Source: UNIV OF CALIFORNIA (VET-MED) submitted to
CHARACTERIZATION AND ANTIMICROBIAL RESISTANCE OF RUMINANT BACTERIAL PATHOGENS
Sponsoring Institution
Cooperating Schools of Veterinary Medicine
Project Status
ACTIVE
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
1033416
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Dec 5, 2024
Project End Date
Aug 7, 2029
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Project Director
LeCuyer, TE, .
Recipient Organization
UNIV OF CALIFORNIA (VET-MED)
(N/A)
DAVIS,CA 95616
Performing Department
Pathology, Microbiology & Immunology
Non Technical Summary
Antimicrobial resistance (AMR) is worldwide threat to public health. In order to combat AMR, we must better understand how resistant bacteria are transmitted in animal populations. The goal of this study is to better understand how AMR in cattle, sheep, and goats compares to AMR in dogs and cats. In addition, our goal is to understand how bacterial pathogens, including antimicrobial resistant bacteria, move between animals in herds and within production systems in California.
Animal Health Component
30%
Research Effort Categories
Basic
70%
Applied
30%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31140991100100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
4099 - Microorganisms, general/other;

Field Of Science
1100 - Bacteriology;
Goals / Objectives
The overall objective of this project is to gain a better understanding of infectious diseases of animals by studying the way that pathogens, particularly antimicrobial resistant pathogens, are transmitted within populations. Specific aims to achieve this objective are:1) Compare antimicrobial resistance genes in pathogens of ruminants compared to those in pathogens of companion animals in similar geographic regions2) Determine the molecular epidemiology of bacterial pathogenes of ruminants
Project Methods
Aim 1. Compare bacterial pathogens of ruminants to bacterial pathogens of companion animals in California.Aim 2. Compare bacterial pathogens of ruminants treated at the UC Davis VMTH with bacterial pathogens isolated from animal specimens submitted to CAHFS state diagnostic laboratories by comparing phenotypic and genotypic antimicrobial resistance as well as genetic background of bacterial pathogens.Methods for both aims:Isolates will be cryopreserved at -80C. DNA will be extracted using a commercial kit and bacteria will undergo short-read Illumina or long read Nanopore whole genome sequencing. Biofinformatic analyses will include identification of antimicrobial resistance genes and determination of sequence types. Similar organisms will undergo single-nucleotide polymorphism analysis. Phenotypic antimicrobial susceptiblity patterns will be determined by broth microdilution and will be compared.