Progress 02/01/15 to 01/31/17
Outputs
Target Audience:Target audiences would include professionals, researchers and scientists in the area of food microbiology, food technology, biotechnology, microbiology, food safety as well as cconsumers, industrial professionals and the general public. Efforts include: laboratory instruction, development of innovative teaching methodologies; experiential learning opportunities. Changes/Problems:Request to Relinquish of Remaining Award Funds to New Institution was submitted on July 24, 2015. A final report for this purpose was requested on Aug. 2015. What opportunities for training and professional development has the project provided?Throughout the conduct of this project until its completion, it provides research opportunities for graduate student and professional staff. These training opportunities allowed the students to participate in exploring the science behind developing a novel detection method for significant foodborne pathogens. Through mentoring, training and independent laboratory activities, the student is able to learn the necessary techniques to carry out the objectives and develop meaningful protocols based on validated data and standards. The project was presented to international conference (Institute of Food Technologists - IFT Annual Conferences 2015). How have the results been disseminated to communities of interest?The results of this project have been presented in various meetings within and outside the university. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The project official award was received on Jan. 31, 2015 for the performance period from 02/ 01/2015 to 01/31/2017. Request to Relinquish of Remaining Award Funds to New Institution was submitted on July 24, 2015. A final report for this purpose was requested on Aug. 2015. We have made significant progress in objective 1 in developing a sufficient labeling tool. We developed on iron nanoparticle (IONP) particles by mixing FeCl3 and FeCl2 with addition of 25% ammonia. These IONP were coupled with flurophore using 10ul of fluorescein isothiocyanate (FITC) and then incubated at 400C for 1hr. Escherichia coli was used as a model to evaluate the established detection method. Different concentrations of IONP (100X, 75X, and 50X) and O.D600 values of E. coli cultures ranging from 0.1 to 0.6 were tested to establish an efficient labeling and detection method. Samples were divided into four groups including control groups that have only BHI broth with or without bacteria or IONP particles and a group with E. coli culture and IONP to which 1 ml of BHI broth, 0.5ml of the overnight culture and 6.25ul of flurophore-coupled IONP were added and incubated at 370C overnight. The labeling efficiency was evaluated based on bacterial fluorescence using confocal microscopy. Our results indicate that IONP can be successfully prepared and coupled with fluorophore. The labeling and detection of bacteria using IONP can be achieved by using 50X concentration of IONP and 0.1 O.D600 of bacterial culture. As the control group did not show any bacterial fluorescence it was evident that the bacteria took up the IONP that were coupled with flurophore and this labeling can be used for detection and also tracking of microorganisms. We are optimizing this technique and prepare for the next steps of the project. We have also had on-site project meetings with our collaborators at UC Davis Nuclear Magnetic Resonance Facility. We are planning on further training conducted at UC Davis and initiating preliminary testing in the coming Fall as soon as the project is successfully transferred to USDA ARS Western Regional Research Center (WRRC), Albany, California.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Tadepalli, S., D. Bridges, and V.C.H. Wu*. 2015. Development of fluorophore coupled iron oxide nanoparticles as a detection tool for microbial contamination. Institute of Food Technologist Annual Meeting. Chicago, IL.
|