Source: WASHINGTON STATE UNIVERSITY submitted to NRP
POPULATION CONTROL OF ALFALFA LOOPER AND CORN EARWORM (LEPIDOPTERA: NOCTUIDAE) THROUGH FEMALE ANNIHILATION TECHNIQUE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0195305
Grant No.
2002-34103-12124
Cumulative Award Amt.
(N/A)
Proposal No.
2002-04090
Multistate No.
(N/A)
Project Start Date
Jul 1, 2002
Project End Date
Jun 30, 2005
Grant Year
2002
Program Code
[QQ]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
ENTOMOLOGY
Non Technical Summary
Currently, only male attracting pheromone lures are available for insect monitoring and control. Female feeding attracting lures should be more efficient in controlling pest populations because the removal of the female moth should reduce eggs laid and numbers of larvae in subsequent generations. We propose to develop chemical lures for pestiferous male and female moths, including alfalfa looper and corn earworm, based on feeding attractants.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2111480113010%
2111510113010%
2111640113010%
2113110113010%
2113199113010%
2161480113010%
2161510113010%
2161640113010%
2163110113010%
2163199113010%
Goals / Objectives
Determine reductions in adult moth activity in response to several densities of lure & kill stations, for both alfalfa looper & corn earworm moths. Achieving this objective will provide validation that a significant proportion of moths present can be removed using feeding attractants. This will also indicate what density of lure and kill stations are necessary to achieve this objective, defining a necessary parameter for progressing to the next experiment. Determine reductions in larval densities of alfalfa looper and corn earworm in fields of alfalfa and corn, effected by lure & kill stations. Achieving this objective will test the hypothesis that reductions in larvae present will be a direct result of removal of numbers of female moths. 3. Determine the seasonal pattern of response and characterize the reproductive state of female alfalfa looper and corn earworm moths attracted to the selected feeding attractants and to sex pheromone lures. Information on which moths are responding to feeding attractants through the season might be important to the further development of baiting strategies, if there are any major shifts in the demographics of moths attracted to the lures at different times of the year. Such changes might occur for example in response to alternate food sources or to environmental cues affecting migrational behavior.
Project Methods
Determine reductions in moth activity The general approach to demonstrating adult moth kill with bait stations or traps will be to monitor adult activity with a sampling method differing from that used in the bait stations and to look for differences in moth activity related to the application of bait stations. Those differences are expected to occur across time (compare before and after bait station application) and across space (compare plots treated with bait stations versus control plots). This approach will be used to assess three different densities of stations with feeding attractant for alfalfa looper, and three different densities of stations with feeding attractant for corn earworm. Determine reductions in larval densities Comparisons will be made of numbers of alfalfa looper moths captured in blacklight traps, both before and after station placement, and between control and treated plots, to confirm a strong reduction in moth activity with station placement. Additionally, mean numbers of larvae per sweep will be used to test the hypothesis that reductions in female alfalfa looper moths active in plots should result in comparable reductions in larvae on the crop. Comparisons will be made of numbers of larvae and of the demographics of larvae sampled (based on instars) both before and after station placement and between treatment and control plots. It is recognized however that a time lag is expected between a reduction in numbers of ovipositing moths in the plot and any reduction in numbers of larvae in sweep net samples. Determine the seasonal response and characterize reproductive status. Four sets of Universal moth traps (UniTraps) will be set up and maintained throughout the 2002 through 2004 field seasons, to obtain a data base on patterns of response and to obtain moths for dissections. These four sets of traps will be baited with 1) a commercial sex pheromone lure for the alfalfa looper, 2) the alfalfa looper feeding attractant comprised of phenylacetaldehyde and the Oregon grape chemical, 3) a commercial sex pheromone lure for the corn earworm, and 4) the corn earworm feeding attractant comprised of phenylacetaldehyde and methyl-2-methoxy benzoate. Five sets of these 4 traps will be set up in early April and will be maintained into late October. Traps will be checked weekly early in the season and twice weekly during periods of increased moth activity. Lures and Vaportape will be replaced every 2 weeks. Female moths captured will be dissected to determine mating and reproductive status, following the system used by Hitchcox (2000) for the Lacanobia fruitworm.

Progress 07/01/02 to 06/30/05

Outputs
Trapping female moths with feeding attractants: Both sexes of alfalfa looper (Autographa californica) and corn earworm (Helicoverpa zea) were captured with Universal Moth Traps baited with double component floral chemical lures. These experiments were conducted in a corn-growing region of central Washington State during the 2003 and 2004 seasons. Female and male corn earworm moths were captured in traps baited with phenylacetaldehyde and methoxy-2-methyl-benzoate. Lures were dispensed with a polypropylene vial that provided a controlled release rate for an extended period of time. Similarly, male and female alfalfa looper moths were captured with traps baited with phenylacetaldehyde and beta-myrcene. These experiments were conducted in the Yakima Valley during the 2003 and 2004 seasons. For each male H. zea captured, more than one male was taken. With alfalfa looper, the number of females collected increased in direct proportion with the release rate of the vials. Lures demonstrated a great potential for use in the attraction and capture of female moths. Attraction in wind tunnels: Both sexes of alfalfa looper adults were attracted (upwind flight with a zigzagging pattern) to a floral double-component chemical lure. Female moths were attracted to phenylacetaldehyde and beta-myrcene. Female alfalfa looper moths contacted the killing station 60.0% of the time. A mortality ratio of 84% (in contacted moths) was used when the killing station was loaded with Permethrin. Developing an attract and kill system: A killing station is currently being tested in the field for use in combination with lures. Field experiments are demonstrating significant reductions of female alfalfa looper on plots treated with bait stations when compared to controls. Alfalfa looper females were significantly reduced on plots treated with 50 killing stations/acre. Studies to demonstrate reduction in eggs and next generation activity are currently being conducted.

Impacts
We have shown that both male and female alfalfa looper and corn earworm moths are attracted to the floral baits phenylacetaldehyde and methoxy-2-methyl-benzoate (corn earworm) and phenylacetaldehyde and beta-myrcene (alfalfa looper). Field experiments have shown that there is a reduction in female capture when the floral baits are used in conjunction with pesticide treated killing stations. This results in a lessening of next generation eggs and larvae. If bait stations (which use small amounts of pesticide) are found to reduce numbers below economic levels, then they can be employed to drastically reduce the amount of pesticide applied to the field. Once attractants are delineated, the method should be applicable to numerous species of pestiferous moths. The method also should have applicability to organic growers by placing the traps outside of the fields or by using organic approved killing agents.

Publications

  • Landolt, P.J., C. Smithhisler, T. Adams, and R.S. Zack. 2003. An improved multi-component sex attractant for trapping male western yellowstriped armyworm, Spodoptera praefica (Grote) (Lepidoptea: Noctuidae). Agricultural and Forest Entomology 5: 1-7.


Progress 01/01/04 to 12/31/04

Outputs
Primarily through Leo Camelo, a PhD student, we continue to run field trials based on a number of baits and baiting regimes. Our results continue to indicate that we can influence the populations of moths in crops where their management is essential. Again, we are able to attract both males and females and to our "traps" which is a significant advance over chemical lures that attract only males. We continue to evaluate our results in terms of an overall reduction of pest status in the field with a concurrent reduction in damage.

Impacts
Although each cropping system is proving to be different, the results of our studies indicate that we can influence the reproductive potential of a pest population of moths with a concurrent reduction in pest numbers. We are also developing a better understanding of the bionomics of our study species, which is allowing us to better fine-tune our efforts. Specifically, we have developed good phenological information on reproductive development in the females and when one would need to trap in order to eliminate these females before they have had the opportunity to mate and or lay eggs.

Publications

  • Landolt, P. J., R. S. Zack, D. Green, and L. DeCamelo. 2004. Cabbage looper moths (Lepidoptera: Noctuidae) trapped with male pheromone. Florida Entomologist 87: 294-299.


Progress 01/01/03 to 12/31/03

Outputs
In a collaboration between and personnel from the USDA Lab in Wapato, WA, we have conducted and evaluated a first year of field research. We have tested several baits and are assessing their effectiveness at drawing moths (both males and females) to a source where they will obtain a lethal dose of an insecticide. Prelminary resullts indicate that we can attract moths to a bait and that we are able to reduce the number of adult moths in the field.

Impacts
We have found that we can influence the number of adult moths, both male and female, in the field through the use of bait stations. We will begin to conduct studies theif field season to see if a reduction in adult moths leads to a reduction in reproductive capabilities.

Publications

  • Landolt, P.J., C. Smithhisler, T. Adams and R.S. Zack. 2003. An improved multi-component sex attractant for trapping male western yellowstriped armyworm, Spodoptera praefica (Grote) (Lepidoptera: Noctuidae). Agricultural & Forest Entomology 5: 1-7.