Source: UNIVERSITY OF ARKANSAS submitted to
MOLECULAR MECHANISMS OF PLANT DISEASE RESISTANCE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0181632
Grant No.
(N/A)
Project No.
ARK01792
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jan 30, 1999
Project End Date
Sep 30, 2004
Grant Year
(N/A)
Project Director
Yang, Y.
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
PLANT PATHOLOGY
Non Technical Summary
Plant diseases result in significant losses of crops. Understanding the molecular mechanisms by which plant defend themselves is crucial for the development of disease resistant crops. Various genes associated with plant defense responses will be isolated and the molecular mechanisms of disease resistance will be studied.
Animal Health Component
20%
Research Effort Categories
Basic
80%
Applied
20%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21224101160100%
Goals / Objectives
1) Identify pathogen-induced transcription factors from tobacco and determine their role in plant disease resistance by transgenic analyses. 2) Utilize database mining, homology cloning, differential display, subtractive screening and other approaches to isolate rice genes involved in disease resistance. 3) Introduce useful genes into rice, manipulate plant defense signaling pathways and develop disease resistant transgenic rice.
Project Methods
Transgenic analysis will be conducted to determine the role of pathogen-induced Myb transcription factors in disease resistance. Various techniques such as PCR-based homology cloning, subtractive cloning, differential display will be used to isolate rice genes associate with disease resistance and susceptibility. The Agrobacterium-mediated transformation method will be used to introduce various resistance and defense genes into Arkansas rice cultivars and to develop transgenic plant with enhanced disease resistance.

Progress 01/30/99 to 09/30/04

Outputs
A combination of molecular, biochemical, genetic and genomic approaches has been used to elucidate the molecular mechanisms leading to disease resistance in rice. A number of significant progress have been made during the past six years, which include: 1) identification and sequence analysis of hundreds of defense-related rice genes; 2) generation and characterization of many transgenic rice lines defective in salicylic acid, jasmonic acid, ethylene or abscisic acid signal transduction; 3) molecular characterization and functional genomic analysis of stress-responsive mitogen-activated protein kinases and their role in disease resistance and abiotic stress tolerance; 4) molecular characterization and functional genomic analysis of pathogen-inducible myb transcriptional factors and their role in disease resistance; 5) identification and characterization of rice panicle blight pathogen and development of rapid and specific molecular diagnostic methods for detecting rice blast, rice sheath blight and rice bacterial blight pathogens.

Impacts
Rice is one of the most important crops in Arkansas and around the world. Furthermore, rice is an excellent model plant for cereal crops because of its small genome, extensive genetic mapping data, complete genomic sequences, relatively easy of transformation and synteny with other cereal genomes. The success of this study will not only enhance our understanding of host defense response in rice, but also help elucidate molecular mechanisms of disease resistance in other monocots and facilitate the development of novel strategies for disease control of economically important cereal crops.

Publications

  • Zhou, X. and Yang, Y. 2005. Differential expression of rice Nramp genes in response to pathogen infection, defense signal molecules and metal ions. Physiological and Molecular Plant Pathology (In press).
  • Lee, M.-W and Yang, Y. 2005. Agrobacterium-mediated transient expression assay in Arabidopsis. Methods in Molecular Biology: Arabidopsis Protocol (2nd edition), Eds. J. Salinas and J. J. Sanchez-Serrano, Humana Press (in press).
  • Yang, Y., Qi, M. and Mei, C. 2004. Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress. Plant Journal 40: 909-919.
  • Thara, V. K., Seilaniantz, A.R., Deng, Y., Dong, Y., Yang, Y., Tang, X., and Zhou, J.-M. 2004. Tobacco genes induced by the bacterial effector protein AvrPto. Mol. Plant-Microbe Interact. 17: 1139-1145.
  • Yang, Y., Qi, M., Lee, M.-W., and Xiong, L. 2003. Jasmonic acid- and salicylic acid-mediated defense signal transduction in rice. In Advances in Rice Genetics. pp429-431. Eds. G.S. Khush, D.S. Brar and B. Hardy. International Rice Research Institute.
  • Qi, M., and Yang, Y. 2002. Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphoimaging analyses. Phytopathology 92: 870-876.
  • Wang, S., Riggs, R.D., and Yang, Y. 2001. Grouping of populations of Heterodera trifolii by host preference and AFLP pattern. Nematology 3: 667-674.
  • Xiong, L., Qi, M., Lee, M. and Yang, Y. 2001. Identification of rice defense-related genes by subtractive cloning and differential screening. Mol. Plant-Microbe Interact. 14: 685-692.
  • Qi, L. Xiong, and Yang, Y. 2001. Effects of jasmonic acid and salicylic acid on basic PR-1 gene expression and induced resistance in rice. B.R.Wells Rice Research Studies pp 328-334, Arkansas Agricultural Experiment Station.
  • Yang, Y., Qi, M., Lee, M.-W. 2001. Host cell death and disease susceptibility. Phytopathology: S98.
  • Qi, M. and Yang, Y. 2001. Quantification of Magnaporthe grisea in infected rice leaves using real-time PCR. Phytopathology 91: S73.
  • Jia, Y., Correll, J. C., Lee, F. N., Eizenga, G.C., Yang, Y., Gealy, D.R., Valent, B., and Zhu, Q. 2001. Understanding molecular interaction mechanism of the Pita rice resistance gene and the rice blast pathogen. Phytopathology 91:S44.
  • Reyna, N., Xiong, L., Yang, Y. 2001. Characterization of a pathogen-induced rice cDNA homologous to the stress-responsive bacterial RelA/SpoT genes. The 10th International congress of Molecular Plant-Microbe Interactions, Madison, Wisconsin.
  • Yang, Y. and Qi, M. 2001. Anti-oxidative role of salicylic acid in rice plants. The 10th International congress of Molecular Plant-Microbe Interactions, Madison, Wisconsin.
  • Xiong, L., Qi, M. and Yang, Y. 2001. Involvement of a novel pathogen-activated rice MAP kinase in host defense response. The 10th International congress of Molecular Plant-Microbe Interactions, Madison, Wisconsin.
  • Lee, M.-W. and Yang, Y. 2001. Rice JAmyb gene encodes a positive regulator of cell death and disease resistance. The 10th International congress of Molecular Plant-Microbe Interactions, Madison, Wisconsin.
  • Xiong, L., Lee, M.-W., Qi, M. and Yang, Y. 2000. Isolation of defense-related rice genes by differentially screening a blast fungus-induced cDNA library. B. R. Wells Rice Research Studies pp38-44, Arkansas Agricultural Experiment Station.
  • Xiong, L., Qi, M. and Yang, Y. 2000. Identification of defense-related immediate early genes from rice by suppression subtractive hybridization. B. R. Wells Rice Research Studies pp45-52, Arkansas Agricultural Experiment Station.
  • Yang, Y., Qi, M., Lee, M., and Xiong, L. 2000. Jasmonic acid- and salicylic acid-mediated defense signal transduction in rice. Proceedings of the 4th International Rice Genetics Symposium, International Rice Research Institute, Philippines.
  • Lee, M.-W. and Yang, Y. 2000. A jasmonic acid-inducible rice myb gene associated with fungal infection and host cell death. Phytopathology 90: S45.
  • Lee, M.-W., Qi, M. and Yang, Y. 2001. A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. Mol. Plant-Microbe Interact. 14: 527-535.
  • Yang, Y. 2001. Systemic signaling. In Encyclopedia of Life Sciences. Nature Publishing Group.
  • Yang, Y., Li, R., and Qi, M. 2000. In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. Plant Journal 22: 543-551.
  • Bailey, T. and Yang, Y. 2004. A stress responsive MAP kinase mediates abscisic acid and ethylene signal interaction in rice plants. The 2nd International Symposium on Rice Functional Genomics, Tucson, AZ, Nov. 15-17, 2004.
  • Sayler, R.J., Cartwright, R.D. and Yang, Y. 2004. A rapid and reliable PCR method for detecting Burkholderia glumae, the causal agent of panicle blight on rice. Phytopathology. 94: S92.
  • Mei, C., Qi, M., Zhang, S., Sheng, G. and Yang, Y. 2004. Inducible overexpression of rice allene oxide synthase gene increases endogenous jasmonic acid level, PR gene expression and host resistance to fungal infection. Phytopathology 94: S70.
  • Zhou, X., Mei, C. and Yang, Y. 2004. Disease resistance and abiotic stress tolerance in rice is inversely regulated by an EIN2 homolog-mediated interplay between ethylene and abscisic acid signaling. Annual Meeting of American Society of Plant Biologists, Orlando, FL, July 24-29, 2004.
  • Reyna, N. and Yang, Y. 2004. Molecular and functional characterization of a jasmonic acid-inducible MAP kinase in rice. Annual Meeting of American Society of Plant Biologists, Orlando, FL, July 24-29, 2004.
  • Mei, C., Zhou, X. and Yang, Y. 2004. Rice COI1 gene mediates hormone signal interactions and negatively regulates cell elongation and plant height. Annual Meeting of American Society of Plant Biologists, Orlando, FL, July 24-29, 2004.
  • Yang, Y. 2004. Defense signal interaction in disease resistance and abiotic stress tolerance. The 15th International Congress of Plant Protection, Beijing, China, May 11-16, 2004.
  • Yang, Y., Mei, C., Zhou, X., Qi, M., Reyna, N.S., and Bailey, T.A. 2004. Transgenic rice mutants for functional genomic studies of defense signal pathways. Plant and Animal Genome XII, p903, San Diego, CA, Jan. 10-14, 2004.
  • Lee, M.-W. and Yang, Y. 2003. Overexpression of a pathogen-inducible myb gene increase disease resistance in transgenic rice. B.R.Wells Rice Research Studies pp65-72, Arkansas Agricultural Experiment Station.
  • Sayler, R. J., Cartwright, R.D. and Yang, Y. 2003. A rapid and reliable PCR method for detecting Burkholderial glumae, the causal agent of bacterial panicle blight. B.R.Wells Rice Research Studies pp211-216, Arkansas Agricultural Experiment Station.
  • Yang, Y. 2003. Defense signal mutants for functional genomics of the rice-pathogen interaction. The first International Symposium on Rice Functional Genomics, p103, Shanghai, China, Nov. 19-21, 2003.
  • Mei, C., Zhou, X., and Yang, Y. 2003. Generation of jasmonate-insensitive transgenic rice via dsRNAi-mediated suppression of the COI1 ortholog. Phytopathology 93: S60.
  • Zhou, X., and Yang, Y. 2003. Generation of ethylene-insensitive transgenic rice via dsRNAi-mediated suppression of the EIN2 ortholog. Phytopathology 93: S95.
  • Yang, Y., Qi, M., Xiong, L., Lee, M.-W., and Reyna, N. 2002. Genetic and molecular dissection of defense signaling pathways in rice. The 29th Rice Technical Working Group Meeting, Little Rock, Arkansas, February 24 - 27, 2002.
  • Li, R., Klessig, D.F., and Yang, Y. 2000. Overexpression of a salicylic acid-inducible myb gene in transgenic tobacco enhances the N gene-mediated resistance to TMV. Phytopathology 90: S46.
  • Yang, Y. and Qi, M. 2000. Potential role of salicylic acid in maintaining redox balance of rice plants. Phytopathology 90:S86.
  • Yang, Y. and Qi, M. 1999. Production of transgenic rice plants using Agrobacterium-mediated transformation. B.R.Wells Rice Research Studies pp46-54. Arkansas Agricultural Experiment Station.
  • Yang, Y. 1999. Genetic engineering of disease resistance in rice. Rice Field Day, Stuttgart, Arkansas.
  • Yang, Y. and Qi, M. 1999. Structure and regulation of the pathogen-inducible myb1 promoter from tobacco. Phytopathology 89:S87.
  • Lee, M. and Yang, Y. 1999. Molecular cloning of rice myb genes induced by Pyricularia grisea. Phytopathology 89:S45.
  • Yang, Y. and Qi, M. 1999. Differential expression of rice PR-1 and PR-10 genes induced by blast fungus, elicitor, and chemical treatments. Phytopathology 89:S62.
  • Yang, Y., Li, R., and Lee, M. 1999. Involvement of plant myb genes in disease resistance and susceptibility. The 9th International Congress of Molecular Plant-Microbe Interactions, Amsterdam, The Netherland.
  • Yang, Y., Qi, M. 1998. Induction of rice PR-1 homologues by blast fungus and chemical treatment. 6th International Symposium on Rice Molecular Biology, Shanghai, China.
  • Yang, Y., Qi, M., Klessig, D.F., Fay, L.D. 1998. Isolation and characterization of the pathogen-inducible myb1 promoter from tobacco. Phytopathology 88: S101.
  • Yang, Y. and Klessig, D.F. 1998. Alternation of myb gene expression in tobacco affects disease resistance and susceptibility. Phytopathology 88: S101.
  • Wang, S., Riggs, R.D., and Yang, Y. 1998. Amplified fragment length polymorphisms in race populations of soybean cyst nematode. Phytopathology 88: S95.
  • Yang, Y. 1998. Role of pathogen-inducible transcription factors in plant disease resistance. Xiang-Shan Science Conference on Molecular Mechanisms of Plant Disease Resistance s Transgenic Resistance, Beijing, China.


Progress 01/01/03 to 12/31/03

Outputs
A combination of molecular, biochemical, genetic and genomic approaches has been used to elucidate the molecular mechanisms leading to disease resistance in rice. A number of significant progress have been made during the past year, which include: 1) sequencing of additional 400 defense-related rice genes; 2) inducible overexpression of rice allene oxide synthase for enhanced disease tolerance; 3) functional genomic analysis of stress-responsive mitogen-activated protein kinases in rice and their role in biotic and abiotic stress tolerance; 4) functional genomic analysis of pathogen-inducible myb transcriptional factors and their role in disease resistance; 5) identification and characterization of rice panicle blight pathogen and development of a rapid and specific molecular diagnostic method for the detection and quantification of the bacterial pathogen.

Impacts
Rice is one of the most important crops in Arkansas and around the world. Furthermore, rice is an excellent model plant for cereal crops because of its small genome, extensive genetic mapping data, complete genomic sequences, relatively easy of transformation and synteny with other cereal genomes. The success of this study will not only enhance our understanding of host defense response in rice, but also help elucidate molecular mechanisms of disease resistance in other monocots and facilitate the development of novel strategies for disease control of economically important cereal crops.

Publications

  • Xiong, L. and Yang. Y. 2003. Disease resistance and abiotic stress tolerance are inversely modulated by an abscisic acid-inducible MAP kinase. Plant Cell 15: 745-759.
  • Reyna, N. S., and Yang, Y. 2003. Molecular characterization of a novel jasmonic acid-inducible MAP kinase in rice. Phytopathology 93: S73 (Abstract).
  • Imboden, L., Yang, Y., Zhou, X., and Korth, K. L. 2003. Characterization of insect herbivory-inducible genes in rice, Oryza sativa. Annual Meeting of American Society of Plant Biologists, Honolulu, Hawaii, July 25-30, 2003.


Progress 01/01/02 to 12/31/02

Outputs
A combination of molecular, biochemical, genetic and genomic approaches has been used to elucidate the signaling pathways and molecular mechanisms leading to disease resistance in rice. A number of significant progress have been made during the past year, which include: 1) generation of transgenic rice mutants with increased and decreased levels of jasmonic acid by inducible over expression or suppression of allene oxide synthase; 2) generation of transgenic rice mutants insensitive to jasmonic acid by suppression of rice Coi1 gene via double-stranded RNA interference; 3) generation of transgenic rice mutants with reduced levels of ethylene; 4) generation of ethylene-insensive transgenic mutants by suppression of rice EIN2 gene via double-stranded RNA interference; 5) functional characterization of an abscisic acid-inducible rice MAP kinase which was shown to inversely modulated disease resistance and abiotic stress in our study; 6) functional characterization of a jasmonic acid-inducible rice myb gene which was shown to positively regulate biosynthesis of flavonoid phytoalexins and enhance disease resistance; 7) isolation and collection of about 400 defense-related rice genes for microarray analysis.

Impacts
Rice is one of the most important crops in Arkansas and around the world. Furthermore, rice is an excellent model plant for cereal crops because of its small genome, extensive genetic mapping data, complete genomic sequences, relatively easy of transformation and synteny with other cereal genomes. The transgenic defense signal mutants generated in this study are essential to the understanding of defense signaling pathways and will be broadly distributed to rice research community to facilitate the studies of disease resistance mechanisms. The success of this study will not only enhance our understanding of host defense response in rice, but also help elucidate molecular mechanisms of disease resistance in other monocots and facilitate the development of novel strategies for disease control of economically important cereal crops.

Publications

  • Qi, M., and Yang, Y. 2002. Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphoimaging analyses. Phytopathology 92: 870-876.
  • Yang, Y., Qi, M., Xiong, L., Lee, M.-W., and Reyna, N. 2002. Genetic and molecular dissection of defense signaling pathways in rice. The 29th Rice Technical Working Group Meeting, Little Rock, Arkansas, February 24 - 27, 2002.


Progress 01/01/01 to 12/31/01

Outputs
We have employed a combination of molecular, biochemical, genetic and genomic approaches to elucidate the molecular mechanism of disease resistance in rice and made the following progress: 1) examined antioxidant enzyme activities in salicylic acid-deficient transgenic rice and confirmed the anti-oxidative role of salicylic acid for protecting rice plants from oxidative damage induced by aging, pathogen infection, drought, salt and cold stresses; 2) generated transgenic rice with altered jasmonate metabolism by inducible overexpression or suppression of allene oxide synthase; 2) further characterized the role of a jasmonic acid-inducible rice myb gene in disease resistance and susceptibility; 3) isolated and characterized a stress-inducible mitogen-activated protein kinase; 4) quantified the growth of the blast fungus in rice by the real-time PCR and RNA blot/phosphoimaging analyses. These studies will not only help elucidate disease resistance mechanisms in rice, but also facilitate the development of novel strategies for disease control in rice and other cereal crops.

Impacts
Rice is one of the most important crops in Arkansas and around the world. Development of novel strategies for disease control should significantly benefit rice growers by reducing disease-related yield losses and improving grain quality.

Publications

  • Xiong, L., Qi, M., Lee, M. and Yang, Y. 2001. Identification of rice defense-related genes by subtractive cloning and differential screening. Mol. Plant-Microbe Interact. 14: 685-692.
  • Lee, M.-W., Qi, M. and Yang, Y. 2001. A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. Mol. Plant-Microbe Interact. 14: 527-535.
  • Yang, Y. 2001. Systemic signaling. In Encyclopedia of Life Sciences. Nature Publishing Group.
  • Yang, Y., Qi, M., Lee, M.-W. 2001. Host cell death and disease susceptibility. Phytopathology: S98 (Abstract).
  • Qi, M. and Yang, Y. 2001. Quantification of Magnaporthe grisea in infected rice leaves using real-time PCR. Phytopathology 91: S73 (Abstract).
  • Jia, Y., Correll, J. C., Lee, F. N., Eizenga, G.C., Yang, Y., Gealy, D.R., Valent, B., and Zhu, Q. 2001. Understanding molecular interaction mechanism of the Pita rice resistance gene and the rice blast pathogen. Phytopathology 91:S44 (Abstract).


Progress 01/01/00 to 12/31/00

Outputs
We studied the molecular mechanism of disease resistance in rice and have made the following progress: 1) conducted successful transformation of "Drew", a commercial rice cultivar in Arkansas; 2) identified about 60 pathogen- and/or jasmonic acid-induced rice genes by subtractive cloning and differential screening; 3) further characterized a jasmonic acid-induced myb gene (JAmyb) associated with fungal infection and host cell death; 4) produced transgenic rice with inducible overexpression of JAmyb and demonstrated that JAmyb encodes a transcription regulator of host cell death; 5) further characterized salicylic acid-deficient transgenic rice and demonstrated that salicylic acid functions as a constitutive antioxidant against oxidative damage caused by aging and environmental stresses. Rice is an economically important crop in Arkansas and around the world. Elucidation of disease resistance mechanisms in rice should facilitate the development of novel strategies for disease control.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 01/01/99 to 12/31/99

Outputs
We studied the molecular mechanism of disease resistance in rice and have made the following progress: 1) constructed full-length and subtractive cDNA libraries from Arkansas rice cultivar "Drew"; 2) identified a dozen of pathogen-induced rice defense genes by subtractive cloning; 3)isolated and characterized a jasmonic acid-induced Myb transcription factor associated with fungal infection and host cell death; 4) produced salicylic acid-deficient transgenic rice by the Agrobacterium-mediated transformation; and 5) determined the antioxidant role of salicylic acid in rice. Rice is an economically important crop in Arkansas and around the world. Elucidation of disease resistance mechanisms in rice should facilitate the development of novel strategies for disease control.

Impacts
(N/A)

Publications

  • No publications reported this period