Source: UNIVERSITY OF GEORGIA submitted to
GEORGIA NATIONAL ANIMAL HEALTH LABORATORY NETWORK PROGRAM
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0219003
Grant No.
2009-37620-05592
Project No.
GEOV-0529
Proposal No.
2010-03537
Multistate No.
(N/A)
Program Code
AA-G
Project Start Date
Aug 15, 2009
Project End Date
Aug 14, 2012
Grant Year
2011
Project Director
Allen, S. W.
Recipient Organization
UNIVERSITY OF GEORGIA
200 D.W. BROOKS DR
ATHENS,GA 30602-5016
Performing Department
College Of Vet Medicine
Non Technical Summary
The Athens Veterinary Diagnostic Laboratory and the Tifton Veterinary Diagnostic and Investigational Laboratory are active in disease surveillance, employee training and proficiency testing involving all specific animal diseases of importance to the NAHLN. Other livestock and pet animal diseases are monitored by routine accessions submitted to the diagnostic laboratories. A secure communications network within Georgia and NAHLN IT services is maintained so that veterinary diagnostic data is shared with state and federal authorities. In the past five years, laboratory staff have successfully completed all NAHLN and USDA proficiency tests and received additional training through various in-house training programs, attending regional or national meetings, and participating in short courses. Upgrades for biosafety labs to more current University regulations are being planned. NAHLN funding was partially used in the procurement of an alkaline tissue digester for environmentally safe disposal of animal material and the equipment is expected to be installed by December 2009. Computer specialists provide IT support in the transmission of records between and within the system. The directors, faculty and staff participate in statewide programs to disseminate information about disease outbreak preparedness for foreign animal diseases, agro-terrorism and food emergency response. Faculty members are involved with NAHLN working groups and in the review and establishment of AAVLD accreditation requirements. In the past five years, the Georgia laboratories have continued to develop their capabilities to respond appropriately to a severe disease outbreak. Continual funding will give the University of Georgia, Veterinary Diagnostic Laboratories the capacity to continue to detect and respond to serious disease threats including avian influenza, Newcastle disease, foot and mouth disease, classical swine fever, swine influenza, and bovine spongiform encephalopathy (BSE).
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31139991101100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3999 - Animal research, general;

Field Of Science
1101 - Virology;
Goals / Objectives
NAHLN Objectives for FY09 (1) Diagnostic testing and quality systems During FY09 - The technical staff positions that are funded through the NAHLN grant will continue to maintain and enhance disease response capability by: - Testing of diagnostic/surveillance samples received. - Continuous in-house training on real-time PCR. - Attending additional training sessions as they become available. - Participation in all available NAHLN proficiency tests. - Maintaining and enhancing the quality control/quality assurance system to ensure high- quality diagnostic results. - Participation in 2009 AAVLD quality assurance seminars (2 Directors and 2 QA/QC staff). The two laboratory Directors will continue to participate in NAHLN activities through the AAVLD and represent the laboratories within the NAHLN Methods Technical Working Group. The Tifton laboratory quality manager will attend a biosafety and biosecurity training class at the Centers for Disease Control in May 2009.The Tifton laboratory plans to obtain additional samples from small local slaughterhouses, local swine farms and feral swine for additional CSF surveillance testing. (2) Information Technology and Data Management - The IT Application Analyst will continue to be a liaison with NAHLN IT and IT specialists from other NAHLN laboratories on matters regarding proper coding and data transfer to NAHLN. The IT group at both Georgia laboratories will continue to participate in the monthly teleconferences and NAHLN quarterly IT meetings with the NAHLN laboratory group. The Tifton laboratory information system will be converted from VETLIMS to UVIS on July 1, 2009, which will allow rapid and direct transfer of results to NAHLN messaging systems. (3) Infrastructure Renovations are required in the TVDIL to upgrade BSL-3 facilities up to University specifications. Architectural design is currently in progress for enhancement of HEPA filtration and should be complete in summer 2009. NAHLN funding will be partially used in planning for the renovations.
Project Methods
The University of Georgia (UGA) Veterinary Diagnostic Laboratory (VDL) system is one of the original 12 core laboratories in the National Animal Health Laboratory Network (NAHLN) and includes two laboratories: Athens Veterinary Diagnostic Laboratory (AVDL) and Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL). The role of the Georgia VDLs in the NAHLN is to enhance the ability of the federal laboratory system to respond quickly to foreign animal disease threats. Over the last several years the Georgia laboratories have continued to develop their capabilities to respond appropriately to a severe disease outbreak. All sections in both laboratories have passed all available proficiency tests for NAHLN and other USDA program diseases (foot and mouth disease (FMD), classical swine fever, avian influenza, Newcastle disease (END), BSE, scrapie, Johne's disease, EIA and CWD on multiple animal species) in their specific disciplines and collectively have 13 proficiency tested technicians. Both laboratories participate in state and national surveillance programs for avian influenza and classical swine fever. NAHLN funding in previous years has enabled us to build our capacity to rapidly and adequately respond to severe disease outbreaks. Accomplishment reports for all years have been provided in various CRIS reports and annual renewal proposals. Continual funding will allow the Georgia laboratories to maintain capacity to detect and respond to serious disease threats and to enhance diagnostic and surveillance repertoire as validated methods and tests are developed. The Georgia laboratories have pioneered efforts in disease reporting to state and federal authorities using Rhapsody software and UVIS allowing transmission of data directly from our laboratory information systems (thus minimizing human error in this important activity).

Progress 08/15/09 to 08/14/12

Outputs
OUTPUTS: The University of Georgia (UGA) Veterinary Diagnostic Laboratory (VDL) system is made up of two laboratories: Athens Veterinary Diagnostic Laboratory (AVDL) and Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL). All sections in both laboratories have passed all available proficiency tests for NAHLN and other USDA program diseases (FMD, CSF, AI, END, BSE, scrapie, Johne's disease, EIA and CWD) and collectively have 13 proficiency tested technicians. The Georgia VDLs participate in state and national surveillance programs for AI, brucellosis, SIV, BSE, scrapie, and CSF. The AVDL and TVDIL are fully accredited by the AAVLD, through December 2012. Both laboratories have performed annual quality management reviews for each year during the project period.. The TVDIL provides testing for AI, SIV, CSF, FMD, END and PRV. The AVDL provides testing for AI, BSE, CWD, SIV, CSF, FMD, END and PRV. The TVDIL was selected to participate in both the NAHLN Pseudorabies (PRV) and Classical Swine Fever (CSF) surveillance projects in 2010 and again in 2012. Directors and QA/QC managers for both labs have participated in NAHLN training workshops through AAVLD, various webinars and teleconferences throughout the funding period. The TVDIL information system was migrated successfully from VETLIMS to UVIS on July 1, 2009. On June 1, 2010 the TVDIL was reclassified as a NAHLN member laboratory. In 2010 the TVDIL procured funding for a HEPA filter upgrade of the existing BSL3 laboratory space and construction with certification was completed in the fall of 2011. In 2009, 2010 and 2011, the two laboratory directors participated in the NAHLN directors' annual meetings at the annual AAVLD meetings, and two personnel from AVDL participated in the 2010 NAHLN Methods Technical Working Group meeting in Chicago. Currently, 7 pathology faculty members have completed the foreign animal disease training course at Plum Island. Eight technicians from both laboratories routinely participate in all NAHLN proficiency tests. A total of 11 persons from both labs have completed the current USDA online IT security training. Our LIMS application analyst pioneered the process of disease reporting to state and federal authorities using Rhapsody software and UVIS allowing data transmission directly to NAHLN from our laboratory information systems. Both labs have purchased and deployed high throughput equipment for nucleic acid extraction/processing and 7 technicians are trained on using these systems. The Georgia laboratories currently have a written agreement with the North Carolina Diagnostic Laboratory System and verbal agreements with other Southeastern states labs to provide surge testing capacity in an emergency situation. In November, 2012 both laboratories will be officially audited by the AAVLD Accreditation Committee. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The Athens Veterinary Diagnostic Laboratory (AVDL) and the Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL) have developed a regional capacity to accurately and rapidly diagnose eight USDA program diseases: AI, BSE, CWD, SIV, CSF, FMD, END and PRV. NAHLN funding has been essential to allow personnel in both labs to be proficiently trained and has provided funds for equipment and system upgrades so that the laboratories are adequately prepared in the event of a foreign animal disease or agro-terrorism disease outbreak.Past NAHLN funding has also provided partial support for the purchase of a tissue digester at the AVDL and for upgrading the TVDIL BSL-3 facility to meet current USDA/CDC specifications.In summary, NAHLN funding over the past funding period has enhanced the capabilities of the two Georgia Veterinary Laboratories in the following ways: 1) Enhanced record keeping and data transmission to state and federal authorities. 2) Enhanced preparedness to respond to any accidental or intentional foreign animal disease incidents via better trained and proficiency-tested technicians, as well as, increased capacity to test for several emerging and foreign animal diseases. 3) Enhanced overall laboratory testing capacity as a result of NAHLN networking activities (conference calls, newsletter) and the provision of standardized operating procedures from NAHLN.

Publications

  • No publications reported this period


Progress 08/15/10 to 08/14/11

Outputs
OUTPUTS: The University of Georgia (UGA) Veterinary Diagnostic Laboratory (VDL) system is made up of two laboratories: Athens Veterinary Diagnostic Laboratory (AVDL) and Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL). Both labs are members of the NAHLN. Over the last several years the Georgia laboratories have continued to develop their capabilities to respond appropriately to a severe disease outbreak. All sections in both laboratories have passed all available proficiency tests for NAHLN and other USDA program diseases (FMD, CSF, AI, END, BSE, scrapie, Johne's disease, EIA and CWD) and collectively have 12 proficiency tested technicians. The Georgia VDLs participate in state and national surveillance programs for AI, brucellosis, SIV, BSE, scrapie, and CSF. The AVDL and TVDIL are fully accredited by the AAVLD, through December 2012. Our quality system includes maintenance of standard operating procedures for all laboratory processes, training personnel on quality management, and maintaining adequate training records. In 2010, the two laboratory directors participated in the NAHLN directors' annual meeting in Minneapolis and two personnel from AVDL participated in the NAHLN Methods Technical Working Group meeting in Chicago. Four quality management staff (two from each laboratory) participated in the 2010 NAHLN-sponsored quality training workshop at Ames, IA. Four pathology faculty members have completed the foreign animal disease training course at Plum Island. Technicians at both laboratories routinely participate in all NAHLN proficiency tests. A total of 13 persons (8 from AVDL and 5 from TVDIL) have completed the current USDA online IT security training. Our application analyst has developed a Rhapsody-based interface for transmitting test results directly from our LIMS (UVIS) to the NAHLN database. Messaging using this interface is now the routine method for transmitting avian influenza and BSE test results to NAHLN. We continue to transmit monthly disease reports to the VSLS database, as well as state reportable disease data to the Georgia Department of Agriculture. Recently, the University of Georgia, College of Veterinary Medicine became the official vendor for our LIMS, which has been re-named VetView. In the last few years, both laboratories have purchased and deployed high throughput equipment for nucleic acid extraction and preparation of PCR master mixes. Currently, 7 technicians (3 at AVDL and 4 at TVDIL) are trained on using these high throughput systems. One technician from the AVDL attended the NAHLN-sponsored high throughput training course in Manhattan, KS in 2010. Currently the Georgia laboratories have a written agreement with North Carolina and verbal agreements with other Southeastern states labs to provide surge testing capacity in an emergency situation. We are members of the FERN and VetLRN. Also in 2010 the TVDIL was reclassified as a NAHLN member laboratory and was selected to participate in the NAHLN Pseudorabies (PRV) surveillance project. Implementation of a HEPA filter upgrade of the existing BSL3 laboratory space at the TVDIL is currently in progress. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The Athens Veterinary Diagnostic Laboratory (AVDL) and the Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL) have developed a regional capacity to accurately and rapidly diagnose eight USDA program diseases: AI, BSE, CWD, SIV, CSF, FMD, END and PRV. NAHLN funding has allowed personnel in both labs to be proficiently trained and has provided funds for equipment and system upgrades so that the laboratories are optimally prepared in the event of a foreign or agro-terrorism disease outbreak. Staff from both laboratories have continued to participate in all available proficiency testing programs. NAHLN funding has provided partial support for the purchase of a tissue digester and for upgrading one BSL-3 facility to meet USDA/CDC specifications. In summary, NAHLN funding over the past six years has enhanced the capabilities of the two Georgia Veterinary Laboratories in the following ways: 1) Enhanced record keeping and data transmission to state and federal authorities. 2) Enhanced preparedness to respond to any accidental or intentional foreign animal disease incidents resulting in better trained and proficiency-tested technicians, as well as, increased capacity to test for several emerging and foreign animal diseases. 3) Enhanced overall laboratory capacity as a result of NAHLN networking activities (conference calls, newsletter) and the supply of standard operating procedures from NAHLN.

Publications

  • No publications reported this period


Progress 08/15/09 to 08/14/10

Outputs
OUTPUTS: The Athens Veterinary Diagnostic Laboratory (AVDL) and the Tifton Veterinary Diagnostic and Investigational Laboratory (TVDIL) have developed a regional capacity to accurately and rapidly diagnose eight USDA program diseases: AI, BSE, CWD, SIV, CSF, FMD, END and PRV. NAHLN funding provided partial support for the purchase of a tissue digester and for upgrading one BSL-3 facility to meet USDA/CDC specifications. The remaining $150K funding for the TVDIL BSL-3 HEPA upgrade has been procured and the upgrade should be completed in fall 2010. The TVDIL was selected to participate in the current NAHLN Pseudorabies (PRV) surveillance project effective September 1, 2010. During FY09, the two labs began transmitting monthly disease reports to the VSLS database and continued to transmit reportable disease data to the Georgia Dept. of Agriculture.The TVDIL information system was migrated successfully from VETLIMS to UVIS on July 1, 2009 making it much easier for reporting results using the NAHLN IT system. In 2010 three technicians from AVDL and four from TVDIL successfully completed NAHLN proficiency testing for AI, SIV, and NDV. On June 1, 2010 the TVDIL was reclassified as a NAHLN member laboratory. Directors, lab QA/QC managers and virology staff for both labs routinely participated in NAHLN activities through AAVLD, NAHLN webinars and teleconferences in FY10. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
NAHLN funding has allowed personnel in both labs to be proficiently trained and has provided funds for equipment and system upgrades so that the laboratories are optimally prepared in the event of a foreign or agro-terrorism disease outbreak. Staff from both laboratories have continued to participate in all available proficiency testing programs. The NAHLN IT messaging system is working seamlessly in submitting data from the laboratories to the NAHLN database.

Publications

  • No publications reported this period