03/09/09
Outputs
Progress Report Objectives (from AD-416) To provide a framework for cooperation to promote the efficient and safe diagnosis of emerging diseases and/or contaminants that could affect animal and human health. The ARS, Arthropod-Borne Animal Diseases Research Lab (ABADRL) collaborates with several entities on research projects aiming to improve the diagnosis of, and protections against, infectious agents carried by insects. The Wyoming Dept. of Agriculture (WDA) is the state agency responsible for microbiological and chemical qualitative and quantitative analysis to protect health and the quality- of-life of agricultural and urban communities. The WDA Analytical Service Lab (ASL) is charged to conduct thorough study and analysis to ensure clean water, a safe food supply and proper pesticide and fertilizer use. Approach (from AD-416) (1) Detect and positively identify contaminants and microbiological agents in feed, fertilizer, forage, soils, livestock, pesticides and surface and waste water sources that could affect arthropod viability or have an effect on livestock health in regard to arthropod/insect vectored diseases; (2) Develop and implement testing and diagnostic procedures for current and emerging contaminant and microbiological threats; (3) Create a secure bank of contaminant and microbiological samples from Wyoming; and (4) Investigate the link of arthropod and livestock interactions in areas where microbiological and chemical processes are at work that promote or inhibit the spread of disease. Significant Activities that Support Special Target Populations Over the lifetime of the agreement, surveillance of the presence of West Nile virus and bluetongue virus in trapped insect vectors in Laramie, Wyoming was conducted to determine the prevalence of disease and the need for vector control. No mosquito or Culicoides vectors were tested for arboviruses between the beginning of FY 09 and the end of the agreement. The ADODR maintained contacts via email.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) To provide a framework for cooperation to promote the efficient and safe diagnosis of emerging diseases and/or contaminants that could affect animal and human health. The ARS, Arthropod-Borne Animal Diseases Research Lab (ABADRL) collaborates with several entities on research projects aiming to improve the diagnosis of, and protections against, infectious agents carried by insects. The Wyoming Dept. of Agriculture (WDA) is the state agency responsible for microbiological and chemical qualitative and quantitative analysis to protect health and the quality- of-life of agricultural and urban communities. The WDA Analytical Service Lab (ASL) is charged to conduct thorough study and analysis to ensure clean water, a safe food supply and proper pesticide and fertilizer use. Approach (from AD-416) (1) Detect and positively identify contaminants and microbiological agents in feed, fertilizer, forage, soils, livestock, pesticides and surface and waste water sources that could affect arthropod viability or have an effect on livestock health in regard to arthropod/insect vectored diseases; (2) Develop and implement testing and diagnostic procedures for current and emerging contaminant and microbiological threats; (3) Create a secure bank of contaminant and microbiological samples from Wyoming; and (4) Investigate the link of arthropod and livestock interactions in areas where microbiological and chemical processes are at work that promote or inhibit the spread of disease. Significant Activities that Support Special Target Populations This report documents research conducted under a Non-Funded Cooperative Agreement between ARS and the Wyoming Dept. of Agriculture. Additional details of research can be found in the report for the in-house associated project 5410-32000-014-00D, Vector Competence and Protection of U.S. Livestock and Wildlife from Arthropod-borne Diseases. Surveillance of the presence of West Nile Virus and bluetongue virus in trapped insect vectors was conducted to determine the prevalence of disease and the need for vector control. Discussions and a report were filed on the need for BSL3 containment facilities in Wyoming. Planning and design of two facilities (one in Cheyenne and one in Laramie) are proceeding. ADODR monitoring activities to evaluate research progress were made via telephone, meetings, email, etc.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under Non-Funded Cooperative Agreement between ARS and the Wyoming Department of Agriculture, Cheyenne, WY ("Detection of Arthropod-Borne Diseases and Protection of Livestock"). Additional details of research can be found in the report for the parent project, #5410-32000-014-00D, Vector Competence and Protection of U.S. Livestock and Wildlife from Arthropod-Borne Diseases. Mosquitoes were trapped in the Powder River Basin of Wyoming and those who had visible bloodmeals were used to determine what the host source of the bloodmeal was to assist with identification of various sources of West Nile virus in the environment. Bloodmeal hosts were identified through cytochrome b PCR analyses. Assays were largely of three mosquito species. Culex tarsalis (72 individuals) had the widest host range including: deer, cow, pronghorn antelope, human, rabbit, sparrow, moose, pheasant, duck, hawk,
oriole, snipe, vole, owl, sage grouse. Aedes vexans (93 individuals) had the next widest host range including: deer, pheasant, moose, cow, pronghorn antelope, human, vole, and sparrow. Aedes dorsalis (65 individuals) had the smallest host range including: cow, pronghorn antelope, deer, human, rabbit, moose.
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under Non-Funded Cooperative Agreement #58-5410-4-111N between ARS and the Wyoming Department of Agriculture, Cheyenne, WY. Additional details of research can be found in the report for the parent project 5410-32000-014-00D. ABADRL scientists developed a high throughput IR dye diagnostic method for detection of bluetongue virus (BTV) in Culicoides. Sensitivity of the method is 1 pfu from an extraction of 10 pfus. All 24 BTV serotypes can be detected. Epizootic hemorrhagic disease virus serotypes are apparently not detected by this method. ABADRL scientists trapped vectors around Wyoming and determined populations of bluetongue and West Nile vector species, percent of trapped vectors carrying bluetongue and West Nile virus, and bloodmeal hosts for those insects with bloodmeals. Mosquito blood meal analyses revealed deer, cattle, antelope, jack rabbit, sparrow, skunk, vole, duck, sage grouse, human,
oriole, and owl as hosts. About 0.06-0.07% of mosquito vectors (Culex tarsalis, C. pipiens, Aedes vexans, Psorophora sp. , Culiseta inornata, Ochlerotatus dorsalis, O. melanimon, O. campestris, O. trivittatus) were positive for West Nile virus; no Culicoides were found to be positive for West Nile virus (19,445 were tested). Of ca. 22, 000 Culicoides trapped 9,000 have been tested for the presence of BTV. None of the tested samples were positive for BTV.
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Progress 10/01/03 to 09/30/04
Outputs
4. What were the most significant accomplishments this past year? This report serves to document research conducted under an agreement between ARS and the Wyoming Department of Agriculture. Additional details of research can be found in the report for the parent project 5410-32000- 010-00D.
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