Source: LOUISIANA STATE UNIVERSITY submitted to
NATIONAL ANIMAL HEALTH LABORATORY - NAHLN:LA
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
EXTENDED
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
0230082
Grant No.
2012-37620-19789
Project No.
LAV-2408-12N
Proposal No.
2012-03360
Multistate No.
(N/A)
Program Code
AA-G
Project Start Date
Aug 15, 2012
Project End Date
Aug 14, 2015
Grant Year
2015
Project Director
Roy, A.
Recipient Organization
LOUISIANA STATE UNIVERSITY
202 HIMES HALL
BATON ROUGE,LA 70803-0100
Performing Department
Louisiana Animal Disease Diagnostic Laboratory
Non Technical Summary
The Louisiana Animal Disease Diagnsotic Laboratory is the State Diagnostic Laboratory in Louisiana. The laboratory performs routine diagnostic tests for endemic animal disease as well as targeted surveillance and in responds to situations by laboratory testing for foreign animal diseases that could have devasting effect on the animal industries of the state and nation. The state laboratory also participates in the development of new assay and methods for the most advanced type to testing for detection of diseases that effect the animal food supply to this nation and export potential of the nation. The testing performed pertains to the beef, pork and poutlry industry of the United States.
Animal Health Component
90%
Research Effort Categories
Basic
(N/A)
Applied
90%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113999104070%
3113999110110%
3113999110010%
3113999116010%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3999 - Animal research, general;

Field Of Science
1101 - Virology; 1160 - Pathology; 1100 - Bacteriology; 1040 - Molecular biology;
Goals / Objectives
The founding principles of the National Animal Health Laboratory Network (NAHLN) include implementation of a quality management system, quality standards, proficiency testing, trained personnel, and secure communication with an alert reporting system. The Louisiana Animal Disease (LADDL) continues to strive to accomplish the goals of the NAHLN. Results of the highest quality and reliability are only achieved by laboratories that maintain the principles involved in quality assurance systems. Development and implementation of a quality assurance system within a laboratory is process and resource driven. LADDL continues to make progress in its quality assurance system with training, proficiency testing, and document control. LADDL continues to improve the processing, handling, and biocontainment of Foreign Animal Disease (FAD) samples using standard operating procedures (SOPs) and training. LADDL's Proposed Objectives for Improvement of the Laboratory's Quality System includes: Increase involvement of the Laboratory's staff in quality assurance activities. Initiate a review of the Laboratory's Quality Management System (QMS) and report deficiencies to the Laboratory's Administrators in management review. Continue professional development and technical training. Train staff to recognize and detect foreign and emerging animal diseases through workshops and courses when available. Calibration and monitoring of environmental conditions that effect quality to testing. Continue to participate in external proficiency testing programs from external sources such as the Veterinary Laboratory Association, National Veterinary Service and NAHLN. Sample Handling, Processing, and Biocontainment Capabilities Involved in Diagnostic Testing of NAHLN Samples includes: Management of NAHLN standard operating procedures. Procedures for receiving, handling, storage, and final disposition of NAHLN samples for diagnostic testing of endemic animal diseases, targeted surveillance and foreign animal diseases.
Project Methods
LADDL's Proposed Objectives for Improvement of the Laboratory's Quality System include: Increase involvement of the Laboratory's staff in quality assurance activities such as auditing and documenting nonconformities and corrective actions. Investigate "online" quality system training for the Laboratory's staff. Initiate a review of the Laboratory's QMS and report deficiencies to the Laboratory's Administrators in management review. Continue professional development and technical training with workshops and symposiums. LADDL's QAC and other member of the laboratory will continue to attend quality assurance training sessions and NAHLN information technology conferences that are sponsored by the AAVLD or other organizations to help advance LADDL's Quality Assurance Program and Reporting and Messaging within the NAHLN. Train staff to recognize and detect foreign and emerging animal diseases through workshops and courses when available. Calibration of scales, balances using the ANSI\ASTM Class 1 electronic balance calibration weight sets that are NIST/NVLAP certified. Calibration of pipettes using an external company (Bio-Tek Services) and internal calibration using Pipette Tracker (Labtronics, Inc.) which is a pipette and volumetric calibration software. Validation of real time PCR instruments will be accomplished using the Driftcon Temperature measurement system (BIOplastics/CYCLERtest Inc.) and Dye Calibration Kit for ABI 7500 instrument(Applied Biosystems) Continue to monitor and record all environmental conditions (temperature and humidity) that affect testing within the laboratories. NIST traceable devices are used to monitor and record environmental parameters (temperature and humidity) in refrigerators, freezers, incubators, and laboratories so as to assure the quality of testing has not been affected by unacceptable environmental conditions. Continue to participate in external proficiency testing programs from external sources such as the Veterinary Laboratory Association, National Veterinary Service and NAHLN. A laboratory document control system that will mandate an assignment of a unique document control number to each document assigned "controlled" status, requires the QAC to maintain a current and comprehensive master document list of all of LADDL's controlled documents, allows only the QAC to issue, control and archive controlled document originals and distribute their true copies to respective laboratory sections, and requires the QAC to audit laboratory sections for the presence of uncontrolled documents and/or non-current copies of controlled documents. The laboratory will address sample Handling, processing, and biocontainment capabilities involved in diagnostic testing of NAHLN samples by management of NAHLN Standard Operating Procedures,procedure for receiving, handling, storage, and final disposition of NAHLN Samples for diagnostic testing of endemic animal diseases and targeted surveillance,andforeign animal diseasesprocedure Outputs will be evaluated by internal audits by the quality assurance coordinator.Nonconformances will be documneted with corrective and prevention actions taken by the laboratory

Progress 08/15/14 to 08/14/15

Outputs
Target Audience:Target audiences for this project include the animal industries in the state of Louisiana and the United States. The primary animal industries affected are the cattle, and poultry industry. The State Animal Health Officals and the Federal Animal Health Officials are also target audience for this project. Consumers of the animal industry products are also affect by the work performed in this project and are a target audience. The efforts of this work are explained in formal classroom instruction to veterinary students. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Members of the staff have been trained by the use of proficiency test the ability to detect foreign animal disease using a laboratory approach. Training in auditing, document control and nonconformance recognition have also been accomplished. Members of the staff have been trained in outbreak response ,of a FAD to include diagnostic capacity capability ,and resource planning. How have the results been disseminated to communities of interest?Verbal and written communication with the State Animal Health Officials and Federal ADD's. What do you plan to do during the next reporting period to accomplish the goals?Continue training by attending national meetings, workshops and conference calls. Continue to improve and advance the laboratory's Quality Assurance Program. Continue to participate in proficiency testing.

Impacts
What was accomplished under these goals? Accomplishments include the continued advancement of the laboratory's quality assurance system and training of staff. The laboratory participated in quality assurance training, safety training, exercise and drill training for foreign animal diseases and attended scientific session during meetings and workshops. The quality assurance training afforded members of the laboratory staff a better understanding of internal audits, nonconformances, and corrective and preventive action requirements. Members of the laboratory participate in foreign animal disease scenario exercise training.

Publications


    Progress 08/15/13 to 08/14/14

    Outputs
    Target Audience: Target audiences for this project include the animalindustries in the state of Louisiana and the United States. The primary animal industriesaffected arethe cattle, and poultry industry. The State Animal Health Officals and the Federal Animal Health Officials are also target audience for this project. Consumers of the animal industry products are also affect by the work performed in this project and are a target audience. The efforts of this work are explained in formal classroom instruction to veterinary students. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Members of the staff have been trained by the use of proficiency testi the ability to detect foreign animal disease using a laboratory approach. Training in auditing,document control and nonconformance recognition have alsobeen accomplished. Members of the staff have been trained in outbreak respons,of a FAD to include diagnsotic capacity capabililty,and resource planning. How have the results been disseminated to communities of interest? Verbal and written communication with the State Animal Health Officials and Federal ADD's. What do you plan to do during the next reporting period to accomplish the goals? Continue training by attending national meetings, woirkshops and conference calls. Continue to improve and advance the laboratory's Quality Assurance Program. Continue to participate in proficiency testing.

    Impacts
    What was accomplished under these goals? Accomplishments include the continued advancement of the laboratory's quality assurance system and training of staff. The laboratory participated inquallity assurance training, safety training, exercise and drilltraining for foreignanimal diseasesand attended scientifc session during meetings and workshops.The quality assurance training affordedmembers of the laboratory staff a better understanding of internal audits, nonconformances,and corrective and preventive action requirements. Members of the laboratory participate in foreign animal diseasescenario exericse training and outbreak response guideline development.

    Publications


      Progress 08/15/12 to 08/14/13

      Outputs
      Target Audience: The Human and animal population of the U.S. as well as the animal industry Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Continue doing the same thing to accomplish the goals.

      Impacts
      What was accomplished under these goals? LADDL is one of the 12 laboratories in the U. S. that make up the National Animal Health Laboratory work. The main goal of the laboratories is the detection and reporting of Foreign Animal Diseases in the US. This is what was accomplished.

      Publications