Progress 09/01/13 to 08/31/14
Outputs
Target Audience:The target audience was mainly undergraduate and graduate students in the disciplines of agriculture, environmental and life sciences at North Carolina A&T State University. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Over the life of this award (2011-2015) there have been numerous training and professional development activities and events associated with this project. For example, there have been hands-on trainingsand experiential learning activities (30); course lectures (9); seminars (10); conference presentations/participations (35); and professional development events (3) which have enhanced proteomics knowledge and skills of faculty and students at the undergraduate and graduate level. How have the results been disseminated to communities of interest?Project participants have sponsored workshops, hands-on training events/activities, proteomics lab tours to visitors (e.g., prospective students, community organizations including K-12 students and youth organizations). Community engagement activities (e.g., lab tours, conferences, recruitment tours, seminars, educational forums and field days) provided platforms to share training opportunities and learning outcomes. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Resources from NIFA were leveraged to address the lack of proteomics instrumentation and resources at N.C. A&T State University. Three major outcomes have resulted from this project: 1)a proteomics laboratory was established at North Carolina A&T State University in 2012; 2) hands-on training and lecture modules were developed; 3) undergraduate and graduate students, faculty, and researchers participated in proteomics-focused educational and/or research activities as part of this project. There were a number of proteomics project related lectures, seminars/presentations, training activities totaling 91 events over a four year period (2011-2015). This project impacted a total of 1,371 people -- consisting of undergraduate and graduate students, professors, scientists/researchers and community members - by providing access to proteomics expertise, instrumentation and knowledge.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Ranabhat R, McClendon CJ, Souza e Silva AA, Pettiford SG, Conklin DR, Waterman JT. 2015. Swine production systems cause differential expression of superoxide dismutase in porcine tracheobronchial tissues. American Journal of Respiratory and Critical Care Medicine 191:A1718.
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Progress 09/01/12 to 08/31/13
Outputs
Target Audience: The primary target audience was undergraduate and graduate students in the disciplines of agricultural, environmental and life sciences at North Carolina A&T State University. This year there was an effort to reach middle and high school students also. We were also interested in targeting teaching and research faculty, and staff so that the knowledge gained will be transferred to students for years to come. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Hands-on research training opportunities were provided for student research trainees (15 total) -- doctoral (3), master of science (4), and undergraduate students (8). Twenty-five (25) middle and high school students participated in hands-on activities in the proteomics laboratory during summer 2013. How have the results been disseminated to communities of interest? During Year 2, there were eight (8) conference presentations and nine (9) lectures/seminar. Conference Presentations (8 presentations, 162 participants impacted) Minor R, Luster-Teasley S, Alford V, Locklear J. “Engaging Middle School Girls in STEM with Case Studies and Hands-on Lab Experiences: A College Professor’s Experience.” 14th Annual Conference on Case Study Teaching in Science. September 20, 2013. UB-SUNY Buffalo, NY (15 participants) McClendon C, Pettiford SG, Conklin D, Laremore TN, Oh S-H, Waterman JT. 2013. Proteomics analysis of the impact of swine production style on airway morphology. American Thoracic Society International Conference. Philadelphia, PA. May 17-22, 2013. (15 participants) McClendon C, Oh S-H, Waterman JT. 2013. Analysis of the impact of production style on porcine airway morphology and proteome dynamics. ARD 17th Biennial Research Symposium. Jacksonville, FL. April 6-10, 2013. 3rd Place Graduate Student Oral Presentation Award. (40 participants) Minor T, Waterman JT, Idassi J, Smith D. Moringa Oleifera Tea Abrogates Inflammation. Association of Research Directors (ARD) Conference. Jacksonville, FL. April 6-10, 2013. (40 participants) McKnight M, Smith D, Hurley H, Waterman JT, Idassi J, Minor RC. 2013. Moringa Tea Decreases c-jun n-Terminal Kinase Activation in a Model of Acute Airway Inflammation. Minorities in Agriculture Natural Resources and Related Sciences (MANNRS). Sacramento, CA. March 21-23, 2013. (30 participants) Pettiford SG, Conklin CJ, McClendon CJ, Waterman JT. 2013. Histological evaluation of porcine airways from animals reared indoors and outdoors. Fayetteville State University Student Research Conference. Fayetteville, NC. March 12-13, 2013. (7 participants) McDaniel TC, McPherson CN, Gerald CL, Xu, K, Waterman JT. 2013. Quantitative Analysis of Lipopolysaccharides in Agricultural Dusts. FSU Student Research Conference. Fayetteville, NC. March 12-13, 2013. (5 participants) Minor RC, Waterman JT, Allen J, Smith D, Hurley S. “Moringa Oleifera Tea Abrogates Neutrophil Influx but not TNF-alpha Production” Society for Leukocyte Biology Annual Meeting- October 2012. Maui, Hi. (10 participants) Lectures and Seminars (9 activities, 188 participants) Lectures in 3 courses (ANSC 637, LASC 660, BIOL 755) Seminars (6) Waterman, J.T. 2013. “Activities of Toxicants-Part I.” ANSC 637 - Environmental Toxicology Course. Lecture with a component on the application of proteomics technologies to the science of toxicology, toxicoproteomics. Instructor: Jenora Waterman, Ph.D. Fall 2013 (16 participants) Muganda P. 2013. “Proteomics Workflows.” Department of Biology, North Carolina A&T State University. (BIOL 755) Host: Perpetua Muganda, Ph.D. April 2013 (8 participants) Waterman JT. 2013. The DIRT on agricultural dust. Agricultural Research Seminar Series. North Carolina A&T State University. March 21, 2013. (20 participants) Minor RC. 2013. “Diet and Lung- Inflammation: A Tale of Two Supplements” Biology Department Seminar, Department of Biology, North Carolina A&T State University. (15 participants) Minor RC. 2013. “Diet and Immune Modulation: An Investigation of Two Supplements, Beta glucan and Moringa Oleifera.” Departmental Seminar, East Carolina University. (20 participants) Minor RC. 2013. LASC 660-Special Techniques in Specimen Preparation, Immunological Techniques, Electron Microscopy, Radiology or Histotechnology Course. Western Blot Technique. Proteomics laboratory was used for gel imaging. Spring 2013. (14 participants) Minor RC. 2012. “Diet and Lung- Inflammation: A Tale of Two Supplements” presented at A Day in the Life of a Cell- Journal Club. Winston Salem State University. (35 participants) Minor RC. 2012. “Modulating Immunity Through Diet” Agricultural Research Seminar, North Carolina A&T State University. (30 participants) Minor RC. 2012. “Diet and Lung- Inflammation: A Tale of Two Supplements” Departmental Seminar, Biomedical Biotechnology Research Institute (BBRI), North Carolina Central University. (30 participants) Outreach (2 activities, 25 participants) Waterman, J.T. 2013. “Proteomics is Cool…It Really Is.” Visit by Middle and High School Group – Calvary Christian Center Youth Council. Host: Jenora Waterman, PhD, July 10, 2013. (12 participants) Minor RC, Luster-Teasley S. 2013. “Girls in Science” Middle School Summer Camp. Proteomics laboratory was used for imaging gels to visualize PCR products. North Carolina A&T State University. June 2013. (13 participants) What do you plan to do during the next reporting period to accomplish the goals? In the final year of the PrIME award, we will continue to teach and train students throught course and laboratory activities. We plan to share results through conference presentations and publications.
Impacts
What was accomplished under these goals?
Proteomics lectures and training materials were utilized in three courses: ANSC 637 - Environmental Toxicology BIOL 755 - Systems Biology LASC 660 - Special Techniques in Specimen Preparation, Immunological Techniques, Electron Microscopy, Radiology or Histotechnology
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
McClendon C, Pettiford S, Conklin D, Laremore TN, Oh S-H, Waterman JT. 2013. Proteomics analysis of the impact of swine production style on airway morphology. American Journal of Respiratory and Critical Care Medicine 187:A4486.
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Progress 09/01/11 to 08/31/12
Outputs
OUTPUTS: A proteomics laboratory was established at North Carolina A&T State University through the acquisition of a GelDoc imager and associated conversion screens, a gel stainer, 2D gel electrophoresis system, isoelectric focusing system (IEF), proteomics image acquisition and analysis software. Several lectures have been developed for the purpose of introducing and teaching proteomics principles and applications to undergraduate and graduate students. Outputs from this project include four (4) lectures, two (2) seminars, two (2) proteomics protocols, one (1) thesis entitled, "Proteomic Analysis of The Impact of Management Programs on Porcine Airways" was supported, in part, by this project; and three (3) conference presentations. There were also three (3) professional development activities sponsored by this project (23 people impacted). The findings have been disseminated through a master of science thesis and a series of lectures, seminars and conference presentations. There have been a total of 11 presentations and 222 people have been impacted. In Year 1 we have impacted a total of 245 students, staff teachers and researchers though this project. PARTICIPANTS: We have worked with several students on this project, mainly graduate students to develop, optimize and implement protocols prior to using them in courses. Currently, four (4) master level students have incorporated the use of proteomics resources, tools and technologies sponsored by this grant into their thesis projects. One thesis has been completed; at least three more have ongoing activity. Several presentations (11) have been given by the PD and graduate students working on this project. There have been three (3) professional development activities provided by this project and 23 people have been impacted. A Hands-On Two-Dimensional Gel-based Proteomics Training was sponsored for LASC 660 students (PrIME grant), April 16, 18, 23, 25, 2012. Students learned how to separate and stain proteins using two-dimensional (2D) gel electrophoresis. They were also trained to use the ChemiDoc Imaging System to analyze and interpret differences in protein abundance. Lectures were used to provide the basic principles on each technique. (7 participants: 1 faculty, 1 undergraduate and 5 graduate students) A Hands-On ChemiDoc training session was held for faculty, staff and students to demonstrate proper usage of the ChemiDoc imager and accompanying image analysis software. The training was conducted in collaboration with Arielle Linderman, Proteomics Systems Specialist at Bio-Rad Laboratories on April 5, 2012. (12 people impacted: 2 faculty, 2 staff, and 3 undergraduate and 5 graduate students) The PD, Jenora Waterman, attended the "Proteomics Four-Day Hands-On Workshop" at the National Jewish Health Research Center in Denver, Colorado (September 25-28, 2012); Course Director, Dr. Nichole Reisdorph. (1 participant) In Year 1 we have impacted a total of 245 students, staff, teachers and researchers though this project. TARGET AUDIENCES: The primary target audience is undergraduate and graduate students in the disciplines of agricultural, environmental and life sciences at North Carolina A&T State University. We are also interested in targeting teaching and research faculty, and staff so that the knowledge gained will be transferred to students for years to come. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Proteomics is a relatively new branch of science with techniques that may be used in various discipline including agricultural and life sciences; however, students at minority serving institutions may have access to such resources. A proteomics laboratory was established at North Carolina Agricultural and Technical State University through the acquisition of several key pieces of equipment. The establishment of a proteomics lab has brought the discipline of proteomics to the campus of North Carolina A&T State University and given students access to leading edge instrumentation, principles and techniques. Access to leading edge technologies will provide training opportunities for minority students and produce a diverse set of leaders for the field of agriculture. We have been able to establish a proteomics laboratory at North Carolina A&T State University with gel-based proteomics capabilities. We purchased instrumentation to separate complex mixtures of proteins based on their size and charge, along with a ChemiDoc Imager and ImageLab Software for visualization and analysis of stained gels and blots. Students and faculty at North Carolina A&T State University who had not previously learned or applied proteomics technologies have gained proteomics knowledge and have the resources to apply them in the classroom and or laboratory setting. In year one, the USDA/NIFA-sponsored PrIME at North Carolina A&T State University project has impacted 245 participants through a series of presentations (222 or 90%) including proteomics-focused lectures, seminars, conferences, and hands-on training workshops (23 or 11%).
Publications
- No publications reported this period
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