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↕ Project Title	↓ Accession Number	↕ Sponsoring Agency	↕ Project Start Date	↕ Project End Date	↕ Project Status	↕ Recipient City Name	↕ Non-Technical Summary	↕ Objectives
IMPROVEMENT AND CHARACTERIZATION OF CLOSTRIDIUM BEIJERINCKII BA101	0185853	National Institute of Food and Agriculture	10/01/2000	09/30/2006	COMPLETE	URBANA	Currently 2.58 billion pounds of butanol is produced in the United States. If this is produced from corn, this would create a market for 509 million bushel of corn annually. Development of such a large corn market would improve economic conditions for farmers. Butanol can be produced from corn by fermentation using Clostridium beijerinckii BA101. We intend to develop superior strains for efficient production of butanol from corn. This project would improve economic conditions of farmers. It is anticipated that newly developed strains would ferment corn efficiently.	1. Clostridium beijerinckii BA101 strain development for solvent production. 2. Use of AFLP to identify genetic markers associated with various derivatives of C. beijerinckii 8052. 3. Examination of Non-PTS based sugar transport in C. beijerinckii BA101 and 8052. 4. Physiological characterization of the newly constructed C. beijerinckii mutant and recombinant strains. 5. Downstream processing of butanol from fermentation broth.
PROTEASOMES IN THE ARCHAEA	0177264	State Agricultural Experiment Station	11/20/1997	03/31/2009	ACTIVE	GAINESVILLE	The Archaea, such as methanogens and hyperthermophiles, play a major role in the global carbon cycle and production of beneficial products due to their extreme metabolic diversity. However, very little is known about protein turnover in this class of organisms. The purpose of this study is to learn more about the role of energy-dependent proteolysis as a regulatory process in the Archaea.	The objectives of this project are to investigate the structure and function of the proteasome (a) large-molecular-weight proteinase) from the acetotrophic methanogen Methanosarcina thermophila. The results are expected to: (i) advance the field of acetotrophic methanogenesis which accounts for over 60% of the biologically produced methane (a green-house gas), (ii) expand the fundamental knonwledge of the evolution, mechanism, and function of proteasomes in all of nature; (iii) provide a broader underestanding of the biochemistry, genetics, and physiology of M. thermophila and the methanogenic Archaea; and (iv) help to further define the evolutionary relationshps between the Archaea and Eucarya domains.

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