Progress 07/01/10 to 01/01/14
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Hans has retired and is no longer available.
Publications
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: As indicated in our previous reports, (-)- enantiomeric compounds are intermediates in the synthesis of (+)-pisatin. Last year we concentrated on purifying the enzyme that converts the cis isomer of (-)-7,2'-dihydroxy-4',5'-methylenedioxyisoflavanol [(-)-DMDI] into an achiral intermediate (an isoflavene) that could serve as the step for the change in configuration that will ultimately produce a (+)-derivative like that of (+)-pisatin. Protein extracted from pea tissue elicited to produce (+) pisatin were also shown to produce an unidentified product from the isoflavene and efforts are underway to identify this product. PARTICIPANTS: Graduate student Rhodesia Celoy and undergraduates Jack-Mahdiehl Suazo-Siqueinros and Benjamin Gee are working on this project as is lab manager Cathy Wasmann. TARGET AUDIENCES: Our target audience are other plant biochemist and we are informing them of our work through scientific publications and participation in scientific meetings. PROJECT MODIFICATIONS: No major changes.
Impacts
The prior reseach had demonstrated that pea tissue that is synthesizing (+) pisatin converts cis (-)-DMDI into an achiral isoflavene. These same The same protein preparation also converted the isoflavene to four unidentified some of them which we believe are additional intermediates in the (+) pisatin pathway and are current effort is aimed at identifying these intermediates. The impact of our research results initially are on those studying the biosynthesis of secondary metabolites in plants.
Publications
- Coleman, J. F., G. J. White, M. Rodriguez-Carres, and H. D. VanEtten. 2011. An ABC transporter and a cytochrome P450 of Nectria haematococca MPVI are virulence factors on pea and are the major tolerance mechanisms to the phytoalexin pisatin. Mol. Plant-Microbe Inter. 24: 368-376.
- Coleman, J. J., C. C. Wasmann, T. Usami, G. J. White, E. D. Temporini, K. McCluskey, H. D. VanEtten. 2011. Characterization of the Gene Encoding Pisatin Demethylase (FoPDA1) in Fusarium oxysporum. Mol. Plant-Microbe Inter. 24: 1482-1491.
- Hawes, M., G. Curlango, F.Wen, G. J. White, H. D. VanEtten, Z. G. Xiong. 2011. Extracellular DNA: The tip of root defenses Plant Science. 180: 741-745.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: OUTPUTS: (+)-Pisatin, produced by pea (Pisum sativum L.), is an isoflavonoid derivative belonging to the pterocarpan family. (+)-Pisatin was the first chemically identified phytoalexin and subsequent research has demonstrated that most legumes produce (-)-pterocarpans with the opposite stereochemistry. Studies on the biosynthesis of (+)-pisatin had shown that (-)-enantiomeric compounds are intermediates in its synthesis. However, the step(s) from a (-)-enantiomeric intermediate to a (+)-product is still unknown. Chemical reduction of (-)-7,2'-dihydroxy-4',5'-methylenedioxyisoflavanone [(-)-sophorol], the first optically active intermediate in the (+)-pisatin pathway, produced two isomers of (-)-7,2'-dihydroxy-4',5'-methylenedioxyisoflavanol [(-)-DMDI]. NMR analysis has shown that the major product is the cis isomer and the minor product is the trans isomer. A small amount of (-)-maackiain is occasionally produced as a phytoalexin by pea along with (+)-pisatin. DISIMINATION: Graduate student Rhodesia Celoy presented her results in a poster at the American Society of Plant Biology 2010 meeting PARTICIPANTS: INDIVIDUALS: Hans VanEtten as PI, Cathy Wasmann as lab manager and Rhodesia Celoy as graduate student. COLABORATORS: Tomoyoshi Akashi from Nikon University in Japan is a colaborator on this project and spend his sabatic in our lab in 2009/2010. TARGET AUDIENCES: The Targeted Audiencs are other scientists interested in the biosynthesis of secondary metabolites in plants. How efforts to inform them of out data is through presentation at scientific meetings and publications. PROJECT MODIFICATIONS: No changes
Impacts
OUTCOME/IMPACT: We believed that (-)-DMDI is the branch point in the pathway for the production of (-)-maackiain or (+)-pisatin. Time course assays with total protein extracts from elicited pea tissue, using cis (-)-DMDI isomer as the substrate, showed the production of an achiral isoflavene. The same protein preparation also converted the isoflavene to three unidentified products and the preliminary results indicated that the trans (-)-DMDI was not metabolized by these preparations. The production of an achiral intermediate could serve as the step for the change in configuration that will ultimately produce (+)-pisatin.
Publications
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