Progress 10/01/08 to 09/30/12
Outputs
OUTPUTS: The outcome of this project was genotypic data, performance evaluations of maize germplasm, and knowledge that will facilitate breeding plant breeding to improve maize for use in lignocellulosic biofuel production. Genetic data was developed and analyzed using a targeted SNP genotyping platform and by targeted genome and exome sequencing. In total, more than 1 million positions were scored on a panel of 700 maize inbred lines. Inbred lines and their crosses were evaluated in Arlington, WI in 2010 to 2012. Association analysis and comparison of grain and stover yield was conducted. PARTICIPANTS: James Johnson is the graduate research assistant conducting the research for this project. TARGET AUDIENCES: The target audience for this study is geneticists, plant breeders, and physiologists working on improving crops for biomass traits. This target audience will be reached through presentations at national meetings and via scientific publication. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Genetic diversity within the set of inbred lines used (available at the North Central Region Plant Introduction station) were characterized using a defined set of single-nucleotide polymorphism (SNP) markers (Hansey et al. 2011) and genome-wide markers (publication in preparation). Association analysis to detect genotype/phenotype relationships in diverse hybrids versus inbreds and testcrosses allows the effect of all three genotypic states to be determined. Random crossing among inbreds to produce hybrids does not resolve population structure found within the inbred parental set. Statistical approaches to association analysis in diverse hybrids, and strengths and weaknesses of the approach have been determined (publication in preparation). In the comparison of grain versus stover yield, we determined that there is substantial variation in stover yield that is only weakly correlated with grain yield indicating the potential to breed high-yielding grain hybrids that have improved stover yield, and supporting the idea that heterosis is not of the same magnitude for each trait in a cross, but is trait specific.
Publications
- Muttoni, G., J.M. Johnson, N. Santoro, C.J. Rhiner, S.M. Kaeppler, and N. de Leon. 2012. A high-throughput tissue core sampling device for the evaluation of biomass composition. Biotechnology for Biofuels 5:27. doi:10.1186/1754-6834-5-27.
- Kaeppler, S.M. 2012. Heterosis: Many genes, many mechanisms - stop the search for an undiscovered unifying theory. ISRN Botany Article ID 682824 doi:10.5402/2012/682824.
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: Performance data were gathered to address the hypothesis that heterotic patterns are non-overlapping for grain-yield and stover traits. Performance data were gathered to empirically assess genetic interpretations from genome-wide association analysis when materials are evaluated as crosses to one tester versus random testers. Association analysis is proceeding on biomass yield and component traits. PARTICIPANTS: James Johnson, Ph.D. student TARGET AUDIENCES: The target audience for this study is geneticists, plant breeders, and physiologists working on improving crops for biomass traits. This target audience will be reached through presentations at national meetings and via scientific publication. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We are in the process of analyzing data that have been gathered in years 1 to 3, but it has not yet been synthesized into specific outcomes.
Publications
- Hansey, C.N., J.M. Johnson, R.S.Sekhon, S.M. Kaeppler, and N. de Leon. 2011. Genetic diversity of a maize association population with restricted phenology. Crop Sci 51: 2: 704-715.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: Performance data were gathered to address the hypothesis that heterotic patterns are non-overlapping for grain-yield and stover traits. Performance data were gathered to empirically assess genetic interpretations from genome-wide association analysis when materials are evaluated as crosses to one tester versus random testers. PARTICIPANTS: James Johnson is the graduate student conducting the research for this project. TARGET AUDIENCES: The target audience for this study is geneticists, plant breeders, and physiologists working on improving crops for biomass traits. This target audience will be reached through presentations at national meetings and via scientific publication. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We are in the process of analyzing data that have been gathered in years 1 and 2, but it has not yet been synthesized into specific outcomes.
Publications
- Johnson, J.M., S. Kaeppler, and N. de Leon. 2010. Approaches to association analysis in a hybrid context. ASA, CSSA, and SSSA 2010 International Meetings, Oct. 31-Nov. 4, 2010. Long Beach, CA.
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Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: The first season of field trials for this experiment was conducted at Arlington, WI with a replicated evaluation of approximately 300 hybirids from a diallel mating design. Data for flowering and plant height were collected. Due to an exceptionally cool fall, data was only collected on a subset of hybrids for stover and grain yield. Recombinant inbred line development continued for phase II materials. PARTICIPANTS: James Johnson is the graduate student conducting the research for this project. TARGET AUDIENCES: The target audience for this study is geneticists, plant breeders, and physiologists working on improving crops for biomass traits. This target audience will be reached through presentations at national meetings and via scientific publication. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
As we are still early in this project, the results have not yet been synthesized into outcomes.
Publications
- No publications reported this period
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Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: This project has just been initiated, and outputs are not yet available. PARTICIPANTS: James Johnson is the graduate student who will work on this project. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Plans and preparations are under way for the 2009 field season. Graduate student James Johnson has been recruited to conduct the research and is receiving training in plant breeding.
Publications
- No publications reported this period
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