Progress 07/01/07 to 06/30/12
Outputs
OUTPUTS: Michigan State University serves as the hub for the North Central Plant Diagnostic Network (NCPDN) and coordinates diagnostic and training efforts in the region. The NCPDN consists of members from Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. During this cooperative agreement, training to enhance diagnostic ability and diagnostic enhancement were emphasized. Funding supported attendance at training sessions/meetings at professional society meetings, the Third NPDN Meeting,the University of Kentucky's real time PCR workshop, and APHIS-PPQ-CPHST training sessions. Diagnosticians also attended several webinar training opportunities on subjects including boxwood blight, measuring impacts, and rust fungi. The NCPDN sponsored a two day nematology workshop held at Michigan State University. The workshop was attended by diagnosticians from the north central and north east regions and emphasized hands-on-training. The 2012 NCPDN regional meeting was held at the Ohio Department of Agriculture where the ODA and Ohio State University diagnostic laboratories are co-located. The meeting included sessions on scale insects, emerald ash borer and Asian longhorn beetle, lab accreditation in the animal disease diagnostic community, and training on a technique for immunocapture RT-PCR and cDNA synthesis from dsRNA templates. The attendees examined specimens of Boxwood with signs and symptoms of Boxwood blight. As is done every year, each state discussed what was new in terms of pests and diseases. All NDPCN diagnostic labs enter diagnostic sample results into the NPDN National Data Repository (NDR). Between July 1, 2011 and June 30, 2012, 30,098 diagnostic records were entered into the NDR. During the entire cooperative agreement (July 1, 2007-June 30, 2012) 113,488 records were entered. The number of records entered increased in year. Several diagnostic labs in the north central region participated in surveys for P. ramorum and other pests and pathogens. Approximately 450 samples were collected and tested for the presence of P. ramorum. Michigan State University's Diagnostic Services lab received and tested 108 P. ramorum suspect samples from states within the region. Samples were tested at MSU using the USDA APHIS PPQ real-time PCR protocol. Several labs participated in nematode surveys. Diagnosticians in Illinois and Michigan processed samples 2600 and 1100, respectively, for soybean cyst nematode quantification. Michigan also processed 1030 samples for a potato cyst nematode survey. Our region used a variety of media platforms to deliver over 220 presentations. Presentations were made to Master Gardeners, county educators, school groups, graduate students, and growers. First detector awareness and first detector training sessions were held in Illinois, Indiana, Missouri, Ohio and Wisconsin. Over 45 training sessions were held, training approximately 3,000 participants. PARTICIPANTS: Ray Hammerschmidt is the PI for this project. He provided direction for the overall operations of the NCPDN as the regional director and served as associate executive director of the NPDN. Janet Byrne served as the Associate Director or the NCPDN and lead diagnostician for the region, assisted in NCPDN activities and helped manage regional diagnostics activities. She served as liaison to both diagnosticians in the region and nationally. Lee Duynslager served as the NCPDN Information Technologist and assisted in the implementation of the distributed network at NCPDN and to assist NCPDN member institutions. He also served as the liaison with other IT specialists in the national and regional networks. Amy Dunfee served as training coordinator. Other participants include Laura Jesse (Iowa State University), Suzanne Bissonette (University of Illinois), Gail Ruhl and Tom Creswell (Purdue University), Adam Leonberger (University of Missouri), Dimitre Mollov (University of Minnesota), Nancy Taylor (Ohio State University) and Brian Hudelson (University of Wisconsin). NCPDN members were also engaged in many of the NPDN national committees. TARGET AUDIENCES: Land grant and state department of agriculture diagnosticians, extension educators, extension specialists, growers, master gardeners, and crop consultants that are located in the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. These groups were reached through formal workshops, first detector training programs, extension presentations on diagnostics and new/emerging disease and pest problems, and by providing diagnostic education and services to clientele groups PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Diagnosticians in the NCPDN were provided resources and training opportunities to improve their diagnostic skills for diseases and pests and to enhance needed diagnostics infrastructure. This is clearly seen in the numbers of samples processed and records submitted to the National Repository. Several new pests were identified during the last year and diagnostic infrastructure is in place to reduce the risk from Ramorum blight and other significant pests and pathogens through rapid diagnostics and communication through proper channels. NCPDN members have been involved in the development of a lab accreditation system which will enhance our abilities to diagnose problems. Training of first detectors continued and this program serves to aid in our abilities for early detection of pests and pathogens. We have also reached a large number of stakeholders through programming and written materials. Diagnostic equipment improvements were achieved in several NCPDN labs. Communication among the NCPDN land grant diagnosticians and stakeholders was enhanced.
Publications
- No publications reported this period
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Progress 07/01/10 to 06/30/11
Outputs
OUTPUTS: The regional center at Michigan State University serves as the hub for the North Central Plant Diagnostic Network (NCPDN) and serves to coordinate diagnostic and training efforts in the region. The NCPDN consists of members from the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. Emphasis was put on training as a means to enhance diagnostic ability. NCPDN diagnosticians attended a range of training sessions/meetings including the Entomological Society of America and American Phytopathology Society annual meetings, National Plant Diagnostic Network IT/Diagnostician Meeting, Thousand Cankers Disease Forestry Conference, Diagnostic Techniques Workshop, Fusarium Identification workshop, and the Plant-Parasitic Nematode Identification Short Course. Training sessions coordinated by APHIS-PPQ-CPHST for Phytophthora ramorum, PPV and Bioinformatics were also attended by NCPDN diagnosticians. The NCPDN sponsored a four day virology workshop at the University of Minnesota. Nine NCPDN diagnosticians attended. The workshop emphasized hands-on-training and included a variety of techniques (PCR, electron microscopy, etc). Another major emphasis was on diagnostics and data entry. The NDPCN diagnostic labs entered diagnostic sample results were uploaded to the NPDN National Data Repository (NDR). Data from over 19,500 diagnostic samples were submitted to the NDR between July 1, 2010 and June 14, 2011. Several diagnostic labs in the north central region participated in surveys for Phytophthora ramorum and other pests and pathogens. Approximately 450 samples were collected and tested, for the presence of P. ramorum. Michigan State University's lab tested 234 P. ramorum suspect samples from states within the region. Samples were tested at MSU using the USDA APHIS PPQ real-time PCR protocol. Two samples, from two states in the region, tested positive for P. ramorum. The samples were submitted as part of a trace forward investigation. Labs in Illinois, Iowa, and Indiana processed 1354 samples for phytosanitary purposes. Several states in the region had first detections of the brown marmorated stink bug, spotted winged drosophila, and basil downy mildew. In April 2011, the NCPDN annual meeting was hosted by the Iowa State University diagnostic lab. The meeting included diagnostic information on tick biology and identification, corn and soybean disease diagnostics, the many causes of flyspeck and sooty blotch of apple and an update on Burr Oak Blight that included some hands on activities. NCPDN members delivered over 250 presentations and/or publications. Presentations were made to Master Gardeners, county educators, school groups, graduate students, and growers. Diagnosticians at Ohio State and the University of Wisconsin regularly participate in a radio talk show educating thousands in the general public about current disease and pest issues. Eighty first detector awareness and first detector training sessions were held. Diagnosticians from every NCPDN state gave presentations in Master Gardener training programs. Some diagnosticians in the region are using web based methods to provide training for a range of audiences. PARTICIPANTS: Ray Hammerschmidt is the PI for this project. He provided direction for the overall operations of the NCPDN as the regional director and served as associate executive director of the NPDN. Janet Byrne served as lead diagnostician for the region, assisted in NCPDN activities and helped manage regional diagnostics activities. She served as liaison to both diagnosticians in the region and nationally. Lee Duynslager served as the NCPDN Information Technologist and assisted in the implementation of the distributed network at NCPDN and to assist NCPDN member institutions. He also served as the liaison with other IT specialists in the national and regional networks and maintains the NCPDN web page. Amy Peterson joined the NCPDN as training coordinator. Other participants include Laura Jesse (Iowa State University), Suzanne Bissonette (University of Illinois), Gail Ruhl and Tom Creswell (Purdue University), Adam Leonberger (University of Missouri), Dimitre Mollov (University of Minnesota), Nancy Taylor (Ohio State University) and Brian Hudelson (University of Wisconsin). NCPDN members were also engaged in many of the NPDN national committees. TARGET AUDIENCES: Land grant and state department of agriculture diagnosticians, extension educators, extension specialists, growers, master gardeners, and crop consultants that are located in the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. These groups were reached through formal workshops, first detector training programs, extension presentations on diagnostics and new/emerging disease and pest problems, and by providing diagnostic education and services to clientele groups PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Diagnosticians in the NCPDN were provided resources and training opportunities to improve their diagnostic skills for diseases and pests and to enhance needed diagnostics infrastructure. This is clearly seen in the numbers of samples processed and records submitted to the National Repository. Several new pests were identified during the last year and diagnostic infrastructure is in place to reduce the risk from Ramorum blight and other significant pests and pathogens through rapid diagnostics and communication through proper channels. NCPDN members have been involved in the development of a lab accreditation system which will enhance our abilities to diagnose problems. Training of first detectors continued and this program serves to aid in our abilities for early detection of pests and pathogens. We have also reached a large number of stakeholders through programming and written materials. Diagnostic equipment improvements were achieved in several NCPDN labs. Communication among the NCPDN land grant diagnosticians and stakeholders was enhanced.
Publications
- 2010/07 TO 2011/06 DiFonzo, C., Russell, H. 2010. Noctua pronuba (Lepidoptera: Noctuidae): An Outbreak in Emails. Journal of Integrated Pest Management 1: 1-6.
- Ruhl, G., Twieg, E., DeVries, R., Levy, L., Byrne, J., Mollov, D., Taylor, N. 2011. First report of bacterial wilt in Mandevilla splendens Red Riding Hood in the United States Caused by Ralstonia solanacearum biovar 3. Plant Disease 95: 614.
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Progress 07/01/09 to 06/30/10
Outputs
OUTPUTS: The regional center at Michigan State University serves as the hub for the North Central Plant Diagnostic Network (NCPDN) and consists of members from the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. Emphasis was put on training as a means to enhance diagnostic ability. Funding supported diagnostician attendance at a range of training sessions/meetings including, the National Turfgrass Entomology Workshop, Adult Lepidoptera Identification Workshop, American Phytopathology Society annual meeting, National Ornamentals Workshop, Real Time Workshop for Applied Plant Pathologists, and Macroarray Membrane Hybridization Training. APHIS-PPQ-CPHST training for Phytophthora kernovae and P. ramorum were also attended by diagnosticians from the NCPDN. In the time period of July 1, 2008 through June 30, 2009 our members diagnosed over 17,000 samples. The NDPCN diagnostic labs entered diagnostic sample results into the PDIS database system, and these data are subsequently uploaded to the NPDN National Data Repository (NDR). Data from 16,111 samples were submitted to the NDR. These numbers differ due to the timing of uploads to the NDR (samples may have been submitted but not completed or uploaded yet) and due to the type of sample. Illinois, Indiana, and Michigan recorded first reports for pathogens not previously reported in their state. These included Goss's Wilt (Clavibacter michiganense subsp. nebraskensis) on field and sweet corn, and Southern Wilt (Ralstonia solancearum) on Mandevilla. Over 400 samples were collected and tested, all tested negative for the presence of P. ramorum. In April 2009, the NCPDN annual meeting was hosted by the University of Missouri's diagnostic lab. The meeting included hands-on training in the identification of insect pests of stored grain, and sessions on turf diagnostics, the impacts of spring ice damage on woody species, the biology and diagnosis of Armillaria, and mushroom identification. NCPDN members delivered over 250 presentations and/or publications. Presentations were made to Master Gardeners, county educators, school groups, graduate students, and growers. Diagnosticians at Ohio State and the University of Wisconsin regularly participate in a radio talk show educating thousands in the general public about current disease and pest issues. A total of 25 First detector awareness and first detector training sessions were held reaching 1551 participants. Diagnosticians from every NCPDN state gave presentations in Master Gardener training programs. Some diagnosticians in the region are using web based methods to provide training for a range of audiences. At Purdue, publications were created and regional training on Goss's wilt was conducted. The detection of Goss's was communicated to other diagnosticians in the NCPDN leading to further detections of this disease in the region. Michigan State University's Diagnostic Services lab, in conjunction with the Entomology Department led a two day hands-on insect identification workshop for extension educators and other office staff. Participants were taught basic insect identification skills. PARTICIPANTS: Ray Hammerschmidt is the PI for this project. He provided direction for the overall operations of the NCPDN as the regional director and served as executive director of the NPDN. Janet Byrne served as lead diagnostician for the region, assisted in NCPDN activities and helped manage regional diagnostics activities. She served as liaison to both diagnosticians in the region and nationally. Lee Duynslager served as the NCPDN Information Technologist and assisted in the implementation of the distributed network at NCPDN and to assist NCPDN member institutions. He also served as the liaison with other IT specialists in the national and regional networks and maintains the NCPDN web page. Janette Jacobs coordinated diagnostic training support for the NCPDN and assisted diagnosticians in the regions with new diagnostic tools. Other participants include Laura Jesse (Iowa State University), Nancy Pataky (University of Illinois), Gail Ruhl and Tom Creswell (Purdue University), Simeon Wright (University of Missouri), Dimetre Molov (University of Minnesota), Nancy Taylor (Ohio State University) and Brian Hudelson (University of Wisconsin). TARGET AUDIENCES: Land grant and state department of agriculture diagnosticians, extension educators, extension specialists, growers, master gardeners, and crop consultants that are in the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. These groups were reached through formal workshops, first detector training programs, extension presentations on diagnostics and new/emerging disease and pest problems, and by providing diagnostic education and services to clientele groups. PROJECT MODIFICATIONS: A new annual plan of work was developed in compliance with the NPDN Strategis Plan and Project Planning Matrix.
Impacts
Diagnosticians in the NCPDN were provided resources and training opportunities to improve their diagnostic skills for diseases and pests and to enhance needed diagnostics infrastructure. Diagnostic equipment improvements were achieved in several NCPDN labs. Specific training in detection and notification was utilized during detections of possible high-risk pathogens in three states. Communication among the NCPDN land grant diagnosticians and identifiers was enhanced.
Publications
- Publications can be found on the regional (www.ncpdn.org) and national (www.npdn.org) websites and links at each site. 2009
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Progress 07/01/08 to 06/30/09
Outputs
OUTPUTS: The regional center at Michigan State University serves as the hub for the North Central Plant Diagnostic Network (NCPDN) and consists of members from the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. Emphasis was put on training as a means to enhance diagnostic ability. Funding supported diagnostician attendance at a range of training sessions/meetings including, the National Turfgrass Entomology Workshop, Adult Lepidoptera Identification Workshop, American Phytopathology Society annual meeting, National Ornamentals Workshop, Real Time Workshop for Applied Plant Pathologists, and Macroarray Membrane Hybridization Training. APHIS-PPQ-CPHST training for Phytophthora kernovae and P. ramorum were also attended by diagnosticians from the NCPDN. In the time period of July 1, 2008 through June 30, 2009 our members diagnosed over 17,000 samples. The NDPCN diagnostic labs entered diagnostic sample results into the PDIS database system, and these data are subsequently uploaded to the NPDN National Data Repository (NDR). Data from 16,111 samples were submitted to the NDR. These numbers differ due to the timing of uploads to the NDR (samples may have been submitted but not completed or uploaded yet) and due to the type of sample. Illinois, Indiana, and Michigan recorded first reports for pathogens not previously reported in their state. These included Goss's Wilt (Clavibacter michiganense subsp. nebraskensis) on field and sweet corn, and Southern Wilt (Ralstonia solancearum) on Mandevilla. Over 400 samples were collected and tested, all tested negative for the presence of P. ramorum. In April 2009, the NCPDN annual meeting was hosted by the University of Missouri's diagnostic lab. The meeting included hands-on training in the identification of insect pests of stored grain, and sessions on turf diagnostics, the impacts of spring ice damage on woody species, the biology and diagnosis of Armillaria, and mushroom identification. NCPDN members delivered over 250 presentations and/or publications. Presentations were made to Master Gardeners, county educators, school groups, graduate students, and growers. Diagnosticians at Ohio State and the University of Wisconsin regularly participate in a radio talk show educating thousands in the general public about current disease and pest issues. A total of 25 First detector awareness and first detector training sessions were held reaching 1551 participants. Diagnosticians from every NCPDN state gave presentations in Master Gardener training programs. Some diagnosticians in the region are using web based methods to provide training for a range of audiences. At Purdue, publications were created and regional training on Goss's wilt was conducted. The detection of Goss's was communicated to other diagnosticians in the NCPDN leading to further detections of this disease in the region. Michigan State University's Diagnostic Services lab, in conjunction with the Entomology Department led a two day hands-on insect identification workshop for extension educators and other office staff. Participants were taught basic insect identification skills. PARTICIPANTS: Ray Hammerschmidt is the PI for this project. He provided direction for the overall operations of the NCPDN as the regional director and served as executive director of the NPDN. Janet Byrne served as lead diagnostician for the region, assisted in NCPDN activities and helped manage regional diagnostics activities. She served as liaison to both diagnosticians in the region and nationally. Lee Duynslager served as the NCPDN Information Technologist and assisted in the implementation of the distributed network at NCPDN and to assist NCPDN member institutions. He also served as the liaison with other IT specialists in the national and regional networks and maintains the NCPDN web page. Janette Jacobs coordinated diagnostic training support for the NCPDN and assisted diagnosticians in the regions with new diagnostic tools. Other participants include Laura Jesse (Iowa State University), Nancy Pataky (University of Illinois), Gail Ruhl and Tom Creswell (Purdue University), Simeon Wright (University of Missouri), Dimetre Molov (University of Minnesota), Nancy Taylor (Ohio State University) and Brian Hudelson (University of Wisconsin). TARGET AUDIENCES: Land grant and state department of agriculture diagnosticians, extension educators, extension specialists, growers, master gardeners, and crop consultants that are in the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. These groups were reached through formal workshops, first detector training programs, extension presentations on diagnostics and new/emerging disease and pest problems, and by providing diagnostic education and services to clientele groups. PROJECT MODIFICATIONS: A new annual plan of work was developed in compliance with the NPDN Strategic Plan and Project Planning Matrix.
Impacts
Diagnosticians in the NCPDN were provided resources and training opportunities to improve their diagnostic skills for diseases and pests and to enhance needed diagnostics infrastructure. Diagnostic equipment improvements were achieved in several NCPDN labs. Specific training in detection and notification was utilized during detections of possible high-risk pathogens in three states. Communication among the NCPDN land grant diagnosticians and identifiers was enhanced.
Publications
- Publications can be found on the regional (www.ncpdn.org) and national (www.npdn.org) websites and links at each site. 2009
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Progress 07/01/07 to 06/30/08
Outputs
OUTPUTS: The regional center at Michigan State University serves as the hub for the North Central Plant Diagnostic Network (NCPDN) and consists of members from the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Ohio and Wisconsin. During the reporting period, the NCPDN held two regional meetings. The first meeting was dedicated to building interactions between land grant university diagnosticians and their partners in state department of agriculture labs. The second meeting consisted of hands-on exercises and presentations on disease and pest diagnostics focusing on dsRNA extraction, split bark problems in trees, using phenology to determine insect emergence, and diagnosing insect problems on turf, vegetables and fruit. The participants also discussed the teaching of diagnostics and shared information on diseases and pests in each of their states. In January 2007, over 200 people attended the first national diagnosticians meeting which detailed progress made since the inception of the NPDN in 2002. The meeting also provided a platform for developing a vision for the future of the NPDN. The meeting included an external review of the network. The review document has generated a new strategic plan for the NPDN which is now being implemented in the NCPDN and all other regions. NCPDN diagnosticians have been engaged in diagnostics and data uploads for the ipmPIPE programs on soybean rust and legume diseases. The NCPDN provided resources to enable testing for pathogens and insects of regulatory concern and/or special interest and to support first detector training in the region. The NCPDN co-hosted multi-day workshops on bark beetles and corn nematodes. Many of the NCPDN diagnosticians have also participated in USDA-APHIS diagnostics training and conducted first detector training and given presentations on diagnostics and the importance of the NPDN for agricultural security. During the reporting period, over 20,000 diagnostic reports were uploaded to the national data repository from the NCPDN labs. The NCPDN has also provided materials and information needed for public relations efforts. PARTICIPANTS: Ray Hammerschmidt is the PI for this project. He provided direction for the overall operations of the NCPDN as the regional director and served as executive director of the NPDN. Janet Byrne served as lead diagnostician for the region, assisted in NCPDN activities and helped manage regional diagnostics activities. She served as liaison to both diagnosticians in the region and nationally. Cassandra Bates served as the training, education and exercise coordinator for the NCPDN and was responsible for coordinating training and exercises across the region. Lee Duynslager served as the NCPDN Information Technologist and assisted in the implementation of the distributed network at NCPDN and to assist NCPDN member institutions. He also served as the liaison with other IT specialists in the national and regional networks and maintains the NCPDN web page. Janette Jacobs coordinated diagnostic training support for the NCPDN and assisted diagnosticians in the regions with new diagnostic tools. Gary Parsons provided entomology information support and was engaged in developing diagnostics education materials. The NCPDN also provided first detector training for extension educators and others in the region. Workshops on corn nematode and bark beetle identification were held. These were attended by land grant university diagnosticians, state department of agriculture diagnosticians and USDA-APHIS staff members. TARGET AUDIENCES: Land grant and state department of agriculture diagnosticians, extension educators, extension specialists, growers, master gardeners, and crop consultants. These groups were reached through formal workshops (nematode and bark beetles in the last year), first detector training programs, extension presentations on diagnostics and new/emerging disease and pest problems, and by providing diagnostic education and services to clientele groups. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
2007/07 TO 2008/06 Diagnosticians in the NCPDN were provided resources and training opportunities to improved their diagnostic skills for diseases and pests and to enhance needed diagnostics infrastructure. Communication among the NCPDN land grant diagnosticians and their state department of agriculture colleagues was enhanced.
Publications
- 2007/07 TO 2008/06 recent information can be obtained at www.ncpdn.org
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