| Algae for conversion of manure nutrients to animal feed: Evaluation of advanced nutritional value, toxicity, and zoonotic pathogens | 1000956 | Murinda, Shelton | 09/01/2013 | 08/31/2017 | COMPLETE | Pomona | Algae, Animal Feed, Bacteria, Manure, Nutritional Value, Pathogens, Toxic Cyanobacteria | Rationale The need to control manure-derived nutrient pollution is straining the confined animal production industry. California is the top milk producing state and has some of the strictest nutrient regulations. But in the San Joaquin Valley, many dairies do not have affordable access to more land for manure application. A highly productive crop is needed that will convert manure nitrogen (N) and phosphate (P) into feed but in smaller land areas than crops such as corn. Algae are a candidate feed with annual yields typically 7-13 times greater than soy or corn. Beyond 40-50% protein, algae also contain fatty acids, amino acids, pigments, and vitamins that are valuable in animal feeds, especially for adding value to milk. Advances in molecular biology allow us to gather needed information on the risks and benefits of algae-based animal feeds. Overall goal Benefit animal agriculture and the environment by introducing microalgae as a fast-growing livestock feed crop. Aim 1 Cultivate algae in dairy freestall barn flush water, treating this wastewater, while producing algae feedstock at a high annual rate, at least 10-times greater than corn. Algae will be cultivated in 30-cm deep raceway ponds at the 300-head Cal Poly campus dairy farm where extensive manure management research already occurs under USDA and USEPA sponsorship. Aim 2 Produce algae with favorable nutritional characteristics (high digestibility, valuable fatty and amino acid profiles, balanced protein and carbohydrate concentration, etc.) by adjusting the treated-water recycling into the ponds to optimize the N concentration in the growth medium. Aim 3 Test pathogen survival in algae feeds prepared by pasteurization and/or drying and heating. A trend in municipal wastewater treatment is pasteurization of treated effluent using waste heat from natural gas electrical generator. Large dairies with digesters will have waste heat available for pasteurization and drying. High-protein algae will be pelletized with high carbohydrate feeds to create a balanced feed. The heat of pelletization also contributes to pasteurization. Cal Poly has a research feed mill for producing such blended feeds. Aim 4 Monitor contamination by cyanobacteria and any cyanobacterial toxins. Approach Removal of N, P, and other constituents will be optimized in influent and effluent of identical ponds. Algal biomass (harvested by bioflocculation+settling) will be analyzed for N, P, protein, carbohydrates, and profiles of fatty and amino acids. Pathogen and algal communities extant in raw and feed-processed algal biomass will be analyzed using metagenomics and pyrosequencing. Potential toxicity of algal biomass will be studied using toxicity evaluation of cell-free extracts on cultured mammalian cells. A TC 20 Cell counter (BioRad Laboratories) will be used to monitor toxicity events on treated cells using trypan blue staining. Cytotoxic positive samples will be tested for both presence and concentration of known cyanobacterial toxins. The researchers have decades' experience in algae production, wastewater treatment, and food safety. Expected outcomes Starting with dairy, the project will lead the way towards an algae feed industry based on advanced nutritional features to enhance agricultural products (e.g., milk protein, poultry pigment) while assisting farmers to meet manure management challenges. We will address topics rarely covered in the algae field: potential toxicity and zoonotic pathogens. Our approach is unique in that it integrates and addresses a triad of issues, namely, food safety issues along with algae production techniques and waste management. | Project Goals 1. Generate experimental field data and calibrate optimization models. For treatment, expected removals are 85-95% biochemical oxygen demand and soluble Nitrogen (N) and 40-80% solublePhosphate (P) removal, depending on culturing technique and season. 2. Maximize the nutritional value of produced algae for animal feed. The cultures will be optimized to produce biomass at a high rate while also having the highest value composition for feed (in terms of lipids, digestibility, essential fatty and amino acid profiles, including balanced protein and carbohydrate concentrations). 3. Optimize pathogen inactivation methods. Pathogens will die-off in the ponds and during disinfection processing of the harvested biomass. Inactivation rates for representative pathogen indicators will be determined under various algae cultivation conditions and during trials with several biomass disinfection techniques. The optimal combination of pond conditions (e.g., high pH) and biomass processing (e.g., pasteurization) will be determined to achieve needed log inactivation of pathogens, which is typically 1- >4 log10 reduction (Sobsey et al., Available Online). 4. Quantify and control any cyanobacterial toxins. qPCR assays described by Al-Tarineh et al. (2012 a and b) will be used and optimized to reliably determine the copy number of cyanotoxin biosynthesis genes, as well as an internal cyanobacteria 16S rDNA control, in a single reaction. The latter detects for presence of cyanobacteria. If toxins are detected, measures will be taken to control invasion of the ponds by cyanotoxin-producing cyanobacteria strains. Overall Goal Benefit agriculture and the environment by introducing microalgae, a fast-growing livestock feed crop. |
| Prediction and Control of the Performance of Anaerobic Digestion of Animal Manure through Metagenomics for Renewable Energy and a Sustainable Environment | 1000723 | Zheng, Guolu | 09/01/2013 | 08/31/2017 | COMPLETE | JEFFERSON CITY | Anaerobic digestion; Animal manure; Computational modeling; Antibiotic resistance genes; Pathogens | Improper collection and disposal of untreated animal waste can lead to serious pollution problems, such as pathogen contamination, spread of antibiotic resistance, and nutrient overflow, which pose risks to the environment and to public health. Unfortunately, the current animal waste treatment systems and practices are often inadequate. Anaerobic digestion technologies are superior both environmentally and financially when compared with traditional waste management systems, such as manure storages and lagoons. Anaerobic digestion is a biological process by which organic material, such as animal wastes, are decomposed in the absence of oxygen, producing stabilized sludge of agricultural value as well as methane (bio-gas), a renewable energy source. However, studies indicate that antibiotic resistance can survive anaerobic digestion. In addition, problems such as low methane yield and process instability are often encountered in anaerobic digestion. This project is to use computer to analysesthe correlation between theout puts of anaerobic digestion and themicrobial population, rather than on a few microorganisms (current methods), in the waste. Based on the computational relationship, we expect tomaximize the yield of methane (bio-gas), increase the process stabilization of anaerobic digestion, and the mitigation of pathogens and antibiotic resistance genes. | The goal of this proof-of-principle project is to eliminate/reduce the spread of the pathogens and antibiotic resistant genes associated with animal waste while maximizing the use of animal waste as a source of renewable energy and fertilizer. The specific objectives of this project are the following: Objective 1: Maximize the yield of methane, the stability of the anaerobic digestion, and the mitigation of pathogens and antibiotic resistance genes. Objective 2: Identify and use key microbial indicators for monitoring and control of the performance of anaerobic digestion to prevent its failure. |
| Attaining High Quality Soft White Winter Wheat through Optimal Management of Nitrogen, Residue and Soil Microbes | 0435472 | REARDON C L | 09/06/2018 | 09/05/2023 | COMPLETE | PENDLETON | INLAND, PACIFIC, NORTHWEST, DRYLAND, NITROGEN, REPLACEMENT, PRECISION, NITROGEN, MANAGEMENT, NEAR, INFRARED, SPECTROSCOPY, GRAIN, PROTEIN, CONCENTRATION, GRAIN, QUALITY, MICROBIAL, COMMUNITIES, BACTERIA, FUNGI, NUTRIENT, CYCLING, DROUGHT, STRESS, WATER, AVAILABILITY | Not applicable | Obj. 1: Extend the N replacement approach to soft white winter wheat for guiding precision management of fertilizer N and crop residue to optimize soil microbial processes and maximize the biological potential of soil. 1A: Evaluate grain protein concentration and yield response to N under varying levels of water to define the critical protein level and fertilizer N equivalent to a unit change in protein for popular cultivars of soft white winter wheat. 1B: Determine whether uniformity of protein levels in the crop can be achieved with the precision N replacement approach. 1C: Adapt instruments and algorithms to support on-farm implementation of the N replacement approach to precision fertilizer management in dryland wheat production systems. 1D: Evaluate the effects of residue management (standing, distributed on the soil surface, or removed) on the plant-available N, precipitation capture efficiency, crop productivity, weed density, and microbial activity during the 13 months of fallow. Obj. 2: Identify whether soil microbial communities adapted to dry environments benefit plant fitness under water limited conditions. 2A: Identify the composition of microbial consortia naturally adapted to low water availability. 2B: Determine whether cultivar selection and N management can be manipulated to shift the structure and function of microbial communities to benefit plants under water stress. Obj. 3. Develop resilient cropping systems and strategies that increase resilience, improve economic returns, and enhance ecosystem services; assess their economic and environmental performance of various cropping systems in concert with their supporting components; and develop decision support systems for optimizing agronomic production in these cropping systems. 3A: Compare economic returns from the variable N replacement approach based on previous seasonâ¿¿s site-specific SWW crop yield data and conventional uniform N placement based on field bulk soil sampling and laboratory testing. 3B: Increase dryland farming resilience by developing cropping systems more intensive and diverse than the conventional winter wheat-fallow system. 3C: Investigate the yields and economic returns of alternative crops following winter wheat and winter wheat following cover crops across low and intermediate precipitation zones using current and future climate scenarios. Obj. 4. Increase the sustainability resilience and tolerance of the dryland crop production system to biotic and abiotic stressors through improved understanding of developmental, environmental, and management factors that limit plant health and growth, including but not limited to stress tolerance, water use efficiency, and disease resistance. 4A: Evaluate stress indicators and yield components of wheat in alternative cropping systems compared to wheat-fallow with relation to soil water availability, disease incidence, and rotational crop morphology. 4B: Investigate crop response to water deficit, high temperature, and/or nitrogen availability. |
| Improvement of Soil Management Practices and Manure Treatment/Handling Systems of the Southern Coastal Plain | 0431207 | SZOGI A A | 07/27/2016 | 07/05/2021 | ACTIVE | FLORENCE | ANIMAL, AMMONIA, NITROGEN, PLANTS, PYROLYSIS, FERTILIZER, WATER, COVER, CROP, REDUCED, TILLAGE, NITRIFICATION, TREATMENT, ANAMMOX, EMISSIONS, SOIL, MANAGEMENT, DISCARDED, SOILS, SUSTAINABLE, PRODUCTION, PHOSPHORUS, REMOVAL, WASTE, SOLIDS, CARBON, GENES, GAS, PAHTOGEN, MANURE, QUALITY, RESIDUE, BIOCHAR, AMENDMENT, NITROUS, OXIDE | Not applicable | 1. Develop and test improved tillage and biomass management practices to enhance soil health and long-term agricultural productivity in the Southeastern Coastal Plain. 2. Develop manure treatment and handling systems that improve soil health and water quality while minimizing the emissions of greenhouse gases, odors and ammonia and the transport of phosphorus and pathogens. Subobjective 2a. Develop improved treatment systems and methods for ammonia and phosphorus recovery from liquid and solid wastes using gas-permeable membrane technology. Subobjective 2b. Develop improved biological treatment systems for liquid effluents and soils based on deammonification reaction using ARS patented bacterial anammox and high performance nitrifying sludge cultures. Subobjective 2c. Improve the ARS patented â¿¿Quick Washâ¿? process for phosphorus recovery. Subobjective 2d. Assess treatment methods for their ability to reduce or eliminate pathogens and cell-free, microbially-derived DNA from agricultural waste streams. Subobjective 2e. Improved manure treatment and handling systems, and management strategies for minimizing emissions. Subobjective 2f. Assess the impact of manure treatment and handling systems on agricultural ecosystem services for soil, water, and air quality conservation and protection. 3. Develop beneficial uses of agricultural, industrial, and municipal byproducts, including manure. Subobjective 3a. Evaluate application of designer biochars to soils to increase crop yields while improving soil health, increasing carbon sequestration, and reducing greenhouse gas emissions. Subobjective 3b. Develop methods and guidelines to remediate mine soils using designer biochars. Subobjective 3c. Evaluate the agronomic value of byproducts produced from emerging manure and municipal waste treatment technologies. |
| Enable New Marketable, Value-added Coproducts to Improve Biorefining Profitability | 0427684 | MOREAU R A | 09/08/2014 | 09/07/2019 | ACTIVE | WYNDMOOR | COPRODUCTS, BIOFUELS, ETHANOL, SORGHUM, BIODIESEL, CELLULOSE, HEMICELLULOSE, BRAN, GUMS | Not applicable | 1. Develop processes to fractionate sorghum and corn/sorghum oils into new commercially-viable coproducts. 2. Develop processes to fractionate grain-derived brans into new commercially-viable coproducts. 2a: Develop processes to fractionate grain-derived brans into new commercially-viable coproducts such as lipid-based coproducts and for other industrial uses such as extrusion or producing energy or fuel. 2b: Develop commercially-viable, value-added carbohydrate based co-products from sorghum brans and the brans derived from other grains during their biorefinery process. 3. Develop processes to fractionate biorefinery-derived celluloses and hemicelluloses into new commercially-viable coproducts. 3a: Develop commercially-viable, value-added hemicellulose based co-products from sorghum biomass, sorghum bagasse and other agricultural based biomasses produced during their biorefining. 3b: Develop commercially-viable, value-added cellulose based co-products from sorghum biomass, sorghum bagasse and other agricultural based biomasses produced during their biorefining. 4. Develop technologies that enhance biodiesel quality so as to enable greater market supply and demand for biodiesel fuels and >B5 blends in particular. 4a: Improve the low temperature operability of biodiesel by chemical modification of the branched-chain fatty acids. 4b: Develop technologies that significantly reduce quality-related limitations to market growth of biodiesel produced from trap and float greases. 4c: Further develop direct (in situ) biodiesel production so as to enable its commercial deployment. 5. Develop technologies that enable the commercial production of new products and coproducts at lipid-based biorefineries. 5a: Enable the commercial production of alkyl-branched from agricultural products and food-wastes. 5b: Enable the commercial production of aryl-branched fatty acids produced from a combination of lipids and natural antimicrobials possessing phenol functionalities. |
| Technologies for Improving Industrial Biorefineries that Produce Marketable Biobased Products | 0427427 | ORTS W J | 10/01/2014 | 09/30/2019 | COMPLETE | ALBANY | BIOPRODUCTS, BIOENERGY, SORGHUM, BIOMASS, POLYHYDROXYALKANOATES, POLYSACCHARIDES, BIOMASS, ENZYMES, FIBERS, COMBINATORIAL, CHEMISTRY, DIRECTED, EVOLUTION, NANOTECHNOLOGY, NANO-ASSEMBLIES, CELLULOSE, PECTIN, DIACIDS, POLYMERS, POLY(HYDROXYBUTYRATE), PHA, BIOFUELS, CITRUS, ALMONDS, EXTRACTION, RENEWABLE, FERMENTATION, BIOREFINERY, FOOD, WASTE, ENZYMES | Not applicable | This project provides technological solutions to the biofuels industry to help the U.S. meet its Congressionally mandated goal of doubling advanced biofuels production within the next decade. The overall goal is to develop optimal strategies for converting agricultural biomass to biofuels and to create value-added products (bioproducts) that improve the economics of biorefining processes. Specific emphasis is to develop strategies for biorefineries located in the Western United States by using regionally-specific feedstocks and crops, including sorghum, almond byproducts, citrus juicing wastes, pomace, municipal solid wastes (MSW), and food processing wastes. These feedstocks will be converted into biofuels, bioenergy and fine chemicals. Objective 1: Develop commercially-viable technologies for converting agriculturally-derived biomass, crop residues, biogas, and underutilized waste streams into marketable chemicals. Research on converting biogas will involve significant collaboration with one or more industrial partners. Sub-objective 1A: Provide data and process models for integrated biorefineries that utilize sorghum and available solid waste to produce ethanol, biogas and commercially-viable coproducts. Sub-objective 1B. Convert biogas from biorefining processes into polyhydroxyalkanoate plastics. Sub-objective 1C: Apply the latest tools in immobilized enzymes, nano-assemblies, to convert biomass to fermentable sugars, formaldehyde, and other fine chemicals. Objective 2: Develop commercially-viable fractionation, separation, de-construction, recovery and conversion technologies that enable the production of marketable products and co-products from the byproducts of large-scale food production and processing. Sub-objective 2A: Add value to almond byproducts. Sub-objective 2B: Apply bioenegineering of bacteria and yeast to produce diacids, ascorbic acid and other value-added products from pectin-rich citrus peel waste. Sub-objective 2C: Convert biomass into commercially-viable designer oligosaccharides using combinatorial enzyme technology. |
| Integration of Site-Specific Crop Production Practices and Industrial and Animal Agricultural Byproducts to Improve Agricultural Competitiveness and Sustainability | 0425032 | JENKINS J N | 10/01/2013 | 09/30/2018 | ACTIVE | MISSISSIPPI STATE | PRECISION, FARMING, GEOGRAPHIC, INFORMATION, SYSTEM, (GIS), REMOTE, SENSING, (RS), WATER, SWINE, ANIMAL, WASTE, AMMONIA, SOIL, NUTRIENTS, PATHOGEN, NITROGEN, LITTER, LEACHING, CROPS, RUNOFF, BACTERIA, BROILER | Not applicable | Obj 1. Develop ecological and sustainable site-specific agriculture systems, for cotton, corn, wheat, and soybean rotations. 1: Geographical coordinates constitutes necessary and sufficient cornerstone required to define, develop and implement ecological/sustainable agricultural systems. 2: Develop methods of variable-rate manure application based on soil organic matter (SOM), apparent electrical conductivity, elevation, or crop yield maps. 3: Relate SOM, electrical conductivity, and elevation. Obj 2. Develop sustainable and scalable practices for site-specific integration of animal agriculture byproducts to improve food, feed, fiber, and feedstock production systems. 1: Quantify effects of management on sustainability for sweet potato. 2: Balance soil phosphorus (P)/micro¿nutrients using broiler litter/flue gas desulfurization (FGD) gypsum. 3: Effects of site-specific broiler litter applications. 4: Manure application/crop management practices in southern U.S. 5: Compare banded/broadcast litter applications in corn. 6: Develop reflectance algorithms for potassium in wheat. 7: Determine swine mortality compost value in small farm vegetable production. Obj 3. Analyze the economics of production practices for site-specific integration of animal agriculture byproducts to identify practices that are economically sustainable, scalable, and that increase competitiveness and profitability of production systems. 1: Evaluate economics of on-farm resource utilization in the south. Obj 4. Determine the environmental effects in soil, water, and air from site-specific integration of animal agricultural and industrial byproducts into production practices to estimate risks and benefits from byproduct nutrients, microbes, and management practices. 1: Quantitatively determine bioaerosol transport. 2: Role of P and nitrogen (N) immobilizing agents in corn production. 3: Assess impact of management on water sources. 4: Impact of FGD gypsum/rainfall on mobilization of organic carbon/veterinary pharmaceutical compounds in runoff/leached water. 5: Assess soil microbial ecology, antibiotic resistance, and pathogen changes using manure and industrial byproducts in crop production systems. 6: Develop nutrient management practices for sustainable crop production. 7: Develop nutrient management practices for reclaimed coal mine soils. 8: Determine effects of poultry litter/swine lagoon effluent in swine mortality composts. 9: Determine survival of fecal bacterial pathogens on contaminated plant tissue. 10: Identify agricultural/industrial byproducts that modify the breakdown of organic matter. Obj 5. Integrate research data into regional and national databases and statistical models to improve competitiveness and sustainability of farming practices. 1: Develop broiler house emission models. 2: Apply quantitative microbial risk assessment models to animal agriculture/anthropogenic activities. Obj 6. Develop statistical approaches to integrate and analyze large and diverse spatial and temporal geo-referenced data sets derived from crop production systems that include ecological and natural resource based inputs. 1: Develop novel methods of imaging processing. |
| CONTROL OF HUMAN PATHOGENS ASSOCIATED WITH ACIDIFIED PRODUCE FOODS | 0420825 | BREIDT F | 12/02/2010 | 10/27/2015 | COMPLETE | RALEIGH | ESCHERICHIA, COLI, O157:H7, SALMONELLA, ACID, FOOD, CUCUMBER, ORGANIC, ACID, CUCUMIS, SATIVUS, BENZOIC, ACID, ACETIC, ACID, MALIC, ACID, ANAEROBIC, ACID, RESISTANCE, LISTERIA, MONOCYTOGENES, PICKLED, VEGETABLE, ACIDIFIED, FOOD, PEPPER, CAPSICUM, ANNUM, PRESERVATIVE, SORBIC, ACID, LACTIC, ACID, OXYGEN, AEROBIC, ACID-TOLERANT, PATHOGEN | Not applicable | 1. To define conditions to assure a 5 log reduction of acid tolerant pathogens in refrigerated or bulk stored acidified vegetables. 2. To determine how the metabolism of Escherichia coli O157:H7 (internal pH, membrane potential, ion concentrations, and cell metabolites) are affected as cells are exposed to organic acid and salt conditions typical of acidified foods. 3. To determine the survival of E. coli O157:H7 in commercial fermentation brines, with and without competing microflora, and under a variety of extrinsic and intrinsic conditions. |
| Efficient Management and Use of Animal Manure to Protect Human Health and Environmental Quality | 0420394 | SISTANI K R | 10/01/2010 | 09/30/2015 | COMPLETE | BOWLING GREEN | ANIMAL, MANURE, ODOR, NUTRIENT, BYPRODUCT, ATMOSPHERIC, EMISSIONS, KARST, TOPOGRAPHY, PATHOGEN, TREATMENT, TECHNOLOGY, MICROORGANISMS | Not applicable | The overall goal of the research project which is formulated as a real partnership between ARS and Western Kentucky University (WKU) is to conduct cost effective and problem solving research associated with animal waste management. The research will evaluate management practices and treatment strategies that protect water quality, reduce atmospheric emissions, and control pathogens at the animal production facilities, manure storage areas, and field application sites, particularly for the karst topography. This Project Plan is a unique situation in the sense that non-ARS scientists from WKU are included on an in-house project to conduct research under the NP 214. The objectives and related specific sub-objectives for the next 5 years are organized according to the Components (Nutrient, Emission, Pathogen, and Byproduct) of the NP 214, which mostly apply to this project as follows: 1) develop improved best management practices, application technologies, and decision support systems for poultry and livestock manure used in crop production; 2) develop methods to identify and quantify emissions, from poultry, dairy and swine rearing operations and manure applied lands; 3) reduce ammonia, odors, microorganisms and particulate emissions from dairy, swine and poultry operations through the use of treatment systems (e.g. biofilters and scrubbers) and innovative management practices; 4) perform runoff and leaching experiments on a variety of soils amended with dairy, swine, or poultry manures infected with Campylobacter jejuni (C. jejuni), Salmonella sp. or Mycobacterium avium subsp. paratuberculosis (MAP) and compare observed transport with that observed for common indicator organisms such as E. coli, enterococci, and Bacteriodes; and 5) use molecular-based methodologies to quantify the occurrence of pathogens and evaluate new methods to inhibit their survival and transport in soil, water, and waste treatment systems. |
| Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization | 0420348 | SZOGI A A | 10/01/2010 | 09/30/2015 | COMPLETE | FLORENCE | ANIMAL, WATER, PHOSPHORUS, TRACE, AMMONIA, DENITRIFICATION, REMOVAL, REDOX, OXYGEN, WETLAND, WASTE, QUALITY, NITROGEN, NITRIFICATION, SOLIDS, POTENTIAL, PLANTS, TREATMENT, CARBON, BIOCHAR, PYROLYSIS, ANAMMOX, GENES, AMENDMENT, FERTILIZER, EMISSIONS, GAS, NITROUS, OXIDE | Not applicable | 1. Develop improved treatment technologies to better manage manure from swine, poultry and dairy operations to reduce releases to the environment of odors, pathogens, ammonia, and greenhouse gases as well as to maximize nutrient recovery. 2. Develop renewable energy via thermochemical technologies and practices for improved conversion of manure into heat, power, biofuels, and biochars. 3. Develop guidelines to minimize nitrous oxide emissions from poultry and swine manure-impacted riparian buffers and treatment wetlands. 4. Develop beneficial uses of manure treatment technology byproducts. |
| BIOLOGICAL TREATMENT OF MANURE AND ORGANIC RESIDUALS TO CAPTURE NUTRIENTS AND TRANSFORM CONTAMINANTS | 0420063 | MULBRY III W W | 04/03/2010 | 04/02/2015 | COMPLETE | BELTSVILLE | SWINE, WASTE, SOIL, POULTRY, MANAGEMENT, DAIRY, EMMISION, MANURE, TREATMENT, ENVIRONMENTAL, BYPRODUCTS, FATE, ORGANIC, BIOENERGY, COMPOST, RESIDUE, DESTRUCTION, NUTRIENTS, APPLICATIONS, ANAEROBIC, DIGESTION, ALGAL, METHANE, AMMONIA, ANTIBIOTIC | Not applicable | Development and evaluation of manure treatment systems. Specific objectives: (1) Develop treatment technologies and management practices to reduce the concentrations of pharmaceutically active compounds (antibiotics and natural hormones) in manures, litters, and biosolids utilized in agricultural settings; (2) Develop management practices and technologies to minimize greenhouse gas (GHG) emissions from manure and litter storage and from composting operations by manipulating the biological, chemical, and physical processes influencing production and release of ammonia and greenhouse gases during composting; (3) Develop technology and management practices that improve the economics and treatment efficiency of anaerobic digestion of animal manures and other organic feedstocks (e.g. food wastes, crops/residues) for waste treatment and energy production. |
| Management of Manure Nutrients, Environmental Contaminants, and Energy From Cattle and Swine Production Facilities | 0420053 | WOODBURY B L | 10/01/2010 | 09/30/2015 | COMPLETE | CLAY CENTER | FEEDLOT, SURFACING, MATERIAL, BEEF, MONOSLOPE, FACILITIES, ANAEROBIC, DIGESTION, ENERGY, RECOVERY, COAL-ASH, WDGS, GREENHOUSE, GASES, AIR, QUALITY, PATHOGENS | Not applicable | Obj.1: Develop precision techniques or other methods for the characterization and harvesting of feedlot manure packs in order to maximize nutrient and energy value and minimize environmental risk. Obj.2: Determine the fate and transport of antibiotics (e.g., monensin and tetracyclines) and pathogens (e.g., E.coli O157:H7 and Salmonella and Campylobacter) in beef cattle and swine facilities. Obj.3: Quantify and characterize air emissions from beef cattle and swine facilities to evaluate and improve management practices. Obj.4: Determine the risk and benefits of using coal-ash and other industrial byproducts as a component of surfacing material for feedlot pens. |
| METABOLIC VARIABLES AFFECTING THE EFFICACY, SAFETY, AND FATE OF AGRICULTURAL CHEMICALS | 0410345 | SMITH D J | 02/03/2006 | 02/02/2011 | COMPLETE | FARGO | RESIDUE, CHEMICAL, FOOD, ANIMAL, DETECTION, METABOLISM, PATHOGEN, SOIL, MANURE, COMPOST, WATER | Not applicable | Objective 1: Determine metabolic variables (rates of absorption, tissue and microbial biotransformation, excretion) that positively or negatively influence the practical use of novel pre-harvest food safety chemicals in food animals. Objective 2: Determine the fate of endogenous animal hormones, novel pre-harvest food safety compounds, and antibiotics in animal wastes, including their transport through soil and water, and develop intervention strategies that reduce their environmental impact. Objective 3: Develop sensitive and accurate analytical tools to rapidly detect and quantify agriculturally important chemicals studied under objectives 1 and 2. |
| INNOVATIVE ANIMAL MANURE TREATMENT TECHNOLOGIES FOR ENHANCED ENVIRONMENTAL QUALITY | 0409671 | SZOGI A A | 04/03/2005 | 04/02/2010 | COMPLETE | FLORENCE | ANIMAL, WASTE, WATER, QUALITY, PHOSPHORUS, NITROGEN, TRACE, ELEMENTS, AMMONIA, NITRIFICATION, DENITRIFICATION, SOLIDS, REMOVAL, WETLANDS, REDOX, POTENTIAL, OXYGEN, BOD, WETLAND, PLANTS | Not applicable | Develop and evaluate environmentally superior technologies to prevent off-farm release of nutrients and to reduce pathogens, odors, and ammonia emissions. Develop information and technologies to enhance or retrofit existing manure treatment systems to help producers meet environmental criteria (nutrients, emissions, and pathogens). Improve and refine constructed natural treatment technologies to effectively manage nutrients including reducing emissions of ammonia and nitrous oxide. Develop and evaluate new and improved technologies that concentrate/sequester nutrients from manures or create value added products including conversion of livestock waste to energy. Evaluate swine wastewater treatment systems that can be used to reduce emissions, manage nutrients, and control pathogens on small farms. Develop cooperative activities as needed to conduct the research. |
| Microbial Processes for Bioproducts and Biofuels Production | 0231133 | Liu, Yan | 09/01/2012 | 08/31/2017 | COMPLETE | EAST LANSING | algal cultivation, biofuels, biopesticide, bioproducts, chitosan, enzyme, fungal fermentation, high value protein, lipid, mix culture, transgenic algae | Biobased fuels and chemicals can make important contributions to U.S. energy security, rural economic development, and the environment. Heterotrophic conversion of organic substances (fungi and bacteria) and autotrophic conversion of inorganic compounds (algae and cyanobacteria) are two major microbial systems to produce these biofuels and chemicals. Numerous studies have been conducted in the past several decades. However, significant challenges still exist in successful realization of these microbial processes for biofuels and chemical production The recalcitrant structure of organic substances (lignocellulosic materials), dispersed nature of energy crops and agricultural residues, and limited capacity of current available industrial strains to co-utilize C5 and C6 sugars, are main barriers for heterotrophic conversion; while, long-term system stability, water and nutrient requirements, and harvesting of biomass hurdle autotrophic conversion. Addressing these challenges should be of the highest research priority in order to develop next-generation biofuels and chemicals. In response to researching and developing new routes towards effective and sustainable biofuels/chemical production systems, my research foci are mainly on heterotrophic fungal platform and autotrophic algal platform. Studies on the fungal platform include fungal cellulosic enzyme production, fungal biojet conversion, and fungi-based pesticides production, and studies on the algal platform include mixture culture of algal assemblage for lipid accumulation and water reclamation, and transgenic algal strains for pharmaceutical/neutraceutical production. The outcomes of the proposed research will lead to novel bioprocesses for biofuel/chemical production with minimum water/nutrient/energy consumption. The implementation of these processes will create great economic value for the agricultural industry, and further stimulate job creation, farm profit, and rural development. | The long-term research goal is to develop environmentally benign bioprocesses to effectively utilize various renewable resources (crop residues, animal wastes, industrial organic wastes and carbon dioxide) for value-added energy/chemical production, with a specific aim towards making scientific and technological advances to meet demands of the emerging bioeconomy. The objective of the proposed research is to demonstrate novel fuel/chemical production systems that apply advanced fungal and algal cultivation technologies to produce enzymes, lipids, biopesticides from agricultural/industrial wastes. The objective will be achieved by pursuing following specific aims under fungal and algal platforms in five years. Specific Aims for Fungal Platform: 1. Investigating enzyme production using pelletized fungal culture; 2. Enhancing lipid accumulation in fungal biomass; 3. Enhancing biopesticide (chitosan) production from fungal cultivation. Specific Aims for Algal Platform: 1. Constructing algal/bacterial consortium to improve lipid accumulation and facilitate biomass precipitation; 2. Developing a culture strategy to enhance lipid/starch accumulation; 3. Developing transgenic algal culture for biofuels and value-added protein production. The expected outputs from the project include: 1. Peer reviewed articles and book chapters Publishing peer reviewed journal articles on those high-impact journals in the biofuels/chemical field is one of the best approaches to disseminate the research outcomes in relevant scientific communities. 2. Workshops Smaller groups of targeted parties from both academia and industries with much higher and more active engagement on specific research topics (algal or fungal related) will be invited to MSU campus. Research presentation, group discussion, system demonstration, and facility tour will be organized for the workshops to give the audience the first-hand information, and let them better understand the outcomes of the on-going biofuel/chemical research. 3. Media Potential media for biofuel/chemical research are Discovery Channel, Lansing State Journal, Biomass Products & Technology, Resource - Engineering & Technology for Sustainable World etc. 4. Industrial partners Collaborating with industrial and agricultural partnerships will enable applied and relevant research to be quickly commercialized. Considering the intellectual merits related with some of the proposed research, MSU technologies will be invited to be part of the conversation with the partners to protect potential intellectual properties. 5. Internet The research group website will be upgraded to include a dynamic web-based database. All updated research news and outcomes, educational and training materials will be updated in a timely manner. A much larger audience from different area such as agriculture, food/pharmaceutical/biofuels industries, K-16 educators, and general public will be targeted by the internet dissemination approach. |
| Accelerated Renewable Energy | 0228524 | MARKLEY, JOHN | 07/15/2012 | 07/14/2017 | COMPLETE | MADISON | Bio-diesel Bio-gas Ethanol, bio-diesel, bio-gas, cellulosic Fertilizer, custom Manure, dairy Polymer separations, economic analysis, ethanol, cellulosic, fertilizer, custom, manure, dairy, polymer separations, precision-ag | A dairy with 1,700 cows produces 15 tons of manure per day. To handle the manure, the dairy must recycle 2.5 million gallons of water per day. The conventional solutions to these problems are wash the manure into a lagoon, dredge and manure solids and haul them to fields. Manure on the fields may not provide the correct nutrients and is subject to running off and polluting rivers. Our goal is to demonstrate the economic feasibility on the scale of a large dairy farm (1,700) cows of converting the manure produced into valuable commodities including methane gas for heating purposes in the farm, fuel ethanol, and custom fertilizer. Part of the farm acreage (5%) will be devoted to oilseed production, which will be converted to biodiesel to power vehicles on the farm. Our approach utilizes biomass processing technology developed by a small Wisconsin business (Soil Net) and engineering and fabrication expertise of another small Wisconsin business (Braun Electric). We foresee a strong potential for commercialization of this technology and its widespread adoption. | We propose a public (University of Wisconsin-Madison) and private (Cottonwood Dairy; Soil Net, LLC; Braun Electric; Resource Engineering Associates, Inc.) collaboration that encompasses both R&D and prototypical farm-based demonstration of the four components of the BRDI FOA: 1. Feedstocks Development: The bioenergy generated will derive primarily from recycled cellulosic components of dairy manure, which have minimal food/fuel issues. 2. Bio-Fuels and Bio-based Products Development: The project will demonstrate/evaluate multiple sub-processes and associated "value added" bio-based co-products -- vegetable oil/meal; oil/biodiesel; cellulosic ethanol; bio-gas/manure digestion; recycled rinse water; low and high P (phosphorus) crop nutrients; and multiple cellulosic manure fiber "fractions" (for mulches, bedding, etc.). 3. Bio-Fuels and Bio-based Products Development Analysis: The project will evaluate (calibrate, implement, validate) economic, environmental, lifecycle, process efficiency, and mass balance analysis and incorporate these into a business decision/management framework. In particular, an analysis of the economics of scale of the various system components will form a major part of the research effort. 4. Use of Oil/Biodiesel for the Production of Grain or Cellulosic Ethanol: The proposed system will be capable of producing oil/biodiesel from vegetable oil seed produced on the farm. Our research will determine the economic benefits of biodiesel vs. purified vegetable oil for direct use in operating farm vehicles and machinery. The expected outcome is the demonstration of cost effective livestock manure separation and processing to produce bio-energy, bio-feedstocks, and value added co-products (mulch/fertilizers) for on-farm and off-farm ("export") markets that can be carried out at a variety of large/medium/small scales. This technology will provide opportunities to exploit readily available, relatively low value potential cellulosic bio-feedstocks-ones that largely avoid food/fuel concerns-to improve economic sustainability: on-farm substitution for purchased energy and feed/fertilizer nutrients or as potential farm revenue diversification; improve environmental sustainability. The approach will reduce GHG/carbon footprint, soil/nutrient losses, and potential manure borne pathogens; and, improve regional economic development. We have shown that a demand exists for many of the manure fiber (mulch/fertilizer) co-products. The flexibility to adopt one (or several) of process/flow components, sequentially, based on the specifics of extant farm infrastructure (manure type/volumes, manure handling/processing, etc.) increases the proposed project's commercialization potential. The extensive process/flow measurement and analysis R&D, at both lab/bench and commercial scale, will provide the analytic/measurement tools to evaluate the economic, environmental, food safety, and regional economic development impacts of this potential commercialization at a variety of resolutions (farm, county, region). |
| A Biogas Heat Engine for Small to Mid-Sized Farms | 0226184 | Tesar, Joseph | 09/01/2011 | 02/28/2015 | COMPLETE | Ann Arbor | anaerobic digester, pathogen-free effluent, renewable energy,, biogas,, co-feeds., energy scavenging,, heat pump,, solar thermal,, tes,, thermal storage, | Non-technical Summary: The profitability of small and mid-sized dairy farms is strongly affected by increases in feed costs and energy costs. Unfortunately, farm operators have limited control of these factors, especially in the long term. New technology is needed to allow farmers to manage energy costs on their farms. One excellent solution is to use existing organic farm waste material to create energy via anaerobic digestion. Dairy operations (as well as other feeding sites) create copious amount of manure each day. By collecting this waste into an anaerobic digester, valuable biogas can be created and used by the farmer for pasteurization or hot water generation. Other organic materials can be used as feedstocks to enhance biogas production. The Biogas Heat Engine from Quantalux is an energy solution that generates valuable biogas from agricultural waste. Biogas has a large fraction of methane, and with suitable cleaning of the gas, can be used as a drop-in replacement for fossil fuels such as propane and natural gas. Our system includes a novel method for stabilizing biogas production using a thermal energy storage (TES). Renewable thermal sources are coupled to the Heat Engine via thermal storage cache, allowing the system to produce biogas more consistently. In order to show the viability of this technology for smaller farming operations, Quantalux will prototype and demonstrate that a simplified, thermally stable anaerobic digester system, We will show that the smaller farmer can self-generate biogas for use on his/her farm (decreasing energy costs), and that same farmer can also earn additional revenue (from selling enhanced digestate.) We also will show enhanced biogas production via the use of thermally stabilized digestion vessels, and by the addition of different farm-based feedstocks to the base manure feedstock. The Biogas Heat Engine will be marketed directly to small to mid-sized dairy farmers who seek decreased costs and a diversified revenue source. Revenues come from avoided cost of energy, the sale of compost, or from tipping fees from co-digestion materials. The Biogas Heat Engine is a way for the small farmer their energy costs while improving the health management of their farm and of the surrounding ecosystem. | Goals: During the USDA Phase II effort, Quantalux will develop an optimized anaerobic digester solution targeted to the needs of small to mid-sized farms. This solution is called the Biogas Heat Engine, and will generate valuable biogas from existing on-farm organic matter (primarily manure). Anaerobic digester performance will be enhanced by the addition of thermal energy storage (TES) technology (researched in Phase I.) TES allows the digester to be operated cost-effectively at higher temperatures, leading to more rapid and stable biogas production. Additional heat also reduces the number of pathogens in the digested material substantially. In addition to TES, Quantalux will also design and prototype remote process monitoring and will test and evaluate available organic material co-feeds can be added to the system to further enhance the quantity of biogas. A system for cleaning and storing biogas will also be developed. Objectives: This project will take a step-wise approach to developing a Phase II prototype. In the first step, initial Phase I computer models for anaerobic digestion and renewable energy sources (solar thermal and energy scavenging) will be refined. Key process monitors of the digestion process will be evaluated and a method for remote data exchange will be developed. Safe and cost-effective methods for storing and using biogas for farm processes (such as heating and cooling) will be developed. In the next step, a detailed engineering design will be developed for the key modules, including the renewable energy sources and TES module, the remote monitoring module and the biogas cleaning/storing module. In the final step, all system elements will be integrated into a scaled version of the Biogas Heat Engine and performance will be validated. The core anaerobic digester will be augmented with TES technology will assure thermal stability during the biogas generation process. Biogas production will be assessed based on a variety of feedstocks (both single feedstocks and co-feeds). The degree of pathogen reduction will be measured and evaluated for a range of pathogens. An overall objective is to maximize the potential revenue to the farmer (via biogas and pathogen-free byproducts) by implementing a comprehensive management strategy. Expected Outputs: The demonstration of the Biogas Heat Engine will show that a biogas-only, thermally stable anaerobic digester system can be viable on small to mid-sized farms. Several key ancillary technologies will be part of the demonstration, including: a remote monitoring system that simplifies and automates process control in the digester, a simplified scrubbing and gas storage system, and a thermal management system that allows for higher performance at lower cost. We will also show that co-feeding the digester using different organic feedstocks will result in higher biogas production. |
| Integration of bioproducts and bioenergy production with waste treatment | 0223293 | Hu, B | 10/01/2010 | 10/01/2013 | COMPLETE | MINNEAPOLIS | anaerobic digestion, microbial oil accumulation, nitrogen removal,, carbon dioxide mitigation,, fugal pelletization, microalgae cultivation,, phosphorus removal, | Microalgae oil has been proposed as the second generation source to produce biofuel. Its use is highly recommended in order to integrate microalgae cultivation with wastewater treatment so that nutrients in the waste streams can be the raw material for microalgae growth. Some experts even argue that this might be the only option economically feasible, compared to other methods such as open ponds or photobioreactor systems. Anaerobic digestion (AD) has been widely commercialized to treat agricultural residues for nutrient release as well as for harvesting biogas as an energy source. AD converts organic N and P to ammonia and phosphate while total N and P remain constant. Microalgae cultured on the AD effluent usually provide an ideal combination with the AD to utilize the remaining N and P while biomass/oil can be accumulated via microalgae cultivation. However, this process faces several challenges: 1) it is of extremely low efficiency due to the slow growth of methanogens and autotrophic microalgae. The vulnerable nature of the methanogens makes the AD process constantly unstable, while the rich organic nutrients and high turbidity in the AD effluent actually inhibit microalgae to grow on sun light and CO2. 2) The biogas produced from the system consists over 50% impurities such as CO2 etc, which dramatically increase its application on high valued market. 3) The harvest of microalgae is energy-intensive, which is one of the major factors inhibiting the commercialization of the process. To solve the above mentioned issues, firstly, an integrated Anaerobic Digestion and Oil/Biomass Accumulation (ADOBA) process is proposed to combine the acitogenesis/fermentation stage of the anaerobic digestion (AD) process directly with the oil accumulation via mixotrophic microalgae or fungal cultivation. It is a simplified process, derived from common waste-water treatment processes such as AD or AD followed by microalgae cultivation in stabilization ponds (Fig. 1). Compared to these environmental processes, ADOBA will be more suitable for bio-energy production for the following reasons: 1) ADOBA has the same first acitogenesis step as AD, so ADOBA will provide the same benefits as AD in many aspects, including production of renewable energy, reduction of greenhouse gas (GHG) emissions, and potential pathogen reduction. 2). ADOBA will degrade organics much faster than the AD followed by microalgae culture, because without the rate-limiting methanogenesis step, the acitogenesis step of the AD will only serve as the pre-treatment of waste materials and the organic nutrients such as VFA will stimulate the fast growth rate of heterotrophic microalgae cultivation. Secondly, ADOBA-microalage process is proposed to utilize microalgae for the carbon dioxide capture. With the integration of microalgae cultivation with AD, the biogas can be relatively purified via CO2 assimilation with microalgae. Finally, taking advantage of fungal pelletization and its merit on liquid/solid separation, ADOBA-fungi process is proposed to accumulate oil via pelletized cell culture, so that fat cells can be easily harvested. | Research goals An innovative two step Anaerobic Digestion and Oil/Biomass Accumulation (ADOBA) process (Fig. 1) is proposed to integrate fermentative hydrogen production directly with either mixotrophic/autotrophic microalgae cultivation for oil accumulation (ADOBA-microalgae) or with fungal cultivation (ADOBA-fungi). The first step is the acitogenesis/hydrogen fermentation, where organic materials are degraded to produce H2/biogas and volatile fatty acid (VFA); and then in the second step, the effluent from the fermentation will be processed to culture microalgae or fungi for the oil synthesis, where the nutrients such as VFA, N and P will be utilized. Impurities of the biogas from the first stage, such as CO2, NH3 and H2S, will be able to be assimilated and cleaned via microalgae growth. The proposed ADOBA process will provide a new application of the VFA, N and P from water, an innovative method to remove impurities from biogas and a unique way to separate the cell biomass, all of which will increase the economic feasibility of the biological hydrogen production process. Objectives and expected outputs The project will be focusing on the feasibility study of the proposed process. For the ADOBA-microalage process, our primary focus is to study hydrogen gas purification via microalgae cultivation. Our hypothesis of this research is that carbon dioxide will be totally removed from the biogas without oxygen production, therefore, the biogas can be purified. The whole process will be integrated and optimized for their culture conditions. Our goal of the process is to produce around 2 mole H2 per mole glucose, completely assimilate VFA, N and P by microalgae cultivation, dramatically decrease the microalgae cultivation time, increase the oil content to 40-50%, and purify H2 produced from the system to reach 90%. In addition, for the ADOBA-fungi process, a new concept of pelletized/granulated cell cultivation will be adventured for valuable bioproducts and bioenergy production due to above merits. Application of cell aggregates to oil production depends upon obtaining uniform pellets of a desired size. This is not easily accomplished, since many factors influence pellet formation. Filamentous oleaginous fungi Aspergillus oryzae or Mortierella isabellina will be chosen as a model to test our research hypothesis: the pellet will be formed on these fungal fermentation and the pelletized culture will significantly facilitate the harvest of the cell biomass, and decrease the overall cost of the microbial oil accumulation process. |
| Animal Manure and Waste Utilization, Treatment and Nuisance Avoidance for a Sustainable Agriculture | 0191080 | Theegala, C. | 10/01/2001 | 09/30/2007 | COMPLETE | SHREVEPORT | livestock, pollution control, animal waste, water quality, environmental impact, manures, waste treatment, sustainable agriculture, agricultural engineering, waste management, land application, optimization, waste utilization, process development, dairy cattle, pastures, fecal coliforms, runoff, rain simulation, measurement, monitoring, nitrogen, phosphorus, nutrient balance, crop residues, aquaculture, composting, automation, control systems | Grazing of cattle and land application of animal manure are common agricultural practices. Assessment of pollutant transport from grazed pastures and development of treatment options for animal manures are needed in order to protect Louisiana's water resources. The water quality impacts of grazing dairy cattle on pasture will be studied in regard to fecal coliform content in runoff water. As part of an ongoing study, pasture plots will be artificially dosed with dairy cow manure in a manner similar to natural deposition during grazing. | 1. Develop management tools, strategies, and systems for land application of animal manures and effluents that optimize efficient, environmentally friendly utilization of nutrients and are compatible with sustained land and water quality. 2. Develop, evaluate, and refine physical, chemical and biological treatment processes in engineered and natural systems for management of manures and other wastes. |
| Post Harvest Food Safety | 0184379 | Aramouni, F. | 11/01/1999 | 12/31/2005 | COMPLETE | MANHATTAN | meat, food microbiology, food safety, post harvest, food contamination, pesticide residues, food processing, meat processing, technology transfer, intervention, food quality, knowledge, information dissemination, best management practices, pathogen characterization, sanitation, training, irradiation, heat treatment, fusarium, mycotoxins | Promote a Safe Food Suppy from Production to Consumption. | 1. The Kansas food and meat processing industry will adopt technologies and intervention strategies that will result in a safer, more wholesome food supply. 2. Industry/commodity groups, meat and food processors, regulatory agencies (FDA, FSIS), and consumer groups will increase knowledge and understanding of food safety principles and practices to support enhancement of their respective roles in assuring a safe, wholesome food supply. 3. Food and meat processing operations will improve microbiological counts as a result of sanitation and HACCP training and implementation. |