Progress 02/15/15 to 02/14/19
Outputs
Target Audience:The target audiences included professionals, scientists, researchers and students (graduate and undergraduate) interested in the field of foods for health, plant biology, animal sciences, nutrition, inflammatory diseases and obesity. Changes/Problems:I have accepted a faculty position at Michigan State University (MSU) effective August 16, 2017. My last day at The Ohio State University will be August 15, 2016. Thus, I would like to request your consideration for transferring the remaining funding to my new institution, which will allow me to pursue the objectives of the grant. The co-PD, Dr. Erich Grotewold is also moving to MSU to become the Chair of Biochemistry and Molecular Biology. Our labs will be located in adjacent buildings connected by a bridge. The proximity of our two laboratories and the outstanding collaboration between researchers working in plants and health at MSU constitute a great environment to pursue the proposed studies and has been a great motivation to accept my new position. What opportunities for training and professional development has the project provided?Several training opportunities took place during this funded period. This project has contributed to the training of a PhD student, three postdocs, an undergraduate honor student from The Ohio State University We also hosted an undergraduate from University of Sao Paulo, Brazil, which was awarded a fellowship from her country and helped in some aspects of this project. In addition, the PD Dr. Doseff participated in the USDA grant training opportunity at Delaware State University (May 2017) and at the Symposium on Plant Biotechnology for Health and Sustainability at Michigan State University (October, 2015). How have the results been disseminated to communities of interest?Results have been communicated by several means, which included seven oral presentations by the PD, Dr. Doseff at Michigan State University, University of Pittsburgh, University of Florida, The Ohio State University and Experimental Biology. Several presentation by the co-PD, six poster presentations by the graduate student, two poster presentations by the postdoctoral trainees at the Heart and Lung Research Institute Research Day, The Klein Symposium in Nutrition, the Physiology Research day; the Wexner Medical Center Research day and two posters presentations by the undergraduate student. During this funded period we published three manuscripts, one review and one book chapter. What do you plan to do during the next reporting period to accomplish the goals?We will continue with the proposed experiments to study the effect of specific maize lines expressing different amount of flavones in the regulation to inflammation-induced obesity. During the next period we will evaluate the inflammatory markers, adipocytes and macrophages found in adipocyte tissues recently collected in dietary intervention experiments in which the flavone-rich maize was supplemented and provided in conjunction with the high fat diet. Additionally we will continue the proposed experiments to study the effects of whole-maize foods rich in flavones to treat inflammation-induced obesity. The results so far obtained showed the significant effect of flavones present in maize to reduce body weight and adiposity, demonstrating the relevance of our studies.
Impacts
What was accomplished under these goals?
The major goal of this project is to investigate the potential of maize-based flavone-rich diets in inflammationinduced obesity. The specific objectives for this proposal are: 1) Determine the bioavailability of flavones from whole-maize based flavone rich foods in vivo. 2) Evaluate the effects of maize-based flavone-rich diets in inflammation-induced obesity using animal models and NF-κB transgenic mice. During this period, the maize plants that differ in flavone content were grown and harvested. We conducted the analytical characterization of the kernels at different days after pollination and determined flavone content. The preliminary results of the analytical characterization, included on the submission of the grant, have been accepted for publication (Casas et al. Front. Plant Sci. 2014). As proposed we optimized the preparation of whole maize extracts using these maize lines and conducted the chemical characterization of flavone content in both the maize kernels and the food extracts. We compared the content of flavones in maize lines and in commercially supplements alleging to have apigenin, a main flavone found in the maize P-rr maize line. We found that only one of all the apigenin supplements tested contained the reported amount of apigenin, while all others had no flavones (Parihar et al. J. Med. Active Plants 2015). These results highlight the relevance to regulate supplements, which are currently FDA exempted. Additionally, these results highlight the relevance to use whole foods rich in flavones instead of supplements for the prevention and treatment of inflammatory inflammation. The maize extracts were used to evaluate the anti-inflammatory activity of flavones in whole foods. We found that flavones present in maize food extracts from P-rr line reduce inflammation as depicted by the reduction of the inflammatory cytokine TNFα in LPS-stimulated macrophages. Additionally, we conducted experiments to examine the ability of maize extracts to regulate adipocyte oil content and macrophage accumulation. We found that maize from P-rr reduced oil content in adipocytes. These preliminary studies suggested that flavone-rich maize extracts seem to delay adipocyte differentiation. Aligned to our goal of understanding the role of inflammation in obesity, during this period we collaborated with Dr. Stanford's team and evaluated the effects of macrophages in the lipid profile in obese mice sedentary or exposed to exercise. We showed that exercise reduces the number of macrophages in the adipose tissue and contributed to change the lipid profile. These studies have been recently published (May et al,Cell Reports, 2017). We next optimized the preparation of whole maize extracts to developed foods for mice with different flavone content We have conducted preliminary experiments using P-rr whole maize foods to evaluate the effect of the flavone-rich diet to modulate obesity. Our exiting results showed a significant reduction of body weight in mice consuming the whole-maize P-rr food, as compared with HFD. Additionally, we observed a significant reduction of liver weight and adiposity in brown, retroperitoneal, epididymal and inguinal tissues Organs from these mice have been collected and will be used to determine the effects of maize flavone-rich diets in macrophage accumulation and inflammatory cytokine levels in the different adipose tissues. These exiting results demonstrate the ability of whole maize foods to modulate obesity and support the relevance of pursuing the goals proposed in our grant. Products also included three publications, one book chapter, seven presentations by the PD Dr. Doseff, one at University of Florida, three at Michigan State University, one at The Ohio State University, one at the Experimental Biology meeting and one at the University of Pittsburgh. In addition, during this period of funding we presented four posters and 3 oral presentations by trainees.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
1. May, F.J., Baer, L.A., Lehnig, A.C., So, K., Chen,E.Y., Gao, F., Narain, N.R., Gushchina, L., Rose, A., Doseff,
A.I., Kiebish, M.A., Goodyear, L.J., and Stanford, K.I. (2017). Lipidomic adaptations in white and brown adipose
tissue in response to exercise demonstrate molecular species-specific remodeling. Cell Reports. 18: 15581572.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2015
Citation:
2. Parihar, A., Duarte, S. and Doseff, A.I. (2015). The saga of food supplements: safety and missing knowledge. J.
Med. Active Plants. 4: 16-19. recently accepted on Cell Reports
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Casas, M.I., Duarte, S.M., Doseff, A.I.#, and Grotewold, E#. (2014) Flavone-rich maize: An opportunity to
improve the nutritional value of an important commodity crop. Frontiers in Plant Sciences. 5: 1-11. #Shared
corresponding author.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Jiang, N., Doseff, A.I., and Grotewold, E. (2016). Flavones: From biosynthesis to health benefits. Plants (Special
issue). 5 (2). PMID: 27338492
- Type:
Book Chapters
Status:
Awaiting Publication
Year Published:
2017
Citation:
Parihar, A., and Doseff, A.I. (2017). Flavonoids in Brain health: Mechanisms and emerging roles of plant
nutraceuticals. In Pigments in Fruits and Vegetables: Genomics and Dietetics. Dr. (Ed.), Springer. In Press.
|
Progress 02/15/17 to 02/14/18
Outputs
Target Audience:The target audiences included professionals, scientists, researchers and students (graduate and undergraduate) interested in the field of foods for health, plant biology, animal sciences, nutrition, inflammatory diseases and obesity. Changes/Problems:I have accepted a faculty position at Michigan State University (MSU) effective August 16, 2017. My last day at The Ohio State University will be August 15, 2016. Thus, I would like to request your consideration for transferring the remaining funding to my new institution, which will allow me to pursue the objectives of the grant. The co-PD, Dr. Erich Grotewold is also moving to MSU to become the Chair of Biochemistry and Molecular Biology. Our labs will be located in adjacent buildings connected by a bridge. The proximity of our two laboratories and the outstanding collaboration between researchers working in plants and health at MSU constitute a great environment to pursue the proposed studies and has been a great motivation to accept my new position. What opportunities for training and professional development has the project provided?Several training opportunities took place during this funded period. This project has contributed to the training of a PhD student, three postdocs, an undergraduate honor student from The Ohio State University We also hosted an undergraduate from University of Sao Paulo, Brazil, which was awarded a fellowship from her country and helped in some aspects of this project. In addition, the PD Dr. Doseff participated in the USDA grant training opportunity at Delaware State University (May 2017) and at the Symposium on Plant Biotechnology for Health and Sustainability at Michigan State University (October, 2015). How have the results been disseminated to communities of interest?Results have been communicated by several means, which included seven oral presentations by the PD, Dr. Doseff at Michigan State University, University of Pittsburgh, University of Florida, The Ohio State University and Experimental Biology. Several presentation by the co-PD, six poster presentations by the graduate student, two poster presentations by the postdoctoral trainees at the Heart and Lung Research Institute Research Day, The Klein Symposium in Nutrition, the Physiology Research day; the Wexner Medical Center Research day and two posters presentations by the undergraduate student. During this funded period we published three manuscripts, one review and one book chapter. What do you plan to do during the next reporting period to accomplish the goals?We will continue with the proposed experiments to study the effect of specific maize lines expressing different amount of flavones in the regulation to inflammation-induced obesity. During the next period we will evaluate the inflammatory markers, adipocytes and macrophages found in adipocyte tissues recently collected in dietary intervention experiments in which the flavone-rich maize was supplemented and provided in conjunction with the high fat diet. Additionally we will continue the proposed experiments to study the effects of whole-maize foods rich in flavones to treat inflammation-induced obesity. The results so far obtained showed the significant effect of flavones present in maize to reduce body weight and adiposity, demonstrating the relevance of our studies.
Impacts
What was accomplished under these goals?
The major goal of this project is to investigate the potential of maize-based flavone-rich diets in inflammationinduced obesity. The specific objectives for this proposal are: 1) Determine the bioavailability of flavones from whole-maize based flavone rich foods in vivo. 2) Evaluate the effects of maize-based flavone-rich diets in inflammation-induced obesity using animal models and NF-κB transgenic mice. During this period, the maize plants that differ in flavone content were grown and harvested. We conducted the analytical characterization of the kernels at different days after pollination and determined flavone content. The preliminary results of the analytical characterization, included on the submission of the grant, have been accepted for publication (Casas et al. Front. Plant Sci. 2014). As proposed we optimized the preparation of whole maize extracts using these maize lines and conducted the chemical characterization of flavone content in both the maize kernels and the food extracts. We compared the content of flavones in maize lines and in commercially supplements alleging to have apigenin, a main flavone found in the maize P-rr maize line. We found that only one of all the apigenin supplements tested contained the reported amount of apigenin, while all others had no flavones (Parihar et al. J. Med. Active Plants 2015). These results highlight the relevance to regulate supplements, which are currently FDA exempted. Additionally, these results highlight the relevance to use whole foods rich in flavones instead of supplements for the prevention and treatment of inflammatory inflammation. The maize extracts were used to evaluate the anti-inflammatory activity of flavones in whole foods. We found that flavones present in maize food extracts from P-rr line reduce inflammation as depicted by the reduction of the inflammatory cytokine TNFα in LPS-stimulated macrophages. Additionally, we conducted experiments to examine the ability of maize extracts to regulate adipocyte oil content and macrophage accumulation. We found that maize from P-rr reduced oil content in adipocytes. These preliminary studies suggested that flavone-rich maize extracts seem to delay adipocyte differentiation. Aligned to our goal of understanding the role of inflammation in obesity, during this period we collaborated with Dr. Stanford's team and evaluated the effects of macrophages in the lipid profile in obese mice sedentary or exposed to exercise. We showed that exercise reduces the number of macrophages in the adipose tissue and contributed to change the lipid profile. These studies have been recently published (May et al,Cell Reports, 2017). We next optimized the preparation of whole maize extracts to developed foods for mice with different flavone content We have conducted preliminary experiments using P-rr whole maize foods to evaluate the effect of the flavone-rich diet to modulate obesity. Our exiting results showed a significant reduction of body weight in mice consuming the whole-maize P-rr food, as compared with HFD. Additionally, we observed a significant reduction of liver weight and adiposity in brown, retroperitoneal, epididymal and inguinal tissues Organs from these mice have been collected and will be used to determine the effects of maize flavone-rich diets in macrophage accumulation and inflammatory cytokine levels in the different adipose tissues. These exiting results demonstrate the ability of whole maize foods to modulate obesity and support the relevance of pursuing the goals proposed in our grant. Products also included three publications, one book chapter, seven presentations by the PD Dr. Doseff, one at University of Florida, three at Michigan State University, one at The Ohio State University, one at the Experimental Biology meeting and one at the University of Pittsburgh. In addition, during this period of funding we presented four posters and 3 oral presentations by trainees.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
1. May, F.J., Baer, L.A., Lehnig, A.C., So, K., Chen,E.Y., Gao, F., Narain, N.R., Gushchina, L., Rose, A., Doseff,
A.I., Kiebish, M.A., Goodyear, L.J., and Stanford, K.I. (2017). Lipidomic adaptations in white and brown adipose
tissue in response to exercise demonstrate molecular species-specific remodeling. Cell Reports. 18: 15581572.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2015
Citation:
2. Parihar, A., Duarte, S. and Doseff, A.I. (2015). The saga of food supplements: safety and missing knowledge. J.
Med. Active Plants. 4: 16-19. recently accepted on Cell Reports
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Casas, M.I., Duarte, S.M., Doseff, A.I.#, and Grotewold, E#. (2014) Flavone-rich maize: An opportunity to
improve the nutritional value of an important commodity crop. Frontiers in Plant Sciences. 5: 1-11. #Shared
corresponding author.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Jiang, N., Doseff, A.I., and Grotewold, E. (2016). Flavones: From biosynthesis to health benefits. Plants (Special
issue). 5 (2). PMID: 27338492
- Type:
Book Chapters
Status:
Awaiting Publication
Year Published:
2017
Citation:
1. Parihar, A., and Doseff, A.I. (2017). Flavonoids in Brain health: Mechanisms and emerging roles of plant
nutraceuticals. In Pigments in Fruits and Vegetables: Genomics and Dietetics. Dr. (Ed.), Springer. In Press.
|
Progress 02/15/16 to 02/14/17
Outputs
Target Audience:The target audiences included professionals, scientists, researchers and students (graduate and undergraduate) interested in the field of foods for health, nutrition, inflammatory diseases and obesity. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?From the perspective of education, this project contributes to the training of a PhD student, postdocs and an undergraduate honor student in the laboratory in areas pertinent to this project. How have the results been disseminated to communities of interest?Results have been communicated by several means, which included four oral presentations by A.I. Doseff at Michigan State University, University of Pittsburgh, The Ohio State University and Experimental Biology. Several presentation by the co-PD, three poster presentations by the graduate student Ms. S. Duarte at the Heart and Lung Research Institute Research Day, The Klein Symposium in Nutrition, and the Physiology Research day; and two poster presentations by Dr. Pereira at the Heart and Lung Research Institute Research Day and the Physiology Research day. What do you plan to do during the next reporting period to accomplish the goals?We will continue with the proposed experiments to study the effect of specific maize lines expressing different flavone contents in the mechanisms related to inflammation-induced obesity.
Impacts
What was accomplished under these goals?
Activities included growth of maize plants representing genotypes with diverse content of flavones. Development of the foods and assessment of their chemical composition. Identification of a comprehensive panel of different flavones with health related functions by LC-MS/MS. Animal experiments to establish bioavailability of the maize food diets. Animal experiments to determine the macrophage infiltration in adipose tissues. In addition, a paper has just been accepted for publication on Cell Reports (underlined names correspond to people that are part of this grant) Lipidomic Adaptations in White and Brown Adipose Tissue in Response to Exercise Demonstrates Molecular Species-Specific Remodeling. Francis J. May, Lisa A. Baer, Adam C. Lehnig, Kawai So, Emily Y. Chen, Fei Gao, Niven R. Narain, Liubov Gushchina, Aubrey Rose, Andrea I. Doseff, Michael A. Kiebish, Laurie J. Goodyear, and Kristin I. Stanford.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2017
Citation:
Lipidomic Adaptations in White and Brown Adipose Tissue in Response to Exercise Demonstrates Molecular Species-Specific Remodeling. Francis J. May, Lisa A. Baer, Adam C. Lehnig, Kawai So, Emily Y. Chen, Fei Gao, Niven R. Narain, Liubov Gushchina, Aubrey Rose, Andrea I. Doseff, Michael A. Kiebish, Laurie J. Goodyear, and Kristin I. Stanford.Cell Reports. In press
|
Progress 02/15/15 to 02/14/16
Outputs
Target Audience:The target audiences included researchers and students (graduate and undergraduate)with an interest in beneficial effects of plant diets.Efforts as part of this project included numerous lectures given by PI Doseff and a poster presentation given by Ms. Duarte. Dr. Doseff was also an invited speaker in the Plant Biotechnology Symposium, at the University of Michigan, a training initiative for trainees interested in Plant Applications funded by the NIH. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?From the perspective of education, this project contributes to the training of students and postdocs in the laboratory in areas pertinent to this project. How have the results been disseminated to communities of interest?Results have been communicated by several means, which included three oral presentations by A.I. Doseff at University of Florida, University of Michigan and at The Ohio State University and a poster presentation by S. Duarte at the Heart and Lung Research Institute Research Day. What do you plan to do during the next reporting period to accomplish the goals?As proposed in the orgiinal application, we will conduct tissue culture and animal experiments to study the effect of flavone-rich maize-based diets on inflammation-induced obesity.
Impacts
What was accomplished under these goals?
Activities included growth of maize plants with different genetic genotype that have distinct flavone content. Preparation and optimization of maize food extracts, chemical characterization of flavone content in food extracts and preparation of food for tissue culture and animal experiments. Animal experiments to establish bioavailability of the maize food diets. The outcomes and impact of this project can be evaluated at different levels: From the perspective of plants, our findings that different maize lines have distinct flavone content provide an ideal platform to reintroduce genetic traits that were selected against during domestication to improve food quality; the ability to use crops of great agronomical significance to ameliorate inflammatory diseases, including obesity. Results from this funding period also include the preparation of maize foods and some currently ongoing animal studies.
Publications
|
|